ID THOC6_MOUSE Reviewed; 341 AA.
AC Q5U4D9;
DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 27-MAR-2024, entry version 145.
DE RecName: Full=THO complex subunit 6 homolog;
DE AltName: Full=WD repeat-containing protein 58;
GN Name=Thoc6; Synonyms=Wdr58;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Fetal brain, and Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Acts as a component of the THO subcomplex of the TREX complex
CC which is thought to couple mRNA transcription, processing and nuclear
CC export, and which specifically associates with spliced mRNA and not
CC with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a
CC transcription-independent mechanism, binds to mRNA upstream of the
CC exon-junction complex (EJC) and is recruited in a splicing- and cap-
CC dependent manner to a region near the 5' end of the mRNA where it
CC functions in mRNA export to the cytoplasm via the TAP/NFX1
CC pathway.Plays a role in apoptosis negative control involved in brain
CC development (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the THO complex, which is composed of THOC1,
CC THOC2, THOC3, THOC5, THOC6 and THOC7; together with at least
CC ALYREF/THOC4, DDX39B, SARNP/CIP29 and CHTOP, THO forms the
CC transcription/export (TREX) complex which seems to have a dynamic
CC structure involving ATP-dependent remodeling. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Nucleus speckle
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat THOC6 family. {ECO:0000305}.
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DR EMBL; BC085131; AAH85131.1; -; mRNA.
DR EMBL; BC089551; AAH89551.1; -; mRNA.
DR CCDS; CCDS28454.1; -.
DR RefSeq; NP_001008425.1; NM_001008425.1.
DR RefSeq; XP_006524607.1; XM_006524544.2.
DR AlphaFoldDB; Q5U4D9; -.
DR SMR; Q5U4D9; -.
DR BioGRID; 239749; 2.
DR IntAct; Q5U4D9; 2.
DR STRING; 10090.ENSMUSP00000038137; -.
DR iPTMnet; Q5U4D9; -.
DR PhosphoSitePlus; Q5U4D9; -.
DR REPRODUCTION-2DPAGE; IPI00123949; -.
DR EPD; Q5U4D9; -.
DR MaxQB; Q5U4D9; -.
DR PaxDb; 10090-ENSMUSP00000038137; -.
DR PeptideAtlas; Q5U4D9; -.
DR ProteomicsDB; 259021; -.
DR Pumba; Q5U4D9; -.
DR Antibodypedia; 23992; 180 antibodies from 26 providers.
DR DNASU; 386612; -.
DR Ensembl; ENSMUST00000047436.11; ENSMUSP00000038137.5; ENSMUSG00000041319.15.
DR Ensembl; ENSMUST00000095579.11; ENSMUSP00000093239.5; ENSMUSG00000041319.15.
DR Ensembl; ENSMUST00000115490.9; ENSMUSP00000111153.3; ENSMUSG00000041319.15.
DR GeneID; 386612; -.
DR KEGG; mmu:386612; -.
DR UCSC; uc008ass.1; mouse.
DR AGR; MGI:2677480; -.
DR CTD; 79228; -.
DR MGI; MGI:2677480; Thoc6.
DR VEuPathDB; HostDB:ENSMUSG00000041319; -.
DR eggNOG; KOG0649; Eukaryota.
DR GeneTree; ENSGT00390000015278; -.
DR HOGENOM; CLU_060667_0_0_1; -.
DR InParanoid; Q5U4D9; -.
DR OMA; HLRSMEC; -.
DR OrthoDB; 8092at2759; -.
DR PhylomeDB; Q5U4D9; -.
DR TreeFam; TF324760; -.
DR Reactome; R-MMU-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-MMU-72187; mRNA 3'-end processing.
DR Reactome; R-MMU-73856; RNA Polymerase II Transcription Termination.
DR BioGRID-ORCS; 386612; 16 hits in 80 CRISPR screens.
DR ChiTaRS; Thoc6; mouse.
DR PRO; PR:Q5U4D9; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q5U4D9; Protein.
DR Bgee; ENSMUSG00000041319; Expressed in dorsal pancreas and 212 other cell types or tissues.
DR ExpressionAtlas; Q5U4D9; baseline and differential.
DR Genevisible; Q5U4D9; MM.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:Ensembl.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000347; C:THO complex; ISO:MGI.
DR GO; GO:0000445; C:THO complex part of transcription export complex; ISO:MGI.
DR GO; GO:0000346; C:transcription export complex; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0007417; P:central nervous system development; ISS:UniProtKB.
DR GO; GO:0006406; P:mRNA export from nucleus; ISO:MGI.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR GO; GO:0046784; P:viral mRNA export from host cell nucleus; ISO:MGI.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR042626; THOC6.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR44411; THO COMPLEX SUBUNIT 6 HOMOLOG; 1.
DR PANTHER; PTHR44411:SF1; THO COMPLEX SUBUNIT 6 HOMOLOG; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Developmental protein; mRNA processing; mRNA splicing;
KW mRNA transport; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW RNA-binding; Transport; WD repeat.
FT CHAIN 1..341
FT /note="THO complex subunit 6 homolog"
FT /id="PRO_0000233159"
FT REPEAT 22..61
FT /note="WD 1"
FT REPEAT 74..112
FT /note="WD 2"
FT REPEAT 124..165
FT /note="WD 3"
FT REPEAT 166..205
FT /note="WD 4"
FT REPEAT 215..254
FT /note="WD 5"
FT REPEAT 256..293
FT /note="WD 6"
FT REPEAT 295..339
FT /note="WD 7"
FT MOD_RES 180
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86W42"
SQ SEQUENCE 341 AA; 37315 MW; D5B32EC62A7E6D40 CRC64;
MEHAAPLAVP LGQAEVFQAL QRLHMTIFSQ SVSPCGKFLA AGNNYGQIAI FSLSAALSSE
AKEESKKPVV VFHAHDGPVY SMVSTDRHLL SAGDGEVKGW LWAEILKKGC KELWRRQPPY
RTSLEVPEIN ALLLVPKENS LILAGGDCQL HSMDLETGAF TRALRGHTDY IHCLALRERS
PEVLSGGEDG AVRLWDLRIA KEVQTIEVYK HEECSRPHNG RWIGCLATDS DWMVCGGGPA
LTLWHLRSST PTTVFPIRAP QKHVTFYQDL ILSAGQGCCV NHWQLSGELK AQVPGSSPGL
LSLSLNQQPA APECKVLTAS GNSCRVDVFT NLGYRAFSLS F
//
