ID Q48TB8_STRPM Unreviewed; 436 AA.
AC Q48TB8;
DT 13-SEP-2005, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2005, sequence version 1.
DT 27-MAR-2024, entry version 93.
DE SubName: Full=16S rRNA m(5)C 967 methyltransferase {ECO:0000313|EMBL:AAX72042.1};
DE EC=2.1.1.- {ECO:0000313|EMBL:AAX72042.1};
GN OrderedLocusNames=M28_Spy0929 {ECO:0000313|EMBL:AAX72042.1};
OS Streptococcus pyogenes serotype M28 (strain MGAS6180).
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=319701 {ECO:0000313|EMBL:AAX72042.1, ECO:0000313|Proteomes:UP000009292};
RN [1] {ECO:0000313|EMBL:AAX72042.1, ECO:0000313|Proteomes:UP000009292}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGAS6180 {ECO:0000313|EMBL:AAX72042.1,
RC ECO:0000313|Proteomes:UP000009292};
RX PubMed=16088825; DOI=10.1086/430618;
RA Green N.M., Zhang S., Porcella S.F., Nagiec M.J., Barbian K.D., Beres S.B.,
RA LeFebvre R.B., Musser J.M.;
RT "Genome sequence of a serotype M28 strain of group A Streptococcus:
RT potential new insights into puerperal sepsis and bacterial disease
RT specificity.";
RL J. Infect. Dis. 192:760-770(2005).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000056; AAX72042.1; -; Genomic_DNA.
DR RefSeq; WP_011284823.1; NC_007296.2.
DR AlphaFoldDB; Q48TB8; -.
DR KEGG; spb:M28_Spy0929; -.
DR HOGENOM; CLU_005316_6_1_9; -.
DR Proteomes; UP000009292; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd21147; RsmF_methylt_CTD1; 1.
DR Gene3D; 2.30.130.60; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR031341; Methyltr_RsmF_N.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR027391; Nol1_Nop2_Fmu_2.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR031340; RsmF_methylt_CI.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22807:SF30; 28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF17125; Methyltr_RsmF_N; 1.
DR Pfam; PF13636; Methyltranf_PUA; 1.
DR Pfam; PF17126; RsmF_methylt_CI; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 1..284
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT ACT_SITE 222
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 100..106
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 124
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 169
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 436 AA; 48915 MW; 4D904741978B11CC CRC64;
MMSLPKEFIN TYQAILGKEL EDFLASFNQE PVNAFRINPL KNQLKTFEHP IPNTLWGYYG
KLSGKSPEHV SGLVYSQEPA AQMVAQVAAP QKGSRVLDLA AAPGGKSTHL LAYLDNTGLL
VSNEISKKRS KVLVENIERF GARNVVVTNE SADRLAKVFS HYFDTIVFDG PCSGEGMFRK
DPDAIQYWHH GYPAECAKLQ KSILEDALTM LKPGGELIYS TCTWAPEENE DVVQWLLETY
TFLELVDVPK LNGMVSGIGL PETARMYPHR YQGEGQFVAK LKDKRQEGQS TKLKAPKSNL
TKDQLRLWKM FEKDHLNLTL SGTLQTFGDY LYLLPDGLPA LDGLKIARNG LELGVFKKKR
FEPSYALGLA LKPDEVRSSI ELREEDFRPY VSGNVITLPQ SYPTGWYQLL INGNGLGFAK
VVDKTIKNNF PKGLRF
//
