ID IF4A_MAIZE Reviewed; 410 AA.
AC Q41741;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 27-MAR-2024, entry version 129.
DE RecName: Full=Eukaryotic initiation factor 4A;
DE Short=eIF-4A;
DE EC=3.6.4.13;
DE AltName: Full=ATP-dependent RNA helicase eIF4A;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Ear of corn;
RX PubMed=7630958; DOI=10.1104/pp.108.3.1317;
RA Jayachandran S., Bailey-Serres J.;
RT "Nucleotide sequence of a cDNA for the maize protein synthesis initiation
RT factor 4A.";
RL Plant Physiol. 108:1317-1318(1995).
CC -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the eIF4F
CC complex involved in cap recognition and is required for mRNA binding to
CC ribosome. In the current model of translation initiation, eIF4A unwinds
CC RNA secondary structures in the 5'-UTR of mRNAs which is necessary to
CC allow efficient binding of the small ribosomal subunit, and subsequent
CC scanning for the initiator codon (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of which
CC varies with external and internal environmental conditions. It is
CC composed of at least EIF4A, EIF4E and EIF4G (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A subfamily.
CC {ECO:0000305}.
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DR EMBL; U17979; AAA82736.1; -; mRNA.
DR AlphaFoldDB; Q41741; -.
DR SMR; Q41741; -.
DR STRING; 4577.Q41741; -.
DR PaxDb; 4577-GRMZM2G027995_P02; -.
DR MaizeGDB; 112980; -.
DR eggNOG; KOG0327; Eukaryota.
DR InParanoid; Q41741; -.
DR Proteomes; UP000007305; Unplaced.
DR ExpressionAtlas; Q41741; baseline and differential.
DR GO; GO:0010494; C:cytoplasmic stress granule; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR CDD; cd17939; DEADc_EIF4A; 1.
DR CDD; cd18787; SF2_C_DEAD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR PANTHER; PTHR47958; ATP-DEPENDENT RNA HELICASE DBP3; 1.
DR PANTHER; PTHR47958:SF1; EUKARYOTIC TRANSLATION INITIATION FACTOR EIF-4A; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome; RNA-binding.
FT CHAIN 1..410
FT /note="Eukaryotic initiation factor 4A"
FT /id="PRO_0000054962"
FT DOMAIN 68..238
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 249..410
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 37..65
FT /note="Q motif"
FT MOTIF 186..189
FT /note="DEAD box"
FT BINDING 81..88
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 410 AA; 46536 MW; B9B8AB78ED3CE184 CRC64;
MAGLAPEGSQ FDDKQYDKKM QEILTEDFFT SYDDVCESFD SMGLQENLLR GIYAYGFEKP
SAIQQRGIVP FCKGLDVIQQ AQSGTGKTAT FCSGILQQLD YGLVECQALV LAPTRELAQQ
IEKVMRALGD YLGVKVHACV GGTSVREDQR ILASGVHVVV GTPGRVFDML RRQSLRPDNI
KMFVLDEADE MLSRGFKDQI YDIFQLLPSK IQVGVFSATM PPEALEITRK FMNKPVRILV
KRDELTLEGI KQFYVNIDKE DWKLDTLCDL YETLAITQSV IFVNTRRKVD WLTDKMRSRD
HTVSATHGDM DQNTRDIIMR EFRSGSSRVL ITTDLLARGI DVQQVSLVIN YDLPTQPENY
LHRIGRSGRF GRKGVAINFV TRDDERIVFD VQRFYNVTVE ELPANVADLL
//
