ID DHX15_DICDI Reviewed; 727 AA.
AC Q54NJ4;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 01-MAY-2013, entry version 69.
DE RecName: Full=Putative pre-mRNA-splicing factor ATP-dependent RNA helicase dhx15;
DE EC=3.6.4.13;
DE AltName: Full=DEAH box protein 15;
GN Name=dhx15; Synonyms=prp43; ORFNames=DDB_G0285213;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Pre-mRNA processing factor involved in disassembly of
CC spliceosomes after the release of mature mRNA (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC -!- SUBCELLULAR LOCATION: Nucleus (By similarity).
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH
CC subfamily. DDX15/PRP43 sub-subfamily.
CC -!- SIMILARITY: Contains 1 helicase ATP-binding domain.
CC -!- SIMILARITY: Contains 1 helicase C-terminal domain.
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DR EMBL; AAFI02000076; EAL64818.1; -; Genomic_DNA.
DR RefSeq; XP_638323.1; XM_633231.1.
DR ProteinModelPortal; Q54NJ4; -.
DR STRING; 44689.DDBDRAFT_0186395; -.
DR EnsemblProtists; DDB0233403; DDB0233403; DDB_G0285213.
DR GeneID; 8624993; -.
DR KEGG; ddi:DDB_G0285213; -.
DR dictyBase; DDB_G0285213; dhx15.
DR eggNOG; COG1643; -.
DR KO; K12820; -.
DR OMA; TVLQIHA; -.
DR GO; GO:0005681; C:spliceosomal complex; ISS:dictyBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004004; F:ATP-dependent RNA helicase activity; ISS:dictyBase.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006397; P:mRNA processing; ISS:dictyBase.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
DR InterPro; IPR011709; DUF1605.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; FALSE_NEG.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing;
KW mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1 727 Putative pre-mRNA-splicing factor ATP-
FT dependent RNA helicase dhx15.
FT /FTId=PRO_0000330940.
FT DOMAIN 87 251 Helicase ATP-binding.
FT DOMAIN 273 445 Helicase C-terminal.
FT NP_BIND 100 107 ATP (By similarity).
FT MOTIF 198 201 DEAH box.
FT COMPBIAS 24 29 Poly-Glu.
FT COMPBIAS 31 40 Poly-Thr.
SQ SEQUENCE 727 AA; 82858 MW; 153EA093D51E89FB CRC64;
MSKRKHESSD SNKKAMKKQQ NKIEEEEEEI TNTTTTTTTT NNNYNNIKSN LTIDQWIPKK
ETFSKRYYEI LEKRKELPVW KQKEDFIKVI KENQVVILVG ETGSGKTTQI PQFVVDAGLI
RPGKMVGVTQ PRRVAAISVA KRVSEEMDFE LGEEVGYSIR FEELSSARTF MKYLTDGMLL
RESMSDPTLN KYDVIILDEA HERTLSTDIL FGLIKDILKR RKDLKLIVMS ATLEAGKFQK
YFENAPLIKV PGRLHPVEIF YTEEAAKDYL ESAVRTVIDI HTNEGTGDIL VFLTGEEEIE
DTCAKIQRET RERGLPPMKT LPLYSSLPIY QQSKIFDTCK ERKCIVSTNI AETSLTIDGI
VFVVDPGFSK QKTYNPRSRV ESLLVAPISK ASANQRAGRA GRTRPGKCFR LYTEKAFKEL
MIQQTHPEIL RSNLASVVLQ LLKLGVVDLV HFDFMDPPVP DTLIRALEVL HYLGALDDEG
QLTEIGSIMS EFPLDPQLSK MLIVSAERSC SNEILTIAAM LSAPNCFMRP KDNRIEADSA
KKSFDHFDGD HLTMLNVYHS FKKNGEDPTW CYDNFLNHRA IKQADSVRSQ LARILTRFKL
PLVSGDVNSK FYYENIKKCI AAGFFMQVAK CEKKNIYFTL GDEQSVIFHP STGLTRRPEF
CIYNEFVLTS ENYIRTITDV KFDWLLELAP SYFKQKSFPK KTKETIQRAQ RLYSGSSSGS
SSGSNKK
//
