ID GPDA_CUPPJ Reviewed; 347 AA.
AC Q476J3;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 01-MAY-2013, entry version 65.
DE RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD(P)+];
DE EC=1.1.1.94;
DE AltName: Full=NAD(P)H-dependent glycerol-3-phosphate dehydrogenase;
GN Name=gpsA; OrderedLocusNames=Reut_A0308;
OS Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes
OS eutrophus) (Ralstonia eutropha).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=264198;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JMP134 / LMG 1197;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M.,
RA Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.;
RT "Complete sequence of chromosome 1 of Ralstonia eutropha JMP134.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + NAD(P)(+) =
CC glycerone phosphate + NAD(P)H.
CC -!- PATHWAY: Membrane lipid metabolism; glycerophospholipid
CC metabolism.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate
CC dehydrogenase family.
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DR EMBL; CP000090; AAZ59690.1; -; Genomic_DNA.
DR RefSeq; YP_294534.1; NC_007347.1.
DR ProteinModelPortal; Q476J3; -.
DR STRING; 264198.Reut_A0308; -.
DR EnsemblBacteria; AAZ59690; AAZ59690; Reut_A0308.
DR GeneID; 3609853; -.
DR KEGG; reu:Reut_A0308; -.
DR PATRIC; 20226057; VBIRalEut24049_0906.
DR eggNOG; COG0240; -.
DR HOGENOM; HOG000246853; -.
DR KO; K00057; -.
DR OMA; KGIEHGT; -.
DR ProtClustDB; PRK00094; -.
DR BioCyc; CPIN264198:GIW3-309-MONOMER; -.
DR UniPathway; UPA00940; -.
DR GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0047952; F:glycerol-3-phosphate dehydrogenase [NAD(P)+] activity; IEA:HAMAP.
DR GO; GO:0004367; F:glycerol-3-phosphate dehydrogenase [NAD+] activity; IEA:InterPro.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0046167; P:glycerol-3-phosphate biosynthetic process; IEA:HAMAP.
DR GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
DR GO; GO:0006650; P:glycerophospholipid metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0008654; P:phospholipid biosynthetic process; IEA:HAMAP.
DR Gene3D; 1.10.1040.10; -; 1.
DR Gene3D; 3.40.50.720; -; 1.
DR HAMAP; MF_00394; NAD_Glyc3P_dehydrog; 1; -.
DR InterPro; IPR008927; 6-PGluconate_DH_C-like.
DR InterPro; IPR013328; DH_multihelical.
DR InterPro; IPR006168; G3P_DH_NAD-dep.
DR InterPro; IPR006109; G3P_DH_NAD-dep_C.
DR InterPro; IPR011128; G3P_DH_NAD-dep_N.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR PANTHER; PTHR11728; PTHR11728; 1.
DR Pfam; PF07479; NAD_Gly3P_dh_C; 1.
DR Pfam; PF01210; NAD_Gly3P_dh_N; 1.
DR PIRSF; PIRSF000114; Glycerol-3-P_dh; 1.
DR PRINTS; PR00077; GPDHDRGNASE.
DR SUPFAM; SSF48179; 6DGDH_C_like; 1.
DR PROSITE; PS00957; NAD_G3PDH; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Lipid biosynthesis; Lipid metabolism;
KW NAD; Oxidoreductase; Phospholipid biosynthesis;
KW Phospholipid metabolism.
FT CHAIN 1 347 Glycerol-3-phosphate dehydrogenase
FT [NAD(P)+].
FT /FTId=PRO_0000255350.
FT NP_BIND 16 21 NAD (By similarity).
FT REGION 271 272 Substrate binding (By similarity).
FT ACT_SITE 207 207 Proton acceptor (By similarity).
FT BINDING 118 118 NAD; via amide nitrogen (By similarity).
FT BINDING 118 118 Substrate (By similarity).
FT BINDING 156 156 NAD; via amide nitrogen (By similarity).
FT BINDING 271 271 NAD (By similarity).
FT BINDING 297 297 NAD (By similarity).
SQ SEQUENCE 347 AA; 35750 MW; 6CAF630AF8D85F01 CRC64;
MRRSTVRAAM KLTFLGAGAW GTALASHAAA NHDVVLWGRD PAQLAAIAAT GSNEAYLPGV
PLSPRLRVEA DFEQAVGHAA DDPDGLVVVA TPVSGLREMT RRLAGRGNGH VRMLWLCKGF
EAGTHALPHQ MVREELDAAG RTSGFDYGVL TGPSFAREVA LGLPCALTVA GSVPTLADCA
QAAFHHHAMR IYGSDDLTGV EVGGAVKNVL AIATGASDGL GLGLNARAAL VTRGLAEMTR
LGLALGGRAE TFMGLAGVGD LILTATGDLS RNRKVGQQLA AGQSLDQILA SLGHVAEGVR
CAQAVAALAE ACKIEMPITR AVCAVLFEGL SAADAVAQLL QRDARDE
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