ID Q46707_ECOLX Unreviewed; 177 AA.
AC Q46707;
DT 01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 153.
DE RecName: Full=phospholipase D {ECO:0000256|ARBA:ARBA00012027};
DE EC=3.1.4.4 {ECO:0000256|ARBA:ARBA00012027};
GN Name=nuc {ECO:0000313|EMBL:AAA86461.1};
GN ORFNames=EIZ93_17635 {ECO:0000313|EMBL:MQK26106.1}, FTV92_25330
GN {ECO:0000313|EMBL:QED72448.1}, G8C01_24205
GN {ECO:0000313|EMBL:MBE9994392.1}, pMUR050_045
GN {ECO:0000313|EMBL:ABG33821.1}, RCS45_P0022
GN {ECO:0000313|EMBL:SPD98855.1};
OS Escherichia coli.
OG Plasmid RCS45_p {ECO:0000313|EMBL:SPD98855.1},
OG Plasmid pH1038-142 {ECO:0000313|EMBL:AIF78280.1},
OG Plasmid pHHA45 {ECO:0000313|EMBL:AFP24965.1},
OG Plasmid pKM101 {ECO:0000313|EMBL:AAA86461.1},
OG Plasmid pL2-43 {ECO:0000313|EMBL:AIF79346.1},
OG Plasmid pLEW517 {ECO:0000313|EMBL:ABG49201.1},
OG Plasmid pMUR050 {ECO:0000313|EMBL:ABG33821.1},
OG Plasmid pNCYU-25-82-1_MCR3 {ECO:0000313|EMBL:QED72448.1},
OG Plasmid pQNR2078 {ECO:0000313|EMBL:CCE73956.1}, and
OG Plasmid pncyu-25-82-1_mcr3 {ECO:0000313|Proteomes:UP000321760}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562 {ECO:0000313|EMBL:AAA86461.1};
RN [1] {ECO:0000313|EMBL:AAA86461.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pKM101 {ECO:0000313|EMBL:AAA86461.1};
RX PubMed=7891554; DOI=10.1111/j.1365-2958.1994.tb01304.x;
RA Pohlman R.F., Genetti H.D., Winans S.C.;
RT "Common ancestry between IncN conjugal transfer genes and macromolecular
RT export systems of plant and animal pathogens.";
RL Mol. Microbiol. 14:655-668(1994).
RN [2] {ECO:0000313|EMBL:AAA86461.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pKM101 {ECO:0000313|EMBL:AAA86461.1};
RX PubMed=8029323; DOI=10.1006/plas.1994.1017;
RA Pohlman R.F., Genetti H.D., Winans S.C.;
RT "Entry exclusion of the IncN plasmid pKM101 is mediated by a single
RT hydrophilic protein containing a lipid attachment motif.";
RL Plasmid 31:158-165(1994).
RN [3] {ECO:0007829|PDB:1BYR}
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 23-177.
RX PubMed=10074947; DOI=10.1038/6716;
RA Stuckey J.A., Dixon J.E.;
RT "Crystal structure of a phospholipase D family member.";
RL Nat. Struct. Biol. 6:278-284(1999).
RN [4] {ECO:0000313|EMBL:ABG33821.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pMUR050 {ECO:0000313|EMBL:ABG33821.1};
RX PubMed=15963296;
RA Gonzalez-Zorn B., Teshager T., Casas M., Porrero M.C., Moreno M.A.,
RA Courvalin P., Dominguez L.;
RT "armA and aminoglycoside resistance in Escherichia coli.";
RL Emerg. Infect. Dis. 11:954-956(2005).
RN [5] {ECO:0000313|EMBL:ABG33821.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pMUR050 {ECO:0000313|EMBL:ABG33821.1};
RX PubMed=16027145; DOI=10.1093/jac/dki246;
RA Gonzalez-Zorn B., Catalan A., Escudero J.A., Dominguez L., Teshager T.,
RA Porrero C., Moreno M.A.;
RT "Genetic basis for dissemination of armA.";
RL J. Antimicrob. Chemother. 56:583-585(2005).
RN [6] {ECO:0000313|EMBL:ABG49201.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=517-2H1 {ECO:0000313|EMBL:ABG49201.1};
RC PLASMID=pLEW517 {ECO:0000313|EMBL:ABG49201.1};
RX PubMed=16820486; DOI=10.1128/AEM.00354-06;
RA Williams L.E., Detter C., Barry K., Lapidus A., Summers A.O.;
RT "Facile recovery of individual high-molecular-weight, low-copy-number
RT natural plasmids for genomic sequencing.";
RL Appl. Environ. Microbiol. 72:4899-4906(2006).
RN [7] {ECO:0000313|EMBL:ABG33821.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pMUR050 {ECO:0000313|EMBL:ABG33821.1};
RA Gonzalez-Zorn B., Catalan A., Moreno M.A., Dominguez L.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [8] {ECO:0000313|EMBL:CCE73956.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=2078 {ECO:0000313|EMBL:CCE73956.1};
RC PLASMID=pQNR2078 {ECO:0000313|EMBL:CCE73956.1};
RA Schwarz S.P.;
RL Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases.
RN [9] {ECO:0000313|EMBL:CCE73956.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=2078 {ECO:0000313|EMBL:CCE73956.1};
RX PubMed=22334601; DOI=10.1093/jac/dks024;
RA Schink A.K., Kadlec K., Schwarz S.;
RT "Detection of qnr genes among Escherichia coli isolates of animal origin
RT and complete sequence of the conjugative qnrB19-carrying plasmid
RT pQNR2078.";
RL J. Antimicrob. Chemother. 67:1099-1102(2012).
RN [10] {ECO:0000313|EMBL:AFP24965.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=HHA45 {ECO:0000313|EMBL:AFP24965.1};
RC PLASMID=pHHA45 {ECO:0000313|EMBL:AFP24965.1};
RX PubMed=23060365; DOI=10.1093/jac/dks387;
RA Dolejska M., Villa L., Hasman H., Hansen L., Carattoli A.;
RT "Characterization of IncN plasmids carrying blaCTX-M-1 and qnr genes in
RT Escherichia coli and Salmonella from animals, the environment and humans.";
RL J. Antimicrob. Chemother. 68:333-339(2013).
RN [11] {ECO:0000313|EMBL:AIF78280.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Human-1038 {ECO:0000313|EMBL:AIF78280.1}, and Lamb-2
RC {ECO:0000313|EMBL:AIF79346.1};
RC PLASMID=pH1038-142 {ECO:0000313|EMBL:AIF78280.1}, and pL2-43
RC {ECO:0000313|EMBL:AIF79346.1};
RX PubMed=24920651; DOI=10.1093/jac/dku206;
RA Wang J., Stephan R., Power K., Yan Q., Hachler H., Fanning S.;
RT "Nucleotide sequences of 16 transmissible plasmids identified in nine
RT multidrug-resistant Escherichia coli isolates expressing an ESBL phenotype
RT isolated from food-producing animals and healthy humans.";
RL J. Antimicrob. Chemother. 69:2658-2668(2014).
RN [12] {ECO:0000313|EMBL:SPD98855.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=502 {ECO:0000313|EMBL:SPD98855.1};
RC PLASMID=RCS45_p {ECO:0000313|EMBL:SPD98855.1};
RA Cea G.-C., William W.;
RL Submitted (FEB-2018) to the EMBL/GenBank/DDBJ databases.
RN [13] {ECO:0000313|EMBL:MQK26106.1, ECO:0000313|Proteomes:UP000359125}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A016f {ECO:0000313|EMBL:MQK26106.1,
RC ECO:0000313|Proteomes:UP000359125};
RX PubMed=31642700; DOI=.1289/EHP5251;
RA Li J., Bi Z., Ma S., Chen B., Cai C., He J., Schwarz S., Sun C., Zhou Y.,
RA Yin J., Hulth A., Wang Y., Shen Z., Wang S., Wu C., Nilsson L.E.,
RA Walsh T.R., Borjesson S., Shen J., Sun Q., Wang Y.;
RT "Inter-host Transmission of Carbapenemase-Producing Escherichia coli among
RT Humans and Backyard Animals.";
RL Environ. Health Perspect. 127:107009-107009(2019).
RN [14] {ECO:0000313|EMBL:QED72448.1, ECO:0000313|Proteomes:UP000321760}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCYU-25-82 {ECO:0000313|EMBL:QED72448.1,
RC ECO:0000313|Proteomes:UP000321760};
RC PLASMID=pNCYU-25-82-1_MCR3 {ECO:0000313|EMBL:QED72448.1}, and
RC pncyu-25-82-1_mcr3 {ECO:0000313|Proteomes:UP000321760};
RA Hsu C.-Y., Huang W.-C., Lauderdale T.-L.;
RT "Plasmid- and chromosome-located mcr-3 in mcr-1-positive Escherichia coli
RT from diseased swine, Taiwan.";
RL Submitted (AUG-2019) to the EMBL/GenBank/DDBJ databases.
RN [15] {ECO:0000313|EMBL:QED72448.1, ECO:0000313|Proteomes:UP000321760}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCYU-25-82 {ECO:0000313|EMBL:QED72448.1,
RC ECO:0000313|Proteomes:UP000321760};
RC PLASMID=pncyu-25-82-1_mcr3 {ECO:0000313|Proteomes:UP000321760}, and
RC pNCYU-25-82-1_MCR3 {ECO:0000313|EMBL:QED72448.1};
RA Chen F.-J., Wu H.-C., Liao Y.-C., Kuo S.-C.;
RL Submitted (AUG-2019) to the EMBL/GenBank/DDBJ databases.
RN [16] {ECO:0000313|EMBL:MBE9994392.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=6D1-6 {ECO:0000313|EMBL:MBE9994392.1};
RA Fatoba D., Amoako D.G., Abia Akebe L.K., Essack S.;
RT "Genomic Epidemiology of Antibiotic-Resistant Bacteria from Agricultural
RT Soil Fertilized with Poultry Manure in KwaZulu Natal, South Africa.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-
CC sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:14445,
CC ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57643, ChEBI:CHEBI:58608; EC=3.1.4.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000798};
CC -!- SIMILARITY: Belongs to the phospholipase D family.
CC {ECO:0000256|ARBA:ARBA00008664}.
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DR EMBL; U09868; AAA86461.1; -; Genomic_DNA.
DR EMBL; AY522431; ABG33821.1; -; Genomic_DNA.
DR EMBL; DQ390454; ABG49201.1; -; Genomic_DNA.
DR EMBL; JX065630; AFP24965.1; -; Genomic_DNA.
DR EMBL; KJ484634; AIF78280.1; -; Genomic_DNA.
DR EMBL; KJ484641; AIF79346.1; -; Genomic_DNA.
DR EMBL; HE613857; CCE73956.1; -; Genomic_DNA.
DR EMBL; JAAMTB010000053; MBE9994392.1; -; Genomic_DNA.
DR EMBL; RYCF01000069; MQK26106.1; -; Genomic_DNA.
DR EMBL; CP042628; QED72448.1; -; Genomic_DNA.
DR EMBL; LT985251; SPD98855.1; -; Genomic_DNA.
DR PIR; I79276; I79276.
DR RefSeq; WP_000731968.1; NZ_WSWX01000056.1.
DR RefSeq; YP_001096362.1; NC_009132.1.
DR RefSeq; YP_006903017.1; NC_019033.1.
DR RefSeq; YP_006954498.1; NC_019098.1.
DR RefSeq; YP_009061039.1; NC_024974.1.
DR RefSeq; YP_009068534.1; NC_025141.1.
DR RefSeq; YP_724502.1; NC_007682.3.
DR PDB; 1BYR; X-ray; 2.00 A; A=23-177.
DR PDBsum; 1BYR; -.
DR GeneID; 76525224; -.
DR PATRIC; fig|562.9077.peg.5045; -.
DR Proteomes; UP000321760; Plasmid pncyu-25-82-1_mcr3.
DR Proteomes; UP000359125; Unassembled WGS sequence.
DR Proteomes; UP000701764; Unassembled WGS sequence.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006793; P:phosphorus metabolic process; IEA:UniProt.
DR CDD; cd09170; PLDc_Nuc; 1.
DR Gene3D; 3.30.870.10; Endonuclease Chain A; 1.
DR InterPro; IPR025202; PLD-like_dom.
DR InterPro; IPR001736; PLipase_D/transphosphatidylase.
DR PANTHER; PTHR43856; CARDIOLIPIN HYDROLASE; 1.
DR PANTHER; PTHR43856:SF1; MITOCHONDRIAL CARDIOLIPIN HYDROLASE; 1.
DR Pfam; PF13091; PLDc_2; 1.
DR SMART; SM00155; PLDc; 1.
DR SUPFAM; SSF56024; Phospholipase D/nuclease; 1.
DR PROSITE; PS50035; PLD; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:1BYR};
KW Endonuclease {ECO:0000313|EMBL:AAA86461.1};
KW Hydrolase {ECO:0000313|EMBL:AAA86461.1};
KW Nuclease {ECO:0000313|EMBL:AAA86461.1};
KW Plasmid {ECO:0000313|EMBL:AAA86461.1}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..177
FT /note="phospholipase D"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5015097571"
FT DOMAIN 111..138
FT /note="PLD phosphodiesterase"
FT /evidence="ECO:0000259|PROSITE:PS50035"
SQ SEQUENCE 177 AA; 19445 MW; E0EFD6133C328F3E CRC64;
MKKLATWLLA AAFTTAALPA FAVEPSVQVG YSPEGSARVL VLSAIDSAKT SIRMMAYSFT
APDIMKALVA AKKRGVDVKI VIDERGNTGR ASIAAMNYIA NSGIPLRTDS NFPIQHDKVI
IVDNVTVETG SFNFTKAAET KNSENAVVIW NMPKLAESFL EHWQDRWNQG RDYRSSY
//
