ID Q5UU95_LACHE Unreviewed; 628 AA.
AC Q5UU95;
DT 07-DEC-2004, integrated into UniProtKB/TrEMBL.
DT 07-DEC-2004, sequence version 1.
DT 27-MAR-2024, entry version 101.
DE RecName: Full=beta-galactosidase {ECO:0000256|ARBA:ARBA00012756};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756};
GN Name=lacL {ECO:0000313|EMBL:AAU11509.1};
GN ORFNames=DM470_10445 {ECO:0000313|EMBL:PXZ11737.1}, DQL95_03650
GN {ECO:0000313|EMBL:AZA19621.1}, Lh22155_03555
GN {ECO:0000313|EMBL:AUI75899.1};
OS Lactobacillus helveticus (Lactobacillus suntoryeus).
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=1587 {ECO:0000313|EMBL:AAU11509.1};
RN [1] {ECO:0000313|EMBL:AAU11509.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DPC4571 {ECO:0000313|EMBL:AAU11509.1};
RX PubMed=15746373; DOI=10.1128/AEM.71.3.1655-1658.2005;
RA Callanan M.J., Beresford T.P., Ross R.P.;
RT "Genetic diversity in the lactose operons of Lactobacillus helveticus
RT strains and its relationship to the role of these strains as commercial
RT starter cultures.";
RL Appl. Environ. Microbiol. 71:1655-1658(2005).
RN [2] {ECO:0000313|Proteomes:UP000234533}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FAM22155 {ECO:0000313|Proteomes:UP000234533};
RA Ahrens C., Schmid M.;
RT "Genome sequence of Lactobacillus helveticus FAM22155.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:AUI75899.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=FAM22155 {ECO:0000313|EMBL:AUI75899.1};
RX PubMed=29441050;
RA Schmid M., Muri J., Melidis D., Varadarajan A.R., Somerville V., Wicki A.,
RA Moser A., Bourqui M., Wenzel C., Eugster-Meier E., Frey J.E., Irmler S.,
RA Ahrens C.H.;
RT "Comparative Genomics of Completely Sequenced Lactobacillus helveticus
RT Genomes Provides Insights into Strain-Specific Genes and Resolves
RT Metagenomics Data Down to the Strain Level.";
RL Front. Microbiol. 9:63-63(2018).
RN [4] {ECO:0000313|EMBL:PXZ11737.1, ECO:0000313|Proteomes:UP000247635}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FAM19188 {ECO:0000313|EMBL:PXZ11737.1,
RC ECO:0000313|Proteomes:UP000247635};
RA Moser A.I., Irmler S., Wuethrich D., Bruggmann R., Eugster-Meier E.,
RA Berthoud H.;
RT "Cell lysis in Lactobacillus helveticus.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:AZA19621.1, ECO:0000313|Proteomes:UP000281589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NWC_2_4 {ECO:0000313|EMBL:AZA19621.1};
RA Somerville V.;
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401, ECO:0000256|RuleBase:RU361154}.
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DR EMBL; AY616518; AAU11509.1; -; Genomic_DNA.
DR EMBL; CP015498; AUI75899.1; -; Genomic_DNA.
DR EMBL; CP031018; AZA19621.1; -; Genomic_DNA.
DR EMBL; QJOO01000121; PXZ11737.1; -; Genomic_DNA.
DR RefSeq; WP_012212097.1; NZ_WCHF01000108.1.
DR AlphaFoldDB; Q5UU95; -.
DR OMA; QEVKYYY; -.
DR Proteomes; UP000234533; Chromosome.
DR Proteomes; UP000247635; Unassembled WGS sequence.
DR Proteomes; UP000281589; Chromosome.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR023232; Glyco_hydro_2_AS.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR023230; Glyco_hydro_2_CS.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361154};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361154}.
FT DOMAIN 43..227
FT /note="Glycosyl hydrolases family 2 sugar binding"
FT /evidence="ECO:0000259|Pfam:PF02837"
FT DOMAIN 229..331
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 338..625
FT /note="Glycoside hydrolase family 2 catalytic"
FT /evidence="ECO:0000259|Pfam:PF02836"
SQ SEQUENCE 628 AA; 73578 MW; F5C53CF9A900EB0B CRC64;
MQANINWLDN PEVFRVNQLP AHSDHPFFRD YREWQKQHSS YQQSLNGKWK FHFSANPMDR
PQDFYQRDFD SSNFDSIPVP SEIELSNYTQ NQYINVLFPW EGKIFRRPAY ALDPNDHEEG
SFSKGADNTV GSYLKRFDLS SALIGKDVHI KFEGVEQAMY VWLNGHFVGY AEDSFTPSEF
DLTPYIQEKD NLLAVEVFKH STASWLEDQD MFRFSGIFRS VELLGIPATH LMDMDLKPRV
ADNYQDGIFN LKLHFIGKKA GSFHLLVKDI KGHTLLEKNE DIKENVQINN EKFENVHLWN
NHDPYLYQLL IEVYDEQQNL LELIPFQFGF RRIEISPEKV VLLNGKRLII NGVNRHEWDA
KRGRSITMSD MTTDINTFKE NNINAVRTCH YPNQIPWYYL CDQNGIYVMA ENNLESHGTW
QKMGEIEPSD NVPGSIPQWK EAVIDRARNN YETFKNHTSI LFWSLGNESY AGDNIIAMNE
FYKSHDDTRL VHYEGVVHRP ELKDKISDVE SCMYLPPKKV EEYLQNDPPK PFMECEYMHD
MGNSNGGMDS YIKLLDKYPQ YFGGFIWDFI DQALLVHDEI SGHDVLRYGG DFDDRHSDYE
FSGDGLMFAD RKPKPAMQEV RYYYGLHK
//
