ID ATG21_CANAL Reviewed; 529 AA.
AC Q5AI22; A0A1D8PCZ9;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 27-MAR-2024, entry version 102.
DE RecName: Full=Autophagy-related protein 21;
GN Name=ATG21; OrderedLocusNames=CAALFM_C103330CA;
GN ORFNames=CaO19.10548, CaO19.3030;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- FUNCTION: Required for cytoplasm to vacuole transport (Cvt) vesicles
CC formation and mitophagy. Involved in binding of
CC phosphatidylethanolamine to ATG8 and in recruitment of ATG8 and ATG5 to
CC the pre-autophagosomal structure. Protects ATG8 from ARG4-mediated
CC cleavage (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}. Vacuole membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Vacuolar
CC and perivacuolar punctate structures. {ECO:0000250}.
CC -!- DOMAIN: Contains a beta-propeller domain involved in specific binding
CC to phosphatidylinositol 3,5-bisphosphate (PIP2). {ECO:0000250}.
CC -!- DOMAIN: The L/FRRG motif is essential for the cytoplasm to vacuole
CC transport (Cvt) pathway and for the recruitment of ATG8 and ATG16 to
CC the PAS in nutrient-rich medium and in both its recruitment to and
CC dissociation from the PAS under starvation conditions.
CC {ECO:0000250|UniProtKB:Q02887}.
CC -!- SIMILARITY: Belongs to the WD repeat PROPPIN family. {ECO:0000305}.
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DR EMBL; CP017623; AOW26014.1; -; Genomic_DNA.
DR RefSeq; XP_721528.1; XM_716435.2.
DR AlphaFoldDB; Q5AI22; -.
DR SMR; Q5AI22; -.
DR STRING; 237561.Q5AI22; -.
DR EnsemblFungi; C1_03330C_A-T; C1_03330C_A-T-p1; C1_03330C_A.
DR GeneID; 3636859; -.
DR KEGG; cal:CAALFM_C103330CA; -.
DR CGD; CAL0000201683; orf19.10548.
DR VEuPathDB; FungiDB:C1_03330C_A; -.
DR eggNOG; KOG2110; Eukaryota.
DR HOGENOM; CLU_025895_5_2_1; -.
DR InParanoid; Q5AI22; -.
DR OrthoDB; 391429at2759; -.
DR PRO; PR:Q5AI22; -.
DR Proteomes; UP000000559; Chromosome 1.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0019898; C:extrinsic component of membrane; IBA:GO_Central.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0034045; C:phagophore assembly site membrane; IBA:GO_Central.
DR GO; GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IBA:GO_Central.
DR GO; GO:0044804; P:nucleophagy; IBA:GO_Central.
DR GO; GO:0034497; P:protein localization to phagophore assembly site; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR048720; PROPPIN.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR11227:SF17; WD REPEAT DOMAIN PHOSPHOINOSITIDE-INTERACTING PROTEIN 2; 1.
DR PANTHER; PTHR11227; WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED; 1.
DR Pfam; PF21032; PROPPIN; 2.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
PE 3: Inferred from homology;
KW Autophagy; Cytoplasm; Membrane; Protein transport; Reference proteome;
KW Repeat; Transport; Vacuole; WD repeat.
FT CHAIN 1..529
FT /note="Autophagy-related protein 21"
FT /id="PRO_0000050876"
FT REPEAT 271..311
FT /note="WD 1"
FT REPEAT 321..361
FT /note="WD 2"
FT REGION 49..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 362..388
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 318..322
FT /note="L/FRRG motif"
FT /evidence="ECO:0000250|UniProtKB:Q02887"
FT COMPBIAS 362..387
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 529 AA; 60019 MW; A31F3C9C491028EB CRC64;
MVTINDLSFN PDYSSISVST SDGFKIFNCE PFGEFYSSQE SPLRKSISNS LEDSAGCQNP
THSKTDSQDT PARFPTAFLK MLFSTSLTIV VPQTQDNLGN RLLKIYNLKQ NLKICELNFP
SSIIDIKLNR KRLLVVLDTG QLYIYDLSCV RLLKILQLSF NEHDGDQKFI GDLSADDSSW
LIIPVQSTNN QTDLLNAETG SQPSTPKLTP SDSVINTGSY SQYLEFTRNS SLSNLKKKNK
LITLEDIKND SEGWVVVYDT INLAPVVIFE AHHSTIARIC ISHRDNKVAT ASIKGTIIRI
FDLKEFEGKV KVHKVKNLRR GHNLVKVNSL SFHNDNHILG CGSESNTIHL FKIHEEESDI
CTNENSEDRT NHNSDYEDSD GDTSKSSEDL NENLANLLIS KPLDPVPMET EDKLSSSWFV
KTKKLINNQY TSSIIKKLPY KDYFENLIWE PPQRSFAYIK LPEYAPHNEK DPRSNKVEIG
FNNDLVFIAS YHTGNFYQYQ IPKQRGPVSS INEDDKREEC SLISQFNLI
//
