ID Q54417_SERMA Unreviewed; 443 AA.
AC Q54417;
DT 01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1996, sequence version 1.
DT 24-JAN-2024, entry version 81.
DE RecName: Full=Membrane fusion protein (MFP) family protein {ECO:0000256|RuleBase:RU365093};
GN Name=hasE8000 {ECO:0000313|EMBL:BAA12016.1};
OS Serratia marcescens.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Serratia.
OX NCBI_TaxID=615 {ECO:0000313|EMBL:BAA12016.1};
RN [1] {ECO:0000313|EMBL:BAA12016.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Sr41 {ECO:0000313|EMBL:BAA12016.1};
RA Omori K.;
RT "Cloning of the hasDE8000 genes from Serratia marcescens Sr41: secretion
RT mechanism for the lipase and metalloprotease.";
RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|RuleBase:RU365093}; Single-pass membrane protein
CC {ECO:0000256|RuleBase:RU365093}.
CC -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC family. {ECO:0000256|ARBA:ARBA00009477, ECO:0000256|RuleBase:RU365093}.
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DR EMBL; D83582; BAA12016.1; -; Genomic_DNA.
DR AlphaFoldDB; Q54417; -.
DR SMR; Q54417; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR Gene3D; 2.40.30.170; -; 1.
DR Gene3D; 2.40.50.100; -; 1.
DR InterPro; IPR043602; CusB_dom_1.
DR InterPro; IPR006144; Secretion_HlyD_CS.
DR InterPro; IPR010129; T1SS_HlyD.
DR NCBIfam; TIGR01843; type_I_hlyD; 1.
DR PANTHER; PTHR30386:SF17; ALKALINE PROTEASE SECRETION PROTEIN APRE; 1.
DR PANTHER; PTHR30386; MEMBRANE FUSION SUBUNIT OF EMRAB-TOLC MULTIDRUG EFFLUX PUMP; 1.
DR Pfam; PF00529; CusB_dom_1; 1.
DR PRINTS; PR01490; RTXTOXIND.
DR PROSITE; PS00543; HLYD_FAMILY; 1.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|RuleBase:RU365093};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475,
KW ECO:0000256|RuleBase:RU365093}; Coiled coil {ECO:0000256|SAM:Coils};
KW Hydrolase {ECO:0000313|EMBL:BAA12016.1};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU365093};
KW Metalloprotease {ECO:0000313|EMBL:BAA12016.1};
KW Protease {ECO:0000313|EMBL:BAA12016.1};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU365093};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU365093};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU365093}.
FT TRANSMEM 26..44
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365093"
FT DOMAIN 35..400
FT /note="Cation efflux system protein CusB"
FT /evidence="ECO:0000259|Pfam:PF00529"
FT COILED 262..296
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 443 AA; 48409 MW; 762FD7F822BB0C44 CRC64;
MSNQSVIPGD IDALSRQFDE GRHLRLGGWL VLLGFGGFLL WGLLAPLDKG VPVSGSVVVA
GNRKAVQHPS GGVVSQIQVH EGDRVRAGQV LLLMDTVDSR TQRDALRSQQ LSNAAQQARL
QAERDGLHAI VFPALLQARR EEPEVMSLML LQQQLFTSRR AALQSELAAI AESIAGSQAM
LEGIRQSYAS KQRQKAMLQE QLGGMRKLAA QGYVARNRLL DLEGQYAQID GQASEDTGNI
GRLGRQILEL KLRAIQRREE YQKEVSSQLA EVRMKLDELD NRLAKAEADL GHTQVKASVA
GTVVGLSVFT EGGVIGAGQQ LMEIVPSDRG LQVEARIPVE LIDKVQVGLP VELLFSAFNP
STTPRVEGEV TLVGADRLTD EKSGAPYYSV RAKVSEAGLQ RLNGLEIRPG MPVEGFIRTG
ERSMMNYLFK PLTDRLHLAL TEE
//
