ID Q4CPV7_TRYCC Unreviewed; 477 AA.
AC Q4CPV7;
DT 13-SEP-2005, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2005, sequence version 1.
DT 24-JAN-2024, entry version 100.
DE RecName: Full=protein-synthesizing GTPase {ECO:0000256|ARBA:ARBA00011986};
DE EC=3.6.5.3 {ECO:0000256|ARBA:ARBA00011986};
GN ORFNames=Tc00.1047053503819.30 {ECO:0000313|EMBL:EAN82311.1},
GN Tc00.1047053510359.310 {ECO:0000313|EMBL:EAN99902.1};
OS Trypanosoma cruzi (strain CL Brener).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma; Schizotrypanum.
OX NCBI_TaxID=353153 {ECO:0000313|Proteomes:UP000002296};
RN [1] {ECO:0000313|EMBL:EAN82311.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CL Brener {ECO:0000313|EMBL:EAN82311.1};
RX PubMed=16020724; DOI=10.1126/science.1112181;
RA El-Sayed N.M., Myler P.J., Blandin G., Berriman M., Crabtree J.,
RA Aggarwal G., Caler E., Renauld H., Worthey E.A., Hertz-Fowler C.,
RA Ghedin E., Peacock C., Bartholomeu D.C., Haas B.J., Tran A.N.,
RA Wortman J.R., Alsmark U.C., Angiuoli S., Anupama A., Badger J.,
RA Bringaud F., Cadag E., Carlton J.M., Cerqueira G.C., Creasy T.,
RA Delcher A.L., Djikeng A., Embley T.M., Hauser C., Ivens A.C.,
RA Kummerfeld S.K., Pereira-Leal J.B., Nilsson D., Peterson J., Salzberg S.L.,
RA Shallom J., Silva J.C., Sundaram J., Westenberger S., White O.,
RA Melville S.E., Donelson J.E., Andersson B., Stuart K.D., Hall N.;
RT "Comparative genomics of trypanosomatid parasitic protozoa.";
RL Science 309:404-409(2005).
RN [2] {ECO:0000313|EMBL:EAN82311.1, ECO:0000313|Proteomes:UP000002296}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CL Brener {ECO:0000313|EMBL:EAN82311.1,
RC ECO:0000313|Proteomes:UP000002296};
RX PubMed=16020725; DOI=10.1126/science.1112631;
RA El-Sayed N.M., Myler P.J., Bartholomeu D.C., Nilsson D., Aggarwal G.,
RA Tran A.N., Ghedin E., Worthey E.A., Delcher A.L., Blandin G.,
RA Westenberger S.J., Caler E., Cerqueira G.C., Branche C., Haas B.,
RA Anupama A., Arner E., Aslund L., Attipoe P., Bontempi E., Bringaud F.,
RA Burton P., Cadag E., Campbell D.A., Carrington M., Crabtree J., Darban H.,
RA da Silveira J.F., de Jong P., Edwards K., Englund P.T., Fazelina G.,
RA Feldblyum T., Ferella M., Frasch A.C., Gull K., Horn D., Hou L., Huang Y.,
RA Kindlund E., Klingbeil M., Kluge S., Koo H., Lacerda D., Levin M.J.,
RA Lorenzi H., Louie T., Machado C.R., McCulloch R., McKenna A., Mizuno Y.,
RA Mottram J.C., Nelson S., Ochaya S., Osoegawa K., Pai G., Parsons M.,
RA Pentony M., Pettersson U., Pop M., Ramirez J.L., Rinta J., Robertson L.,
RA Salzberg S.L., Sanchez D.O., Seyler A., Sharma R., Shetty J., Simpson A.J.,
RA Sisk E., Tammi M.T., Tarleton R., Teixeira S., Van Aken S., Vogt C.,
RA Ward P.N., Wickstead B., Wortman J., White O., Fraser C.M., Stuart K.D.,
RA Andersson B.;
RT "The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas
RT disease.";
RL Science 309:409-415(2005).
RN [3] {ECO:0000313|EMBL:EAN82311.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CL Brener {ECO:0000313|EMBL:EAN82311.1};
RA El-Sayed N., Bartholomeu D., Haas B.;
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0007829|PDB:7ASE}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.33 ANGSTROMS).
RX PubMed=33357443; DOI=10.1016/j.celrep.2020.108534;
RA Bochler A., Querido J.B., Prilepskaja T., Soufari H., Simonetti A.,
RA Del Cistia M.L., Kuhn L., Ribeiro A.R., Valasek L.S., Hashem Y.;
RT "Structural Differences in Translation Initiation between Pathogenic
RT Trypanosomatids and Their Mammalian Hosts.";
RL Cell Rep. 33:108534-108534(2020).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.3;
CC Evidence={ECO:0000256|ARBA:ARBA00001874};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EAN82311.1}.
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DR EMBL; AAHK01002572; EAN82311.1; -; Genomic_DNA.
DR EMBL; AAHK01000007; EAN99902.1; -; Genomic_DNA.
DR RefSeq; XP_804162.1; XM_799069.1.
DR RefSeq; XP_821753.1; XM_816660.1.
DR PDB; 7ASE; EM; 3.33 A; 5=1-477.
DR AlphaFoldDB; Q4CPV7; -.
DR EMDB; EMD-11893; -.
DR SMR; Q4CPV7; -.
DR STRING; 353153.Q4CPV7; -.
DR PaxDb; 353153-Q4CPV7; -.
DR EnsemblProtists; EAN82311; EAN82311; Tc00.1047053503819.30.
DR EnsemblProtists; EAN99902; EAN99902; Tc00.1047053510359.310.
DR GeneID; 3533520; -.
DR GeneID; 3554746; -.
DR KEGG; tcr:503819.30; -.
DR KEGG; tcr:510359.310; -.
DR eggNOG; KOG0466; Eukaryota.
DR InParanoid; Q4CPV7; -.
DR OrthoDB; 1512042at2759; -.
DR Proteomes; UP000002296; Unassembled WGS sequence.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR CDD; cd01888; eIF2_gamma; 1.
DR CDD; cd03688; eIF2_gamma_II; 1.
DR CDD; cd15490; eIF2_gamma_III; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 2.
DR InterPro; IPR015256; eIF2g_C.
DR InterPro; IPR044127; eIF2g_dom_2.
DR InterPro; IPR044128; eIF2g_GTP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR PANTHER; PTHR42854; EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER; 1.
DR PANTHER; PTHR42854:SF3; EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED; 1.
DR Pfam; PF09173; eIF2_C; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:7ASE};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Initiation factor {ECO:0000256|ARBA:ARBA00022540,
KW ECO:0000313|EMBL:EAN82311.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000002296}.
FT DOMAIN 50..256
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 477 AA; 52580 MW; 3F2F194899D55802 CRC64;
MDENDIADFT MDENTFVTNE QNERGLATQD INALNLDKIN AETFEVMSRQ ATVNIGTIGH
VAHGKSTVVK ALSGVKTQKY HREAVMNITI HLGYANAKVF KCDKCELPAA FHAFPSSQPD
KTDCPTCGSP LTLKRHFSFV DCPGHDVLMA TMLNGAAIMD AALLLIAANE PFPQPQTLEH
LKAVEIMRLA NLVILQNKID LVGEVHAQDQ YHKIRNYIDS TIGSNIPIIP ISAQLKRNID
YLLEYLCHIP LPTRQLNCPA HMTVVRSFDI NKPGEVDIEN LRGGVAGGTV TRGIIRVNQV
LEIRPGQVHA QTGGTFSCTP LRTRALTLKA EDNSLQYAVP GGLIAVGTTL DPTLTRQDKM
VGHMIADEGS LPEVYAEIEV QYFLFEEMVG RSKQRDRNAK RVQKLNLQET LQINVGTLTA
GATVVNITKN PDIAKLTLVT PVCCTLDEHI AISRLVEKNF RLIGWGIIRR GVPVKLN
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