ID Q4QEZ6_LEIMA Unreviewed; 763 AA.
AC Q4QEZ6;
DT 19-JUL-2005, integrated into UniProtKB/TrEMBL.
DT 19-JUL-2005, sequence version 1.
DT 27-MAR-2024, entry version 113.
DE SubName: Full=Histone acetyltransferase-like protein {ECO:0000313|EMBL:CAJ03454.1};
DE EC=2.3.1.48 {ECO:0000313|EMBL:CAJ03454.1};
GN ORFNames=LMJF_16_0240 {ECO:0000313|EMBL:CAJ03454.1};
OS Leishmania major.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leishmania.
OX NCBI_TaxID=5664 {ECO:0000313|EMBL:CAJ03454.1, ECO:0000313|Proteomes:UP000000542};
RN [1] {ECO:0000313|EMBL:CAJ03454.1, ECO:0000313|Proteomes:UP000000542}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MHOM/IL/81/Friedlin {ECO:0000313|Proteomes:UP000000542};
RX PubMed=16020728; DOI=10.1126/science.1112680;
RA Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G.,
RA Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A.,
RA Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M.,
RA Bianchettin G., Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E.,
RA Ciarloni L., Clayton C., Coulson R.M., Cronin A., Cruz A.K., Davies R.M.,
RA De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N.,
RA Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A.,
RA Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S.,
RA Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M.,
RA Masuy D., Matthews K., Michaeli S., Mottram J.C., Muller-Auer S.,
RA Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M.,
RA Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E.,
RA Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C.,
RA Rutter S., Saunders D., Schafer M., Schein J., Schwartz D.C., Seeger K.,
RA Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V.,
RA Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J.,
RA Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D.,
RA Barrell B., Myler P.J.;
RT "The genome of the kinetoplastid parasite, Leishmania major.";
RL Science 309:436-442(2005).
RN [2] {ECO:0000313|EMBL:CAJ03454.1, ECO:0000313|Proteomes:UP000000542}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MHOM/IL/81/Friedlin {ECO:0000313|Proteomes:UP000000542};
RX PubMed=22038252; DOI=10.1101/gr.122945.111;
RA Rogers M.B., Hilley J.D., Dickens N.J., Wilkes J., Bates P.A.,
RA Depledge D.P., Harris D., Her Y., Herzyk P., Imamura H., Otto T.D.,
RA Sanders M., Seeger K., Dujardin J.C., Berriman M., Smith D.F.,
RA Hertz-Fowler C., Mottram J.C.;
RT "Chromosome and gene copy number variation allow major structural change
RT between species and strains of Leishmania.";
RL Genome Res. 21:2129-2142(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in
CC tRNA = 5'-deoxyadenosine + 5-(carboxymethyl)uridine(34) in tRNA + CoA
CC + 2 H(+) + L-methionine; Xref=Rhea:RHEA:61020, Rhea:RHEA-COMP:10407,
CC Rhea:RHEA-COMP:11727, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17319, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:65315,
CC ChEBI:CHEBI:74882; Evidence={ECO:0000256|ARBA:ARBA00034985};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61021;
CC Evidence={ECO:0000256|ARBA:ARBA00034985};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: tRNA modification. {ECO:0000256|ARBA:ARBA00005217}.
CC -!- SIMILARITY: Belongs to the ELP3 family.
CC {ECO:0000256|ARBA:ARBA00005494}.
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DR EMBL; FR796412; CAJ03454.1; -; Genomic_DNA.
DR RefSeq; XP_001682102.1; XM_001682050.1.
DR AlphaFoldDB; Q4QEZ6; -.
DR SMR; Q4QEZ6; -.
DR STRING; 5664.Q4QEZ6; -.
DR EnsemblProtists; CAJ03454; CAJ03454; LMJF_16_0240.
DR GeneID; 5650569; -.
DR KEGG; lma:LMJF_16_0240; -.
DR VEuPathDB; TriTrypDB:LmjF.16.0240; -.
DR VEuPathDB; TriTrypDB:LMJFC_160007800; -.
DR VEuPathDB; TriTrypDB:LMJLV39_160007600; -.
DR VEuPathDB; TriTrypDB:LMJSD75_160007600; -.
DR eggNOG; KOG2535; Eukaryota.
DR HOGENOM; CLU_025983_2_0_1; -.
DR InParanoid; Q4QEZ6; -.
DR OMA; YLQADQW; -.
DR UniPathway; UPA00988; -.
DR Proteomes; UP000000542; Chromosome 16.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0033588; C:elongator holoenzyme complex; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; ISO:GeneDB.
DR GO; GO:0005730; C:nucleolus; ISO:GeneDB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0106261; F:tRNA uridine(34) acetyltransferase activity; IEA:RHEA.
DR GO; GO:0016573; P:histone acetylation; ISO:GeneDB.
DR GO; GO:0043007; P:maintenance of rDNA; ISO:GeneDB.
DR GO; GO:0002926; P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation; IBA:GO_Central.
DR CDD; cd04301; NAT_SF; 1.
DR Gene3D; 3.40.630.30; -; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR039661; ELP3.
DR InterPro; IPR034687; ELP3-like.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR032432; Radical_SAM_C.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR NCBIfam; TIGR01211; ELP3; 1.
DR PANTHER; PTHR11135:SF2; ELONGATOR COMPLEX PROTEIN 3; 1.
DR PANTHER; PTHR11135; HISTONE ACETYLTRANSFERASE-RELATED; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF16199; Radical_SAM_C; 1.
DR SFLD; SFLDF00344; ELP3-like; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF55729; Acyl-CoA N-acyltransferases (Nat); 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000313|EMBL:CAJ03454.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000542};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:CAJ03454.1};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
FT DOMAIN 190..451
FT /note="Elp3/MiaA/NifB-like radical SAM core"
FT /evidence="ECO:0000259|SMART:SM00729"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 741..763
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 763 AA; 85586 MW; 3B706B3DD6732432 CRC64;
MSSDSDTEGE APSGTQSHAH WNRIEHKLAN LPTLERLMQE SDPNDFSSAD VEEASRFVRD
IVATAPQCST DIEKAQRQLQ RKYRRVFKKS LLLAGYKHLL RESMKAANDV NTLVQPHQES
CDSSGALTAA AAAAVLPDME DAVSPPPPSA PGTLPSPSLK TSVVCPVLER YLVFKAPRSQ
SGVLVVTVFT SAYPDGQNFS CQWNCYYCPN EPGQPRSYLL NEPGVRRANR LEFDPYRQFE
ERVRSLVAIG HPADKVELLV LGGTWESYPH PYRERFIRDL FYAANTMFDP PRAPRRPPLD
LLQEQLLNES AQCKIIGVTL ETRPDTINPE MLVELRRFGC TRVQLGVQHT DDGILTLVNR
QSTREDTANA IKLLKDSCFK VDIHLMPDLP GASPTVDKAM FDDVLDSPYL QADQWKIYPC
QTTPFSVIEQ WYKDGKYTPY GLENLIDVLL YAKARVHPWI RINRVIRDIP VDYVLAGVEV
ANLRQLLAHK LRERGERCCC IRCREIKGDS AVTEKLKSAT PQERRYEASE GTEVFLSVEM
PTDDATILGF LRLRLNIRNW ETPFAELLPC ALIRELHVYG NLLPTYVEEA GRSHTPAAQH
SGIGQRLLQR AEAIAREEGY SQIAVISGVG VRGYYKRRGY RLLAAHRGCF LVKNLDVGDG
LTGAPTTDPQ DEPLEFSLER DEALLQRVTA NRTAPASPIP GGVPPPGVVT LAAVRTQAVA
WYGQLRTSVA QWWSARKRCR GEETEWQQRS DEEGRAEAAA RTL
//
