ID Q57VK5_TRYB2 Unreviewed; 1270 AA.
AC Q57VK5; D6XJD7;
DT 10-MAY-2005, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2005, sequence version 1.
DT 27-MAR-2024, entry version 104.
DE RecName: Full=adenylate cyclase {ECO:0000256|ARBA:ARBA00012201};
DE EC=4.6.1.1 {ECO:0000256|ARBA:ARBA00012201};
DE AltName: Full=ATP pyrophosphate-lyase {ECO:0000256|ARBA:ARBA00032597};
DE AltName: Full=Adenylyl cyclase {ECO:0000256|ARBA:ARBA00032637};
GN Name=Tb07.2F2.270 {ECO:0000313|EMBL:AAZ12670.1};
GN ORFNames=Tb927.7.6060 {ECO:0000313|EMBL:AAX70364.1};
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431 {ECO:0000313|EMBL:AAX70364.1, ECO:0000313|Proteomes:UP000008524};
RN [1] {ECO:0000313|EMBL:AAZ12670.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=927/4 GUTat10.1 {ECO:0000313|EMBL:AAZ12670.1};
RX PubMed=16020724; DOI=10.1126/science.1112181;
RA El-Sayed N.M., Myler P.J., Blandin G., Berriman M., Crabtree J.,
RA Aggarwal G., Caler E., Renauld H., Worthey E.A., Hertz-Fowler C.,
RA Ghedin E., Peacock C., Bartholomeu D.C., Haas B.J., Tran A.N.,
RA Wortman J.R., Alsmark U.C., Angiuoli S., Anupama A., Badger J.,
RA Bringaud F., Cadag E., Carlton J.M., Cerqueira G.C., Creasy T.,
RA Delcher A.L., Djikeng A., Embley T.M., Hauser C., Ivens A.C.,
RA Kummerfeld S.K., Pereira-Leal J.B., Nilsson D., Peterson J., Salzberg S.L.,
RA Shallom J., Silva J.C., Sundaram J., Westenberger S., White O.,
RA Melville S.E., Donelson J.E., Andersson B., Stuart K.D., Hall N.;
RT "Comparative genomics of trypanosomatid parasitic protozoa.";
RL Science 309:404-409(2005).
RN [2] {ECO:0000313|EMBL:AAZ12670.1, ECO:0000313|Proteomes:UP000008524}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1 {ECO:0000313|EMBL:AAZ12670.1,
RC ECO:0000313|Proteomes:UP000008524};
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [3] {ECO:0000313|EMBL:AAX70364.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=GUTat10.1 {ECO:0000313|EMBL:AAX70364.1};
RA Ghedin E., Blandin G., Bartholomeu D., Caler E., Haas B., Hannick L.,
RA Shallom J., Hou L., Djikeng A., Feldblyum T., Hostetler J., Johnson J.,
RA Jones K., Koo H.L., Larkin C., Pai G., Peterson J., Khalak H.G.,
RA Salzberg S., Simpson A.J., Tallon L., Van Aken S., Wanless D., White O.,
RA Wortman J., Fraser C.M., El-Sayed N.M.A.;
RT ".";
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:AAZ12670.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=927/4 GUTat10.1 {ECO:0000313|EMBL:AAZ12670.1};
RA Ghedin E., Blandin G., Bartholomeu D., Caler E., Haas B., Hannick L.,
RA Shallom J., Hou L., Djikeng A., Feldblyum T., Hostetler J., Johnson J.,
RA Jones K., Koo H.L., Larkin C., Pai G., Peterson J., Khalak H.G.,
RA Salzberg S., Simpson A.J., Tallon L., Van Aken S., Wanless D., White O.,
RA Wortman J., Fraser C.M., El-Sayed N.M.A.;
RT "Sequencing, closure, and annotation of Trypanosoma brucei chromosomes 2
RT through 8.";
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Could act as a receptor for an unknown ligand.
CC {ECO:0000256|ARBA:ARBA00002708}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP = 3',5'-cyclic AMP + diphosphate; Xref=Rhea:RHEA:15389,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58165; EC=4.6.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001593};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the adenylyl cyclase class-3 family.
CC {ECO:0000256|ARBA:ARBA00005381}.
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DR EMBL; AC159439; AAX70364.1; -; Genomic_DNA.
DR EMBL; CP000070; AAZ12670.1; -; Genomic_DNA.
DR RefSeq; XP_846229.1; XM_841136.1.
DR AlphaFoldDB; Q57VK5; -.
DR STRING; 185431.Q57VK5; -.
DR PaxDb; 5691-AAZ12670; -.
DR GeneID; 3658820; -.
DR KEGG; tbr:Tb927.7.6060; -.
DR VEuPathDB; TriTrypDB:Tb927.7.6060; -.
DR eggNOG; KOG0618; Eukaryota.
DR InParanoid; Q57VK5; -.
DR OrthoDB; 119841at2759; -.
DR Proteomes; UP000008524; Chromosome 7.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004016; F:adenylate cyclase activity; ISM:GeneDB.
DR GO; GO:0006171; P:cAMP biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0035556; P:intracellular signal transduction; ISM:GeneDB.
DR CDD; cd07556; Nucleotidyl_cyc_III; 1.
DR Gene3D; 3.40.50.2300; -; 2.
DR Gene3D; 3.30.70.1230; Nucleotide cyclase; 1.
DR InterPro; IPR001054; A/G_cyclase.
DR InterPro; IPR028081; Leu-bd.
DR InterPro; IPR029787; Nucleotide_cyclase.
DR InterPro; IPR028082; Peripla_BP_I.
DR PANTHER; PTHR43081; ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED; 1.
DR PANTHER; PTHR43081:SF1; PH-SENSITIVE ADENYLATE CYCLASE RV1264; 1.
DR Pfam; PF00211; Guanylate_cyc; 1.
DR Pfam; PF13458; Peripla_BP_6; 1.
DR SMART; SM00044; CYCc; 1.
DR SUPFAM; SSF55073; Nucleotide cyclase; 1.
DR SUPFAM; SSF53822; Periplasmic binding protein-like I; 1.
DR PROSITE; PS50125; GUANYLATE_CYCLASE_2; 1.
PE 3: Inferred from homology;
KW cAMP biosynthesis {ECO:0000256|ARBA:ARBA00022998};
KW Lyase {ECO:0000313|EMBL:AAX70364.1}; Membrane {ECO:0000256|SAM:Phobius};
KW Receptor {ECO:0000256|ARBA:ARBA00023170, ECO:0000313|EMBL:AAX70364.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000008524};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 842..869
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 886..1040
FT /note="Guanylate cyclase"
FT /evidence="ECO:0000259|PROSITE:PS50125"
SQ SEQUENCE 1270 AA; 139530 MW; AC5D50C0F357975C CRC64;
MPHRAVWGPR GVPCGTVVQL FLLAVILQVM QAPMVLSWNW RGSVTVEVFS PPPESVVSNG
TVKALNTGVL ASFHARGNIT GTGVTVSFSA PATSEQNVNE ALEQKLKGFS DAEKILVVLG
PLGDKTVLES LDLLTKNEVI GFAPYTGSGA VRGWRPNLYF ISVDPLAETL ALLRYALAHL
RVRRLGFMYL KGVSFGDTEY QLVVQAMARM GYGLCGTFAL KSSTEASASS EDFQREWDQF
AATNPQAVIL YVYPVDDGKK ALLKFLEDKR TSGACILSPF ALQYFVVETL NATYGRGGGY
SHGEVITTGT NPLAKDNEYQ AIQRFQKEMK AYLASHSGAS LYGRSDRHLT DDDDDGELMV
YGWIIGEMLS RALSSREWLK NRSTFKKSLY NQRRYVIDDL VYGDYGGDCP DNVAALGASC
QCNQGGKAVY MKRLVEGFRL ESVKSGFMAL GTSRCDTDGS RLHAPLAAVA TFMDNSQLSL
NAAEEWHGGA SLLVGTGDLG DYDRFFLHRI NTSSGTIWKR LKREEEEKLV TAVMGVTDEA
LLSTSTFVVV DPVLLVPRLR TPNKRVIYLS ATLEQQLFVI AKYLGEKGSI GVHSVISSSE
SNRIEKVVNM SLVRFYQEPQ SLVILNATVS VTGYLPREGD VFVIGLKQDD VEVIAKHLSA
NPKLFVYVPF TEVALYYQTF VRVFRDKGPG SSSSNRLLFA TNLPHWADGN PSSDTVKKFH
NAMKDKSKWT PLSLMGFAAG NLLRTVLPQL KKVDADTLSN LFFSETYLRA DDMRYGPYGT
EGCGKVEVGL WNDCVTNYGA TGIAVWSMAR ALDPSVPPIA EPISQLLQYE DRDAGRLTGA
RLIGVCVGAV LFFLLLVVVI VVVLCRYFAD ARDNANAPKE PTDPVTLIFT DIESSTAQWA
AHPEMMPDAV ATHHRLIRAL IMHYRCYEVK TVGDSFMIAC RSPSAAVRLA CDLQHNFLHH
NWGTAALDES YREFELQRAE EEEGYTPPTA HLDREVYGRL WRGLRVRVGI HTGLCDIRYD
EVTKGYDYYG RTANMAARTE SVANGGQVLM TRATYMSLSV VEREQFDVTA LGPVTLRGVP
NPVEMYQLNP IPGRTFAALR LDRDGPGLDD ECSSPSLSEV SSLCAGLTDS ARQVAISLDS
LLGVFAPAQR QNLLLPLCER WRVSLLRKNS GWSEAYCRDT VHRIAAKVGH ITDHKGKRTS
MDLSTTSDSV CVLQPSLAGI ANLRSPLLSR SNYPGDNGDL ASVGIVASSD ASEALSRRST
VEVTSMKGDL
//
