ID Q47923_FRASE Unreviewed; 486 AA.
AC Q47923;
DT 01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=Nitrogenase molybdenum-iron protein alpha chain {ECO:0000256|ARBA:ARBA00017663};
DE EC=1.18.6.1 {ECO:0000256|ARBA:ARBA00012773};
DE AltName: Full=Dinitrogenase {ECO:0000256|ARBA:ARBA00031726};
DE AltName: Full=Nitrogenase component I {ECO:0000256|ARBA:ARBA00030899};
GN Name=nifD {ECO:0000313|EMBL:AAC18641.1};
OS Frankia sp. (strain EuIK1).
OC Bacteria; Actinomycetota; Actinomycetes; Frankiales; Frankiaceae; Frankia.
OX NCBI_TaxID=47227 {ECO:0000313|EMBL:AAC18641.1};
RN [1] {ECO:0000313|EMBL:AAC18641.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=EuIK1 {ECO:0000313|EMBL:AAC18641.1};
RA Kim H.B., An C.S.;
RT "Nucleotide sequence and expression of nifHD from Frankia strain EuIK1, a
RT symbiont of Elaeagnus umbellata.";
RL Physiol. Plantarum 99:690-695(1997).
CC -!- FUNCTION: This molybdenum-iron protein is part of the nitrogenase
CC complex that catalyzes the key enzymatic reactions in nitrogen
CC fixation. {ECO:0000256|ARBA:ARBA00002621}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=1.18.6.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000805};
CC -!- COFACTOR:
CC Name=[7Fe-Mo-9S-C-homocitryl] cluster; Xref=ChEBI:CHEBI:30409;
CC Evidence={ECO:0000256|ARBA:ARBA00001969};
CC -!- COFACTOR:
CC Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143;
CC Evidence={ECO:0000256|ARBA:ARBA00001919};
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. Forms complex with
CC the iron protein (nitrogenase component 2).
CC {ECO:0000256|ARBA:ARBA00011462}.
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family.
CC {ECO:0000256|RuleBase:RU004021}.
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DR EMBL; U53362; AAC18641.1; -; Genomic_DNA.
DR AlphaFoldDB; Q47923; -.
DR GO; GO:0016612; C:molybdenum-iron nitrogenase complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd01976; Nitrogenase_MoFe_alpha; 1.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 3.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR010143; Nase_comp1_asu.
DR InterPro; IPR000318; Nase_comp1_CS.
DR InterPro; IPR005972; Nase_Mo-Fe_asu.
DR NCBIfam; TIGR01862; N2-ase-Ialpha; 1.
DR NCBIfam; TIGR01282; nifD; 1.
DR PANTHER; PTHR43457; NITROGENASE MOLYBDENUM-IRON PROTEIN ALPHA CHAIN; 1.
DR PANTHER; PTHR43457:SF1; NITROGENASE MOLYBDENUM-IRON PROTEIN ALPHA CHAIN; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
DR PROSITE; PS00699; NITROGENASE_1_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|RuleBase:RU004021};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 64..467
FT /note="Nitrogenase/oxidoreductase component 1"
FT /evidence="ECO:0000259|Pfam:PF00148"
SQ SEQUENCE 486 AA; 54225 MW; 96BB50D9071EA8A1 CRC64;
MTTTPAPERA ETEAMIAEVL EQYPQKAAKF RAKHLKANHP EGSKECEVKS NIKSRPGVMT
IRGCRYAGSK GVVWGPVKDL VTISHGPVGC GQYSWATRRN YARGPWGVTN FTAMQITTDF
QEKDIVFGGD PNLEQVCDEI NELFPLAKGI SVQSECPIGL IGDDIEPVSA TASKKLAKPV
IPVRCEAFRG VSQSLGHHIA NEPVRDHVLG TGGESFQETP YDVALIGDYN IGGDAWASRR
ILEDMGLRVI AQWSGDGTLN EMASTHLSKL NLIHCYRSMN YICTTMEERY GTPWIEFNFF
GPTKIVNSMR AIAAKFDETI QAKTEAAIGR YQKRFDEITA AFKPRLDGKR VMLAVGGLRP
RHTIGAYEDL GMEVVGTGYE FAHKDDYTRT YSMLKEGIVL YDDPTAFELE EFAKRLKRDL
MGAGVKEKYV FHKMGIPFRQ MHSWDYSGPY HGVDGFAVFA RDMDIAINSP TWDLMETPWS
KAGEVF
//
