ID Q5GNK4_9VIBR Unreviewed; 309 AA.
AC Q5GNK4;
DT 01-MAR-2005, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2005, sequence version 1.
DT 13-SEP-2023, entry version 70.
DE RecName: Full=DNA-directed RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00015972};
DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418};
DE AltName: Full=RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00033070};
DE AltName: Full=Transcriptase subunit alpha {ECO:0000256|ARBA:ARBA00032524};
DE Flags: Fragment;
GN Name=rpoA {ECO:0000313|EMBL:CAH57403.1};
OS Vibrio pacinii.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=170674 {ECO:0000313|EMBL:CAH57403.1};
RN [1] {ECO:0000313|EMBL:CAH57403.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=LMG 13245 {ECO:0000313|EMBL:CAH57402.1}, R-15016
RC {ECO:0000313|EMBL:CAH57404.1}, and Type strain: LMG 19999
RC {ECO:0000313|EMBL:CAH57403.1};
RX PubMed=16151093; DOI=10.1128/AEM.71.9.5107-5115.2005;
RA Thompson F.L., Gevers D., Thompson C.C., Dawyndt P., Naser S., Hoste B.,
RA Munn C.B., Swings J.;
RT "Phylogeny and molecular identification of vibrios on the basis of
RT multilocus sequence analysis.";
RL Appl. Environ. Microbiol. 71:5107-5115(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550};
CC -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC {ECO:0000256|ARBA:ARBA00007123}.
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DR EMBL; AJ842673; CAH57402.1; -; Genomic_DNA.
DR EMBL; AJ842674; CAH57403.1; -; Genomic_DNA.
DR EMBL; AJ842675; CAH57404.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5GNK4; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd06928; RNAP_alpha_NTD; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR HAMAP; MF_00059; RNApol_bact_RpoA; 1.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR011773; DNA-dir_RpoA.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR011260; RNAP_asu_C.
DR InterPro; IPR036643; RNApol_insert_sf.
DR NCBIfam; TIGR02027; rpoA; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF03118; RNA_pol_A_CTD; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF47789; C-terminal domain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 16..228
FT /note="DNA-directed RNA polymerase RpoA/D/Rpb3-type"
FT /evidence="ECO:0000259|SMART:SM00662"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:CAH57403.1"
FT NON_TER 309
FT /evidence="ECO:0000313|EMBL:CAH57403.1"
SQ SEQUENCE 309 AA; 34098 MW; 5FCC1C18BD439A89 CRC64;
FLKPRLVDIE QVSTTHAKVT LEPLERGFGH TLGNALRRIL LSSMPGCAVT EVEIEGVLHE
YSTKEGVQED ILEILLNLKG LAVHVAEGKD EVFITLNKSG SGPVVAGDIT HDGDVEIANP
EHVICHLTND NAEIAMRIKV ERGRGYVPAS ARIHTEEDER PIGRLLVDAT YSPVDKIAYS
VEAARVEQRT DLDKLVIDME TNGTLDPEEA IRRAATILAE QLDAFVDLRD VRVPEEKEEK
PEFDPILLRP VDDLELTVRS ANCLKAEAIH YIGDLVQRTE VELLKTPNLG KKSLTEIKDV
LASRGLSLG
//
