ID CFAD_DICDI Reviewed; 531 AA.
AC Q54TR1;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 01-MAY-2013, entry version 46.
DE RecName: Full=Counting factor associated protein D;
DE Flags: Precursor;
GN Name=cfaD; ORFNames=DDB_G0281605;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP FUNCTION, INTERACTION WITH APRA, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18611962; DOI=10.1242/jcs.026682;
RA Bakthavatsalam D., Brock D.A., Nikravan N.N., Houston K.D.,
RA Hatton R.D., Gomer R.H.;
RT "The secreted Dictyostelium protein CfaD is a chalone.";
RL J. Cell Sci. 121:2473-2480(2008).
RN [3]
RP ERRATUM.
RA Bakthavatsalam D., Brock D.A., Nikravan N.N., Houston K.D.,
RA Hatton R.D., Gomer R.H.;
RL J. Cell Sci. 121:2782-2782(2008).
RN [4]
RP FUNCTION, INTERACTION WITH APRA, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=19187549; DOI=10.1186/1471-2091-10-4;
RA Choe J.M., Bakthavatsalam D., Phillips J.E., Gomer R.H.;
RT "Dictyostelium cells bind a secreted autocrine factor that represses
RT cell proliferation.";
RL BMC Biochem. 10:4-4(2009).
CC -!- FUNCTION: Inhibitor that slows proliferation of secreting cells
CC (also known as chalone). Requires aprA for activity.
CC -!- SUBUNIT: Interacts with aprA.
CC -!- SUBCELLULAR LOCATION: Secreted. Note=Concentrated in subcellular
CC structures, possibly vesicles.
CC -!- DEVELOPMENTAL STAGE: Expressed at highest levels in vegetatively
CC growing cells and declines during development (at protein level).
CC -!- DISRUPTION PHENOTYPE: Cells proliferate more rapidly than wild
CC type cells and exhibit supernumerary centrosomes and a cytokinesis
CC defect. They also produce fewer spores at culmination. Form large
CC fruiting bodies with large spore heads.
CC -!- SIMILARITY: Belongs to the peptidase C1 family.
CC -!- CAUTION: Lacks catalytic activity, even though the active site
CC residues are conserved.
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DR EMBL; AAFI02000042; EAL66564.1; -; Genomic_DNA.
DR RefSeq; XP_640530.1; XM_635438.1.
DR HSSP; P07711; 1CJL.
DR ProteinModelPortal; Q54TR1; -.
DR STRING; 44689.DDB_0229857; -.
DR PRIDE; Q54TR1; -.
DR EnsemblProtists; DDB0229857; DDB0229857; DDB_G0281605.
DR GeneID; 8623140; -.
DR KEGG; ddi:DDB_G0281605; -.
DR dictyBase; DDB_G0281605; cfaD.
DR eggNOG; COG4870; -.
DR InParanoid; Q54TR1; -.
DR OMA; PSAHEHE; -.
DR ProtClustDB; CLSZ2430523; -.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:dictyBase.
DR GO; GO:0005615; C:extracellular space; IDA:dictyBase.
DR GO; GO:0008285; P:negative regulation of cell proliferation; IDA:dictyBase.
DR GO; GO:0031288; P:sorocarp morphogenesis; IMP:dictyBase.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR025660; Pept_his_AS.
DR InterPro; IPR013128; Peptidase_C1A.
DR InterPro; IPR000668; Peptidase_C1A_C.
DR InterPro; IPR013201; Prot_inhib_I29.
DR PANTHER; PTHR12411; PTHR12411; 1.
DR Pfam; PF08246; Inhibitor_I29; 1.
DR Pfam; PF00112; Peptidase_C1; 1.
DR PRINTS; PR00705; PAPAIN.
DR SMART; SM00848; Inhibitor_I29; 1.
DR SMART; SM00645; Pept_C1; 1.
DR PROSITE; PS00640; THIOL_PROTEASE_ASN; FALSE_NEG.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
DR PROSITE; PS00639; THIOL_PROTEASE_HIS; 1.
PE 1: Evidence at protein level;
KW Complete proteome; Disulfide bond; Glycoprotein; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1 18 Potential.
FT CHAIN 19 531 Counting factor associated protein D.
FT /FTId=PRO_0000384376.
FT ACT_SITE 333 333 By similarity.
FT ACT_SITE 475 475 By similarity.
FT ACT_SITE 495 495 By similarity.
FT CARBOHYD 94 94 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 111 111 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 423 423 N-linked (GlcNAc...) (Potential).
FT DISULFID 330 373 By similarity.
FT DISULFID 364 406 By similarity.
FT DISULFID 466 517 By similarity.
SQ SEQUENCE 531 AA; 58638 MW; 248E0C8E1FE13DA5 CRC64;
MNKFILLLSL VTLSCVLAVP QLPAAQQYYM KGSFNIPYFN IVEPIELIYD SVNNRQYISV
YNGMDITINF YNQDNTYNVG PVKYDMVCTT TPGNGSLVNV LPTEPSSWVY NGTSTVNGVQ
VFGYSQKITQ YGRTGFYNFY VDANGVPVQF YMDGVDYVFG SHPDVYVLNF DIYTTDISSY
ESYFDIPVLC NNAKEAPAKE NQFDGLFSSI GDNLLAKEEQ ASNLFKEYKA QYNKEYSSQD
EHDERFINFK AARKIIATHN AKESSYKLGM NHYADLSNKE FNTLVKPKVA RPSVTGADSV
HDDESLRSIP STVDWRNQNC VTPVKDQGIC GSCWTFGSTG SLEGTNCVTN GELVSLSEQQ
LVDCAILTGS QGCGGGFASS AFQYVMEIGS LATESNYPYL MQNGLCRDRT VTPSGVSITG
YVNVTSGSES ALQNAIATTG PVAIAIDASV DDFRYYMSGV YNNPACKNGL DDLDHEVLAI
GYGTYQGQDY FLVKNSWSTN WGMDGYVYMA RNDNNLCGVS SQATYPIPTK N
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