ID Q50LB2_9MUSC Unreviewed; 421 AA.
AC Q50LB2;
DT 07-JUN-2005, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2005, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE SubName: Full=Uncharacterized protein CG5558 {ECO:0000313|EMBL:BAD98176.1};
GN ORFNames=CG5558 {ECO:0000313|FlyBase:FBgn0083592};
OS Drosophila parabipectinata.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=186283 {ECO:0000313|EMBL:BAD98176.1};
RN [1] {ECO:0000313|EMBL:BAD98176.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=16143626; DOI=10.1534/genetics.105.041699;
RA Nozawa M., Aotsuka T., Tamura K.;
RT "A novel chimeric gene, siren, with retroposed promoter sequence in the
RT Drosophila bipectinata complex.";
RL Genetics 171:1719-1727(2005).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01023}.
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DR EMBL; AB194415; BAD98176.1; -; Genomic_DNA.
DR AlphaFoldDB; Q50LB2; -.
DR FlyBase; FBgn0083592; Dpbp\CG5558.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IEA:InterPro.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR019013; Vma21.
DR PANTHER; PTHR22807:SF4; 28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF09446; VMA21; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00023329};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 38..59
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 79..97
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 128..415
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 345
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 247
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 274
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 292
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 421 AA; 47674 MW; 488D935E12464B20 CRC64;
MGKRGKKQQA KEVVAEEDAP QALPVQRSPQ EEDTSAEVFL WLLAYSVLMF TLPFLGFYGV
RSWLLESFPD LDRFTVNCWS VLTAVVIVNL VVAMYVLKAF REKVPPPLEP VEEAEDELES
TEPKKYHLSQ ALEYLASEDW RRKELPADAS YADFLAAVKA LEEDEFMTDL QVEGVLVFHH
KWSSYWSSHE FVKQKKFILQ NKGTCLAAEL LAPPSGSTVL DMCAAPGMKT LHLCNFMKNK
GRIYAVEQST ERYRVLCNIT SEAGCDIVTP ILGDALRMNA ENCPEVEYIL VDPSCSGNGM
QSRMNVCDEE KDDQRLFKLA GLQVKILSHA MTAFPNVKRI AYCTCSLYKE ENEEVVKRCL
QVNPSFKLLS CKKALRNKWH NVGDTEYAKI GKNVLYCLPD SDLTDGIFLA LFEKRRDGEA
D
//
