ID GEFX_DICDI Reviewed; 960 AA.
AC Q55EC7;
DT 25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 01-MAY-2013, entry version 56.
DE RecName: Full=RasGEF domain-containing serine/threonine-protein kinase X;
DE EC=2.7.11.1;
DE AltName: Full=Ras guanine nucleotide exchange factor X;
DE AltName: Full=RasGEF domain-containing protein X;
GN Name=gefX; ORFNames=DDB_G0269298;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP DEVELOPMENTAL STAGE.
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple
RT biological roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
CC -!- DEVELOPMENTAL STAGE: Clearly expressed during growth and
CC development. Expression culminates at 8-12 hours of development
CC and is still clearly detectable at 18 hours of development.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr
CC protein kinase family.
CC -!- SIMILARITY: Contains 1 N-terminal Ras-GEF domain.
CC -!- SIMILARITY: Contains 1 protein kinase domain.
CC -!- SIMILARITY: Contains 1 Ras-GEF domain.
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DR EMBL; AAFI02000005; EAL71999.1; -; Genomic_DNA.
DR RefSeq; XP_645854.1; XM_640762.1.
DR HSSP; P15056; 1UWH.
DR ProteinModelPortal; Q55EC7; -.
DR EnsemblProtists; DDB0229854; DDB0229854; DDB_G0269298.
DR GeneID; 8616798; -.
DR KEGG; ddi:DDB_G0269298; -.
DR dictyBase; DDB_G0269298; gefX.
DR eggNOG; COG0515; -.
DR OMA; FDINPEE; -.
DR GO; GO:0005622; C:intracellular; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR011009; Kinase-like_dom.
DR InterPro; IPR000719; Prot_kinase_cat_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom.
DR InterPro; IPR001895; RasGRF_CDC25.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF07714; Pkinase_Tyr; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR PRINTS; PR00109; TYRKINASE.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF56112; Kinase_like; 1.
DR SUPFAM; SSF48366; Ras_GEF; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS00720; RASGEF; FALSE_NEG.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Complete proteome; Guanine-nucleotide releasing factor;
KW Kinase; Nucleotide-binding; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1 960 RasGEF domain-containing
FT serine/threonine-protein kinase X.
FT /FTId=PRO_0000354061.
FT DOMAIN 21 274 Protein kinase.
FT DOMAIN 437 565 N-terminal Ras-GEF.
FT DOMAIN 712 957 Ras-GEF.
FT NP_BIND 27 35 ATP (By similarity).
FT COMPBIAS 282 298 Poly-Ala.
FT COMPBIAS 320 331 Poly-Asn.
FT COMPBIAS 596 604 Poly-Asn.
FT COMPBIAS 607 616 Poly-Asn.
FT COMPBIAS 620 623 Poly-Ser.
FT COMPBIAS 624 652 Poly-Asn.
FT ACT_SITE 140 140 Proton acceptor (By similarity).
FT BINDING 48 48 ATP (By similarity).
SQ SEQUENCE 960 AA; 107769 MW; 0EB63003194DE7AE CRC64;
MAEADPGLPT QAIWDIPFES LEFNEKIGKG SFGSVFRGCY LGLDVAIKKI EKADDPEYLK
YIDREVSMLQ SLRHPFIVNF SGICVHSSGL YIVTEFVSGG DVRQLLKKTP PIGWDKRVSI
AVDLAKAMVF LHAKKIIHRD LKSKNILLDE FQRIRLCDFG FARMSEQTKK SRHMTMCGTE
GWVAPEILLG MSYDTSCDVF SYGVVLAELI TGRKPGVDLW VRSPETCFDI NPEELKQKSI
PGCPSELISV CVECCLYEPL TRPKFDEILS QLKVCQNNLK VATAAAAAAA AAAAAAAAVV
STPTIQTPII QTPNISFSPN NSNNNNNNNN NISNISPDIT TGIQQINLSS SGGSNNSSPS
TPPQGSQLVS LAQSRRNTMS LHRKSMELNL VDGQLSTTPP PTSPIQSRPH KPSDSIWKIA
AKPKHVGYQT LKRKQGPCYA ALTSHITKMI ERATSDYYYD TSYIQDFLLA YRCFAPPQQI
FELLLSRYIA NSPDNFTNDI NGWKKVQRVI QLRVIIFFKR WIDYYPQDFL EEAMEDNLNE
FDKISAQQNS STALLLGTTI SNNELLIDPK LMTELQKKRS ELELLIQINS PSDFINNNNN
NNNNPVNNIN NINNNNSVNS SSSNNNNNNN NNNSNNNNNN NNNNNNNNNN NNGLNIINIA
AANQSKMMLQ NGNNRYSVLV TSNGIGNGEE PYPVSIIPPP TTSEYLDVKD IHSTELARQI
TIINSFYFNR IKAREFIEYI WEKCGEESTT TPYVGTSFVE VVPAENIHKF VRKCNNLARF
VSTEILKQTK LQKRVATIER FIEAAEKCLA NNDYAAVFSI VEPLVDQSIE RLSDTWRNVS
QRNLATFEHL KSIVSKENDH KKYRELLPDA KPPCIPNIHL LLDELSFIET SSPRLLPGGI
VNFFHYRQLS RKILQSQQLQ SHCFRPIPSI QKVLTKPPSE LFDDELIKNN SLKCEPPVSL
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