ID GHC1_PONAB Reviewed; 323 AA.
AC Q5RD81;
DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 22-FEB-2023, entry version 74.
DE RecName: Full=Mitochondrial glutamate carrier 1;
DE Short=GC-1;
DE AltName: Full=Glutamate/H(+) symporter 1;
DE AltName: Full=Solute carrier family 25 member 22;
GN Name=SLC25A22;
OS Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pongo.
OX NCBI_TaxID=9601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain cortex;
RG The German cDNA consortium;
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mitochondrial glutamate/H(+) symporter. Responsible for the
CC transport of glutamate from the cytosol into the mitochondrial matrix
CC with the concomitant import of a proton (By similarity). Plays a role
CC in the control of glucose-stimulated insulin secretion (By similarity).
CC {ECO:0000250|UniProtKB:A0A0G2K5L2, ECO:0000250|UniProtKB:Q9H936}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(in) + L-glutamate(in) = H(+)(out) + L-glutamate(out);
CC Xref=Rhea:RHEA:70955, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985;
CC Evidence={ECO:0000250|UniProtKB:Q9H936};
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:A0A0G2K5L2}; Multi-pass membrane protein
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; CR858035; CAH90276.1; -; mRNA.
DR RefSeq; NP_001125123.1; NM_001131651.1.
DR AlphaFoldDB; Q5RD81; -.
DR SMR; Q5RD81; -.
DR STRING; 9601.ENSPPYP00000003354; -.
DR GeneID; 100172007; -.
DR KEGG; pon:100172007; -.
DR CTD; 79751; -.
DR eggNOG; KOG0750; Eukaryota.
DR InParanoid; Q5RD81; -.
DR OrthoDB; 1220009at2759; -.
DR Proteomes; UP000001595; Unplaced.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005280; F:amino acid:proton symporter activity; ISS:UniProtKB.
DR GO; GO:0050796; P:regulation of insulin secretion; ISS:UniProtKB.
DR Gene3D; 1.50.40.10; Mitochondrial carrier domain; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR PANTHER; PTHR45678; MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED; 1.
DR PANTHER; PTHR45678:SF3; MITOCHONDRIAL GLUTAMATE CARRIER 1; 1.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; Mitochondrial carrier; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..323
FT /note="Mitochondrial glutamate carrier 1"
FT /id="PRO_0000231653"
FT TRANSMEM 12..32
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 6..93
FT /note="Solcar 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 101..214
FT /note="Solcar 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 223..312
FT /note="Solcar 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
SQ SEQUENCE 323 AA; 34494 MW; A0E6590AB6002793 CRC64;
MADKQISLPA KLINGGIAGL IGVTCVFPID LAKTRLQNQQ NGQRMYTSMS DCLIKTIRSE
GYFGMYRGAA VNLTLVTPEK AIKLAANDFF RHQLSKDGQK LTLLKEMLAG CGAGTCQVIV
TTPMEMLKIQ LQDAGRIAAQ RKILAAQGQL SAQGGAQPSV EAPAAPRPTA IQLTRDLLRS
RGIAGLYKGL GATLLRDVPL SVVYFPLFAN LNQLGRPASE EKSPFYVSFL AGCVAGSAAA
VAVNPCDVVK TRLQSLQRGV NEDTYSGILD CARKILRHEG PSAFLKGAYC RALVIAPLFG
IAQVVYFLGI AESLLGLLQD PQA
//
