ID Q5UJG0_9VIRU Unreviewed; 3945 AA.
AC Q5UJG0;
DT 07-DEC-2004, integrated into UniProtKB/TrEMBL.
DT 04-JAN-2005, sequence version 2.
DT 27-MAR-2024, entry version 75.
DE RecName: Full=RNA-directed RNA polymerase L {ECO:0000256|ARBA:ARBA00018602};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494};
DE EC=3.4.19.12 {ECO:0000256|ARBA:ARBA00012759};
DE AltName: Full=Large structural protein {ECO:0000256|ARBA:ARBA00030285};
DE AltName: Full=Replicase {ECO:0000256|ARBA:ARBA00031012};
DE AltName: Full=Transcriptase {ECO:0000256|ARBA:ARBA00030436};
OS Orthonairovirus haemorrhagiae.
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Ellioviricetes; Bunyavirales; Nairoviridae; Orthonairovirus.
OX NCBI_TaxID=3052518 {ECO:0000313|EMBL:AAV39276.2};
RN [1] {ECO:0000313|EMBL:AAV39276.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=TADJ/HU8966 {ECO:0000313|EMBL:AAV39276.2};
RX PubMed=16195783; DOI=10.1007/s00705-005-0648-0;
RA Meissner J.D., Seregin S.S., Seregin S.V., Yakimenko N.V.,
RA Vyshemirskii O.I., Netesov S.V., Petrov V.S.;
RT "Complete L segment coding-region sequences of Crimean Congo hemorrhagic
RT fever virus strains from the Russian Federation and Tajikistan.";
RL Arch. Virol. 151:465-475(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC residue protein attached to proteins as an intracellular targeting
CC signal).; EC=3.4.19.12; Evidence={ECO:0000256|ARBA:ARBA00000707};
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DR EMBL; AY720893; AAV39276.2; -; Genomic_RNA.
DR MEROPS; C87.001; -.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd21880; OTU_RNAP_L_virus; 1.
DR Gene3D; 3.90.70.80; -; 1.
DR InterPro; IPR049605; L_OTU.
DR InterPro; IPR003323; OTU_dom.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR015843; RNA-dir_pol_nairovirus.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR007322; RNA_pol_bunyavir.
DR PANTHER; PTHR13312; HIV-INDUCED PROTEIN-7-LIKE PROTEASE; 1.
DR PANTHER; PTHR13312:SF0; UBIQUITIN THIOESTERASE OTU1; 1.
DR Pfam; PF04196; Bunya_RdRp; 1.
DR Pfam; PF02338; OTU; 1.
DR PIRSF; PIRSF036900; RdRPol_NRV; 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR PROSITE; PS50802; OTU; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 4: Predicted;
KW Nucleotidyltransferase {ECO:0000313|EMBL:AAV39276.2};
KW RNA-directed RNA polymerase {ECO:0000313|EMBL:AAV39276.2};
KW Transferase {ECO:0000313|EMBL:AAV39276.2}.
FT DOMAIN 29..158
FT /note="OTU"
FT /evidence="ECO:0000259|PROSITE:PS50802"
FT DOMAIN 2342..2551
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT REGION 2716..2742
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 40
FT /note="For ubiquitin thioesterase activity"
FT /evidence="ECO:0000256|PIRSR:PIRSR036900-50"
FT ACT_SITE 151
FT /note="For ubiquitin thioesterase activity"
FT /evidence="ECO:0000256|PIRSR:PIRSR036900-50"
SQ SEQUENCE 3945 AA; 447758 MW; 9A15CA008ED8D784 CRC64;
MDFLRNLDWT QVIAGQYVTN PRFNISDYFE IVRQPGDGNC FYHSIAELTM PNKTDHSYHN
IKHLTEVAAR KYYQEEPEAK LIGLSLEDYI KRMLSDNEWG STLEASMLAK EMGVTIIIWT
VTASDEVEAG IKFGDGDVFT AVNLLHSGQT HFDALRLLPQ FEADTRETLS LVDKVIAVDQ
LTSSSSDELQ DYEDLALALT SVEEPYRRSS LDEVTLSKKQ AEILRQKASQ LSKLVNKSQN
IPTRVGRVLD CMFNCKLCVE ISADTLILRP ESKERIGEVM SLRQLGHKLL TRDKQIKQEF
SRMKLYVTKD LLDHLDVGGL LRAAFPGTGI ERHMQLLHSE MILDICTVSL GIMLSTFLYG
SNNKNKKKFI TNCLLSTALS GKKVYKVLGN LGNELLYKAP RKALATVCSA LFGKQINKLQ
NCFRTISPVS LLALRNLDFD CLSVQDYNGM IENMSKLDNT DVEFNHREIA DLNQLTSRLI
TLRKEKDADL LKQWFPEGDL TRRSTRNVAN AEEFIISEFF KKKDIMKFIS TSGRAMSAGK
IGNVLSYAHN LYLSKSSLNM TSEDISQLLI EIKRLYALQE DSEVEPIAII CDGIESNMKQ
LFAILPPDCA KECEVLFDDI RNSPTHSTAW KHALRLKGTA YEGLFANCYG WQYIPEDIKP
SLTMLIQTLF PDKFEDFLDR TQLHPEFRDL TPDFSLTQKV HFKRNQIPSV ENVQISIDAT
LPESVEAVPV TERKMFPLPE TPLSEVHSIE RIMENFTRLM HGGKPSTKGK GKEPAEQDNY
QNAIEHESSS IMAFKDYGER GIVEENHMRL SEEDQLETRQ LLLVEVGFQT DIDGKIRTDH
KKWKDILKLL ELLGIKCSFI ACADCSSTPP DRWWITEDRV RVLKNSVSFL FNKLSRNSPT
EVTDIVVGAI STQKVRSYLK AGTATKTPVS TKDVLETWER MKEHIVNRPT GLTLPASLEQ
AMRKGLVEGV VISKEGSDSC INMLKENLDR ITDEFERTKF KHELTQNIAT SEKMLLSWLS
EDIKSSRCTE CLSSIKRTVD ETTNLSEKIE LLAYNLQLTS HCSNCHPNGV NISNVSNVCK
RCPKIEVVSH CENKGFEDSN ECLTDLDRLV RLTLPGKTEK ERRVKRNVEY LIKLMMSMSG
IDCIKYPTGQ LITHGRVSAK LNDGNLKDRS DDDQRLAEKV DAVRKELSET KLKDYSTYAR
GVISNSLKNL SKQGKSKCSV PRSWLEKILF DLKVPTKDEE VLINIRNSLK ARSEFVRNND
KLLIRSKEEL KKCFDVQSFK LMKNKQPMPF QVDCILFKEV AAECMKRYIG TPYEGIIDTL
VSLINVLTRF TWFQEVILYG KICETFLRCC TEFNRSGVKL VKVRHCDINL SVKLPSNKKE
NMLCCIYSSN MELLQGPFYL NRRQAVLGSS YLYIVITLYI QVLQQHRCLE VINSVNEKTL
QDIENHSLTL LEDAFKELTS ALEGRFEESY KIRTTRCKTS GNFLNRSSRD HFISIVSGLN
LVYGFLIKDN LLANSQQQNK QLQMLRFGML AGLSRLVCPN ELGKKFSTSC RRIEDNIARL
YLQTSIYCSV RDVEDNIKHW KQRDLCPEVT IPCFTVYGTF VNSDRQLIFD IYNVHIYNKE
MDNFDEGCIS VLEETAERHM LWELDLMNSL CSDEKKDART ARLLLGCPNV RKAANKEGKK
LLKLNSDTST DTQSVASEVS DRRSYSSSKS RIRSIFGRYN SQKKPFELRS GLEVFNDPFN
DYQQAITDIC QFSEYTPNKE SVLKDCLQII RKNPSHTMGS FELIQAISEF GMSKFPPENI
DKARRDPKNW VSISEVTETT SIVASPKTHM MLKDCFKIIL GTENKKIVKM LRGKLKKLGA
ISTNIEIGKR DCLDLLSTVD GLTDQQKENI VNGIFEPSKL SFYHWKELVK KNIDEVLLTE
DGNLIFCWLK TISSSVKGSL KKRLKFMNIH SPELMPENCL FSSEEFNELI RLKKLLLNEQ
QDEQELKQDL LISSWIKCIT ACKDFASIND KVQKFIYHLS EELYDIRLQH LELSKLKQEH
PSVSFTKEEV LIKRLEKNFL KQHNLEIMET VNLIFFAALS APWCLHYKAL ESYLVRHPEI
LDCGSKEDCR LPLLDLSVSK LLVCLYQKDD EELASGLGLK LGFLVKYAVT LFTSNGEPFS
LSLNDGGLDL DLHKTTDEKL LHQTKIVFAK IGLSGNGYDF IWTTQMIANS NFNVCKRLTG
RSTGERLPRS VRSKVIYEMV KLVGETGMAI LQQLAFAQAL NYEHRFYAVL APKAQLGGAR
DLLVQETGTK VMHATTEMFS RNLLKTTSDD GLTNPHLKET ILNVGLDCLT NMRNLDGRPI
NEGSNLVNFY KVICISGDNT KWGPIHCCSF FSGMMQQVLK NVPDWCSFYK LTFVKNLCRQ
VEIPAGSIKK ILNVLRYKLC SKGGVEQHSE EDLRKLLVDN LDSWDGNDTV KFLVTTYISK
GLMALNSYNH MGQGIHHATS SVLTSLAAVL FEELAIFYLK KSLPQTTVHV EHAGSSDDYA
KCIVVTGIIS KELYSQYDET FWKHACRLKN FTAAVQRCCQ MKDSAKTLVS DCFLEFYSEF
MMGYRVTPAV IKFMFTGLIN SSVTSPQSLM QACQVSSQQA MYNSVPLVTN TAFTLLRQQI
FFNHVEDFIR RYGILTLGTL SPFGRLFVPT YSGLVSSAVA LEDAEVIARA AQTLHMNSVS
IQSSSLTTLD SLGRSRTSST VEDSSSVSDT TVASHDSGLS SSSFSFELNR PLSETELQFI
KALNSLKSTQ ACEVIQNRIT GLYCNSNEGP LDRHNVIYSS RMADSCDWLR DGKRRGNLEL
ANRIQSVLCV LIAGYYRSFG GEGTEKQVKA SLNRDDNKII EDPMIQLIPE KLRRELERLG
VSRMEVDELM PSISPDDTLA QLVAKKLISL NVSTEEYSAE VSRLKQTLTA RNVLHGLAGG
IKELSLPIYT IFMKSYFFKD NVFLSLTDRW STKHSTNYRD SCGKQLTGRI ITKYTHWLDT
FLSCSVSINR HTAVKEPSLF NPNIRCVNLI TFEDGLRELS VIQSHLKVFE SEFTNLNLQF
SDPNRQKLKI VESRPAESEL EANRAVIVKT KLFSATEQVR LSNNPAVVMG YLLDESAISE
VKPTKVDFSN LLKDRFKIMQ FFPSVFTLIK MLTDESSDSE KNGLSPDLQQ VARYSNHLTL
LSRMIQQAKP TVTVFYMLKG NLMNTEPTVA ELVSYGIKEG RFYRLSDTGI DASTYSVKYW
KILHCISAIG CLPLSQADKS SLLMSFLNWR VNMDIRASDC PLSNHEASIL SEFDGQVIAN
ILASELSSVK RDSEREGLTD LLDYLNSPTE LLKKKPYLGT TCKFNTWGDS NRSGKFTYSS
RSGESIGIFI AGKLHIHLSS ESIALLCETE RQVLSWMSKR RTEVITKEQH QLFLSLLPQS
HECLQKHKDG SALSVIPDSS NPRLLKFVPL KKGLAVVKIK KQILTVKKQV VLDAESEPRL
QWGHGCLSIV YDETDTQTTY HENLLKVKQL VDCSTDRKKL LPQSVFSDSK VVLSRIKFKT
ELLLNSLTLL HCFLKHAPSD AIMEVESKSS LLHKYLKSGG VRQRNTEVLF REKLNRIVIK
DNLEQGVEEE IEFCNNLTKT VSENPLPLSC WSEVQNYIED IGFNNVLVNI DRNTVKSELL
WKFTLDTNVS TTSTIKDVRT LVSYVSTETI PKFLLAFLLY EEVLMNLINQ CKAVKELINS
TGLSDLELES LLTLCAFYFQ NECSKRDGPR CSFAALLSLV HEDWQKIGKN ILVRANNELG
DVSLKVNIIL VPLKDMSKSK PERVVIARRS LNHALSLMFL DEMSLPELKS LSVNCRMGNF
EGQECFEFTI LKDNSTRLDY NKLIDHCVDM EKKRDAVRAV EDLVLMLTGR AVKPSTVTPT
AHEDEQCQEQ ISLDDLMASD TTADLPDKEA EALRTGNLGF NWDSD
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