ID C524A_DICDI Reviewed; 532 AA.
AC Q55EK2;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 27-MAR-2024, entry version 116.
DE RecName: Full=Probable cytochrome P450 524A1;
DE EC=1.14.-.-;
GN Name=cyp524A1; ORFNames=DDB_G0269016;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AAFI02000004; EAL73097.1; -; Genomic_DNA.
DR RefSeq; XP_647018.1; XM_641926.1.
DR AlphaFoldDB; Q55EK2; -.
DR SMR; Q55EK2; -.
DR STRING; 44689.Q55EK2; -.
DR PaxDb; 44689-DDB0233032; -.
DR EnsemblProtists; EAL73097; EAL73097; DDB_G0269016.
DR GeneID; 8616711; -.
DR KEGG; ddi:DDB_G0269016; -.
DR dictyBase; DDB_G0269016; cyp524A1.
DR eggNOG; KOG0157; Eukaryota.
DR HOGENOM; CLU_023517_1_0_1; -.
DR InParanoid; Q55EK2; -.
DR OMA; MVIPSFY; -.
DR PhylomeDB; Q55EK2; -.
DR PRO; PR:Q55EK2; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0016125; P:sterol metabolic process; IBA:GO_Central.
DR CDD; cd11082; CYP61_CYP710; 1.
DR Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR PANTHER; PTHR24286:SF228; C-22 STEROL DESATURASE ERG5; 1.
DR PANTHER; PTHR24286; CYTOCHROME P450 26; 1.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; Cytochrome P450; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 3: Inferred from homology;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..532
FT /note="Probable cytochrome P450 524A1"
FT /id="PRO_0000318841"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 478
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 532 AA; 59871 MW; 5E4D7F8641CB754A CRC64;
MKTPTKYFII FILLAALAVF VSEATSKVGQ QTTTTTQQVK CSGLQCTLNK LIVAVGKFTI
KQILVGIVIV LATIALHQQY VITQKKGSLP GPSFVPPFFG MLFQLIFTPF SFYEKQEKYG
PISWTSIMNK FVLFVTDAEI NRQVFKEENA KLYLSLGAKK ILTEKAIPFI EGAPHRQLRK
QLLPLFTIRA LSSYLPIQES IVDEHIAMWI KNGKADINAR NNCRDLNMAI STGVFVGNNT
PESVRDDIAK NFFVMNEGFL CLPIDLPGTT LRKAINARVR LVEIFTDIIA KSRKRMGDGE
KPQSLIDLWV EHFLNCPEEE RDELSNDTII FTLLSFMFAS QDALTSSLVW TVQLMAEHPD
ILAKVRAEQA SLRPNNEKLD LDTMRQATYT RMVVSEILRF RPPAVMVPHE NIEDIVIGDN
VHVPKGTMIL PSIWSAHFQE GGYSDPYKFD PQRFDSVRKE DVTCAKNSLV FGAGPHFCIG
KELAKNQIEV FLTKLAMSTE WTHNKTPGGD EIIFGPTIFP KDGCNITIKA RN
//
