ID Q5LM96_RUEPO Unreviewed; 376 AA.
AC Q5LM96;
DT 01-FEB-2005, integrated into UniProtKB/TrEMBL.
DT 01-FEB-2005, sequence version 1.
DT 27-MAR-2024, entry version 109.
DE SubName: Full=Muconate cycloisomerase I {ECO:0000313|EMBL:AAV96890.1};
DE EC=5.5.1.1 {ECO:0000313|EMBL:AAV96890.1};
GN Name=catB {ECO:0000313|EMBL:AAV96890.1};
GN OrderedLocusNames=SPO3667 {ECO:0000313|EMBL:AAV96890.1};
OS Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter
OS pomeroyi).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Ruegeria.
OX NCBI_TaxID=246200 {ECO:0000313|EMBL:AAV96890.1, ECO:0000313|Proteomes:UP000001023};
RN [1] {ECO:0000313|EMBL:AAV96890.1, ECO:0000313|Proteomes:UP000001023}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700808 / DSM 15171 / DSS-3
RC {ECO:0000313|Proteomes:UP000001023};
RX PubMed=15602564; DOI=10.1038/nature03170;
RA Moran M.A., Buchan A., Gonzalez J.M., Heidelberg J.F., Whitman W.B.,
RA Kiene R.P., Henriksen J.R., King G.M., Belas R., Fuqua C., Brinkac L.,
RA Lewis M., Johri S., Weaver B., Pai G., Eisen J.A., Rahe E., Sheldon W.M.,
RA Ye W., Miller T.R., Carlton J., Rasko D.A., Paulsen I.T., Ren Q.,
RA Daugherty S.C., Deboy R.T., Dodson R.J., Durkin A.S., Madupu R.,
RA Nelson W.C., Sullivan S.A., Rosovitz M.J., Haft D.H., Selengut J., Ward N.;
RT "Genome sequence of Silicibacter pomeroyi reveals adaptations to the marine
RT environment.";
RL Nature 432:910-913(2004).
RN [2] {ECO:0007829|PDB:3I6E}
RP X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 3-376 IN COMPLEX WITH MAGNESIUM.
RA Fedorov A.A., Fedorov E.V., Sauder J.M., Burley S.K., Gerlt J.A.,
RA Almo S.C.;
RT "Crystal structure of muconate lactonizing enzyme from Ruegeria pomeroyi.";
RL Submitted (JUL-2009) to the PDB data bank.
RN [3] {ECO:0000313|EMBL:AAV96890.1, ECO:0000313|Proteomes:UP000001023}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 700808 / DSM 15171 / DSS-3
RC {ECO:0000313|Proteomes:UP000001023};
RX PubMed=25780504; DOI=10.1186/1944-3277-9-11;
RA Rivers A.R., Smith C.B., Moran M.A.;
RT "An updated genome annotation for the model marine bacterium Ruegeria
RT pomeroyi DSS-3.";
RL Stand. Genomic Sci. 9:11-11(2014).
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- PATHWAY: Aromatic compound metabolism. {ECO:0000256|ARBA:ARBA00005211}.
CC -!- SIMILARITY: Belongs to the mandelate racemase/muconate lactonizing
CC enzyme family. {ECO:0000256|ARBA:ARBA00008031}.
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DR EMBL; CP000031; AAV96890.1; -; Genomic_DNA.
DR PDB; 3I6E; X-ray; 1.70 A; A/B/C/D/E/F/G/H=3-376.
DR PDBsum; 3I6E; -.
DR AlphaFoldDB; Q5LM96; -.
DR SMR; Q5LM96; -.
DR STRING; 246200.SPO3667; -.
DR PaxDb; 246200-SPO3667; -.
DR KEGG; sil:SPO3667; -.
DR eggNOG; COG4948; Bacteria.
DR HOGENOM; CLU_030273_4_5_5; -.
DR EvolutionaryTrace; Q5LM96; -.
DR Proteomes; UP000001023; Chromosome.
DR GO; GO:0018850; F:chloromuconate cycloisomerase activity; IEA:InterPro.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProt.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0018849; F:muconate cycloisomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0009063; P:amino acid catabolic process; IEA:InterPro.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR CDD; cd03318; MLE; 1.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR InterPro; IPR013370; Chloromuconate_cycloisomerase.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR018110; Mandel_Rmase/mucon_lact_enz_CS.
DR InterPro; IPR013342; Mandelate_racemase_C.
DR InterPro; IPR013341; Mandelate_racemase_N_dom.
DR PANTHER; PTHR48073:SF2; O-SUCCINYLBENZOATE SYNTHASE; 1.
DR PANTHER; PTHR48073; O-SUCCINYLBENZOATE SYNTHASE-RELATED; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR Pfam; PF02746; MR_MLE_N; 1.
DR SFLD; SFLDS00001; Enolase; 1.
DR SFLD; SFLDG00180; muconate_cycloisomerase; 1.
DR SMART; SM00922; MR_MLE; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
DR PROSITE; PS00908; MR_MLE_1; 1.
DR PROSITE; PS00909; MR_MLE_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:3I6E};
KW Aromatic hydrocarbons catabolism {ECO:0000256|ARBA:ARBA00022797};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:AAV96890.1};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0007829|PDB:3I6E};
KW Reference proteome {ECO:0000313|Proteomes:UP000001023}.
FT DOMAIN 148..244
FT /note="Mandelate racemase/muconate lactonizing enzyme C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00922"
FT BINDING 197
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:3I6E"
FT BINDING 223
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:3I6E"
FT BINDING 248
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:3I6E"
SQ SEQUENCE 376 AA; 40825 MW; C7EF33473252E443 CRC64;
MMGDLEQKII AMDLWHLALP VVSARDHGIG RVEGSCEIVV LRLVAEGGAE GFGEASPWAV
FTGTPEASYA ALDRYLRPLV IGRRVGDRVA IMDEAARAVA HCTEAKAALD SALLDLAGRI
SNLPVWALLG GKCRDTIPLS CSIANPDFDA DIALMERLRA DGVGLIKLKT GFRDHAFDIM
RLELIARDFP EFRVRVDYNQ GLEIDEAVPR VLDVAQFQPD FIEQPVRAHH FELMARLRGL
TDVPLLADES VYGPEDMVRA AHEGICDGVS IKIMKSGGLT RAQTVARIAA AHGLMAYGGD
MFEAGLAHLA GTHMIAATPE ITLGCEFYQA SYFLNEDILE TPFRVEAGQV IVPDGPGLGA
RADPEKLEHY AVRRSG
//
