ID TFE_THEKO Reviewed; 185 AA.
AC Q5JDD5;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 27-MAR-2024, entry version 106.
DE RecName: Full=Transcription factor E {ECO:0000255|HAMAP-Rule:MF_01909};
DE Short=TFE {ECO:0000255|HAMAP-Rule:MF_01909};
DE AltName: Full=TFIIE subunit alpha homolog {ECO:0000255|HAMAP-Rule:MF_01909};
DE AltName: Full=Transcription initiation factor TFIIE {ECO:0000255|HAMAP-Rule:MF_01909};
GN Name=tfe {ECO:0000255|HAMAP-Rule:MF_01909}; OrderedLocusNames=TK2024;
OS Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS (Pyrococcus kodakaraensis (strain KOD1)).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=69014;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX PubMed=15710748; DOI=10.1101/gr.3003105;
RA Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL Genome Res. 15:352-363(2005).
CC -!- FUNCTION: Transcription factor that plays a role in the activation of
CC archaeal genes transcribed by RNA polymerase. Facilitates transcription
CC initiation by enhancing TATA-box recognition by TATA-box-binding
CC protein (Tbp), and transcription factor B (Tfb) and RNA polymerase
CC recruitment. Not absolutely required for transcription in vitro, but
CC particularly important in cases where Tbp or Tfb function is not
CC optimal. It dynamically alters the nucleic acid-binding properties of
CC RNA polymerases by stabilizing the initiation complex and destabilizing
CC elongation complexes. Seems to translocate with the RNA polymerase
CC following initiation and acts by binding to the non template strand of
CC the transcription bubble in elongation complexes. {ECO:0000255|HAMAP-
CC Rule:MF_01909}.
CC -!- SUBUNIT: Monomer. Interaction with RNA polymerase subunits RpoF and
CC RpoE is necessary for Tfe stimulatory transcription activity. Able to
CC interact with Tbp and RNA polymerase in the absence of DNA promoter.
CC Interacts both with the preinitiation and elongation complexes.
CC {ECO:0000255|HAMAP-Rule:MF_01909}.
CC -!- DOMAIN: The winged helix domain is involved in binding to DNA in the
CC preinitiation complex. {ECO:0000255|HAMAP-Rule:MF_01909}.
CC -!- SIMILARITY: Belongs to the TFE family. {ECO:0000255|HAMAP-
CC Rule:MF_01909}.
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DR EMBL; AP006878; BAD86213.1; -; Genomic_DNA.
DR RefSeq; WP_011250974.1; NC_006624.1.
DR PDB; 6KF4; EM; 3.97 A; G=1-185.
DR PDB; 6KF9; EM; 3.79 A; G=1-185.
DR PDBsum; 6KF4; -.
DR PDBsum; 6KF9; -.
DR AlphaFoldDB; Q5JDD5; -.
DR SMR; Q5JDD5; -.
DR STRING; 69014.TK2024; -.
DR EnsemblBacteria; BAD86213; BAD86213; TK2024.
DR GeneID; 78448559; -.
DR KEGG; tko:TK2024; -.
DR PATRIC; fig|69014.16.peg.1978; -.
DR eggNOG; arCOG04270; Archaea.
DR HOGENOM; CLU_100097_0_0_2; -.
DR InParanoid; Q5JDD5; -.
DR OrthoDB; 5935at2157; -.
DR PhylomeDB; Q5JDD5; -.
DR Proteomes; UP000000536; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR GO; GO:0001113; P:transcription open complex formation at RNA polymerase II promoter; IBA:GO_Central.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR HAMAP; MF_01909; TFE_arch; 1.
DR InterPro; IPR016481; TF_E_archaea.
DR InterPro; IPR039997; TFE.
DR InterPro; IPR017919; TFIIE/TFIIEa_HTH.
DR InterPro; IPR002853; TFIIE_asu.
DR InterPro; IPR024550; TFIIEa/SarR/Rpc3_HTH_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR NCBIfam; TIGR00373; transcription factor E; 1.
DR PANTHER; PTHR13097:SF7; GENERAL TRANSCRIPTION FACTOR IIE, POLYPEPTIDE 1, ALPHA; 1.
DR PANTHER; PTHR13097; TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT; 1.
DR Pfam; PF02002; TFIIE_alpha; 1.
DR PIRSF; PIRSF006373; TF_E_archaea; 1.
DR SMART; SM00531; TFIIE; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51344; HTH_TFE_IIE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..185
FT /note="Transcription factor E"
FT /id="PRO_0000326621"
FT DOMAIN 5..88
FT /note="HTH TFE/IIEalpha-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01909"
SQ SEQUENCE 185 AA; 22050 MW; F83E9D6789A9783A CRC64;
MAKRKNKELL EIAQEIGGEE AVEVVKALEK MKEATDEELA EATGIRVNTV RRILYMLNDE
GLADFKRIRD PETGWYYYYW RLETKKLPEI IRSRKMAELK KLKEMLEEET SEIYYWCGTE
GHPKLTFDEA MEYEFQCPIC GKMLMQYDNT HIIEELKRRI EELEIELGLK KKPRKTSKKK
KSRSE
//
