ID Q5GNE1_VIBVL Unreviewed; 309 AA.
AC Q5GNE1;
DT 01-MAR-2005, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2005, sequence version 1.
DT 13-SEP-2023, entry version 73.
DE RecName: Full=DNA-directed RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00015972};
DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418};
DE AltName: Full=RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00033070};
DE AltName: Full=Transcriptase subunit alpha {ECO:0000256|ARBA:ARBA00032524};
DE Flags: Fragment;
GN Name=rpoA {ECO:0000313|EMBL:CAH57466.1};
OS Vibrio vulnificus.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=672 {ECO:0000313|EMBL:CAH57466.1};
RN [1] {ECO:0000313|EMBL:CAH57466.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=R-15063 {ECO:0000313|EMBL:CAH57467.1}, and Type strain: LMG
RC 13545 {ECO:0000313|EMBL:CAH57466.1};
RX PubMed=16151093; DOI=10.1128/AEM.71.9.5107-5115.2005;
RA Thompson F.L., Gevers D., Thompson C.C., Dawyndt P., Naser S., Hoste B.,
RA Munn C.B., Swings J.;
RT "Phylogeny and molecular identification of vibrios on the basis of
RT multilocus sequence analysis.";
RL Appl. Environ. Microbiol. 71:5107-5115(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550};
CC -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC {ECO:0000256|ARBA:ARBA00007123}.
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DR EMBL; AJ842737; CAH57466.1; -; Genomic_DNA.
DR EMBL; AJ842738; CAH57467.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5GNE1; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd06928; RNAP_alpha_NTD; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR HAMAP; MF_00059; RNApol_bact_RpoA; 1.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR011773; DNA-dir_RpoA.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR011260; RNAP_asu_C.
DR InterPro; IPR036643; RNApol_insert_sf.
DR NCBIfam; TIGR02027; rpoA; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF03118; RNA_pol_A_CTD; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF47789; C-terminal domain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 16..228
FT /note="DNA-directed RNA polymerase RpoA/D/Rpb3-type"
FT /evidence="ECO:0000259|SMART:SM00662"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:CAH57466.1"
FT NON_TER 309
FT /evidence="ECO:0000313|EMBL:CAH57466.1"
SQ SEQUENCE 309 AA; 34116 MW; 9EA9C835430FFED1 CRC64;
FLKPRLVDIE QISSTHAKVT LEPLERGFGH TLGNALRRIL LSSMPGCAVT EVEIEGVLHE
YSTKEGVQED ILEILLNLKG LAVRVAEGKD EVFITLNKSG SGPVVAGDIT HDGDVEIANP
EHVICHLTDD NAEIAMRIKV ERGRGYVPAS ARIHTEEDER PIGRLLVDAT YSPVDKIAYA
VEAARVEQRT DLDKLVIDME TNGTLEPEEA IRRAATILAE QLDAFVDLRD VRVPEEKEEK
PEFDPILLRP VDDLELTVRS ANCLKAEAIH YIGDLVQRTE VELLKTPNLG KKSLTEIKDV
LASRGLSLG
//
