ID Q5P524_AROAE Unreviewed; 543 AA.
AC Q5P524;
DT 04-JAN-2005, integrated into UniProtKB/TrEMBL.
DT 04-JAN-2005, sequence version 1.
DT 27-MAR-2024, entry version 104.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN Name=hsdM {ECO:0000313|EMBL:CAI07588.1};
GN ORFNames=ebA2607 {ECO:0000313|EMBL:CAI07588.1};
OS Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
OC Rhodocyclaceae; Aromatoleum.
OX NCBI_TaxID=76114 {ECO:0000313|EMBL:CAI07588.1, ECO:0000313|Proteomes:UP000006552};
RN [1] {ECO:0000313|EMBL:CAI07588.1, ECO:0000313|Proteomes:UP000006552}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EbN1 {ECO:0000313|EMBL:CAI07588.1,
RC ECO:0000313|Proteomes:UP000006552};
RX PubMed=15551059; DOI=10.1007/s00203-004-0742-9;
RA Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F.,
RA Reinhardt R.;
RT "The genome sequence of an anaerobic aromatic-degrading denitrifying
RT bacterium, strain EbN1.";
RL Arch. Microbiol. 183:27-36(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CR555306; CAI07588.1; -; Genomic_DNA.
DR RefSeq; WP_011237306.1; NC_006513.1.
DR AlphaFoldDB; Q5P524; -.
DR STRING; 76114.ebA2607; -.
DR REBASE; 10644; M.AarEBORF2607P.
DR KEGG; eba:ebA2607; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_013049_0_2_4; -.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000006552; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000006552};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 9..172
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 183..505
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 502..536
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 543 AA; 61669 MW; 1C4101D75D63AD63 CRC64;
MTEQDQKQLG KTLWAIADQL RGSMNADDFR DYMLSFLFLR YLSDNYEQAA KKELGADYPD
VAPGVMEQTD STTPLQIWYE ENADDVAEFE KQMRRKVHYV IEPDYLWGSI VHLAKTESSK
LLDTLQKGFK YIEERSFQSN FQGLFSEINL ASDKLGRKYE DRNDKLCSII SELARGMSLF
STDTDTLGDA YEYLIGQFAA GSGKKAGEFY TPQQISDILS AIVTLDSQEP KEGPRKKLEN
VFDFACGSGS LLLNVRHRMK KAGGTIGKIY GQEYNVTTYN LARMNMLLHG VKDTEFEIYH
GDTLANTWDS LRETNPAKKP QFDAVVANPP FSYRWDLGDA MSEDMRFKNH GVAPKSAADF
AFLLHGLHYL KDDGVMAIIL PHGVLFRGGA EERIRRKLLI DGHIDTVVGL PANLFYSTGI
PVCILVLKKC KKPDDVLFIN AAEHFEKGKR QNQLSDEHIQ RIIDTYQQRP SDIPRYARRV
GMEEIEKNDF NLNISRYVST AVAEEEVDLK AVHQQLVDIE KQIVKAKDKH NAFLKELGLP
SLP
//
