ID Q5W8I0_COMTE Unreviewed; 252 AA.
AC Q5W8I0;
DT 07-DEC-2004, integrated into UniProtKB/TrEMBL.
DT 07-DEC-2004, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE SubName: Full=Enoyl-CoA hydratase {ECO:0000313|EMBL:BAD66683.1, ECO:0000313|EMBL:GEQ76109.1};
GN Name=scdY {ECO:0000313|EMBL:GEQ76109.1};
GN ORFNames=CTTA_3114 {ECO:0000313|EMBL:GEQ76109.1};
OS Comamonas testosteroni (Pseudomonas testosteroni).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Comamonas.
OX NCBI_TaxID=285 {ECO:0000313|EMBL:BAD66683.1};
RN [1] {ECO:0000313|EMBL:BAP91339.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1};
RX PubMed=11739769;
RA Horinouchi M., Hayashi T., Taguchi K., Arai H., Kudo T.;
RT "Meta-cleavage enzyme gene tesB is necessary for testosterone degradation
RT in Comamonas testosteroni TA441.";
RL Microbiology 147:3367-3375(2001).
RN [2] {ECO:0000313|EMBL:BAP91339.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1};
RX PubMed=12676694; DOI=10.1128/AEM.69.4.2139-2152.2003;
RA Horinouchi M., Hayashi T., Koshino H., Yamamoto T., Kudo T.;
RT "Gene encoding the hydrolase for the product of the meta-cleavage reaction
RT in testosterone degradation by Comamonas testosteroni.";
RL Appl. Environ. Microbiol. 69:2139-2152(2003).
RN [3] {ECO:0000313|EMBL:BAP91339.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1};
RX PubMed=12902225; DOI=10.1128/AEM.69.8.4421-4430.2003;
RA Horinouchi M., Hayashi T., Yamamoto T., Kudo T.;
RT "A new bacterial steroid degradation gene cluster in Comamonas testosteroni
RT TA441 which consists of aromatic-compound degradation genes for seco-
RT steroids and 3-ketosteroid dehydrogenase genes.";
RL Appl. Environ. Microbiol. 69:4421-4430(2003).
RN [4] {ECO:0000313|EMBL:BAD66683.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1}, and TA441
RC {ECO:0000313|EMBL:BAD66683.1};
RX PubMed=15474469; DOI=10.1016/j.bbrc.2004.09.096;
RA Horinouchi M., Kurita T., Yamamoto T., Hatori E., Hayashi T., Kudo T.;
RT "Steroid degradation gene cluster of Comamonas testosteroni consisting of
RT 18 putative genes from meta-cleavage enzyme gene tesB to regulator gene
RT tesR.";
RL Biochem. Biophys. Res. Commun. 324:597-604(2004).
RN [5] {ECO:0000313|EMBL:BAP91339.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1};
RX PubMed=15555908; DOI=10.1016/j.jsbmb.2004.09.002;
RA Horinouchi M., Hayashi T., Kudo T.;
RT "The genes encoding the hydroxylase of 3-hydroxy-9,10-
RT secoandrosta-1,3,5(10)-triene-9,17-dione in steroid degradation in
RT Comamonas testosteroni TA441.";
RL J. Steroid Biochem. Mol. Biol. 92:143-154(2004).
RN [6] {ECO:0000313|EMBL:BAP91339.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1};
RX PubMed=16151114; DOI=10.1128/AEM.71.9.5275-5281.2005;
RA Horinouchi M., Hayashi T., Koshino H., Kurita T., Kudo T.;
RT "Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-
RT hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related
RT enzymes involved in testosterone degradation in Comamonas testosteroni
RT TA441.";
RL Appl. Environ. Microbiol. 71:5275-5281(2005).
RN [7] {ECO:0000313|EMBL:BAP91339.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1};
RX PubMed=16891113; DOI=10.1016/j.jsbmb.2006.06.006;
RA Horinouchi M., Hayashi T., Koshino H., Kudo T.;
RT "ORF18-disrupted mutant of Comamonas testosteroni TA441 accumulates
RT significant amounts of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid
RT and its derivatives after incubation with steroids.";
RL J. Steroid Biochem. Mol. Biol. 101:78-84(2006).
RN [8] {ECO:0000313|EMBL:BAP91339.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1};
RX PubMed=18539741; DOI=10.1128/JB.01080-07;
RA Horinouchi M., Hayashi T., Koshino H., Malon M., Yamamoto T., Kudo T.;
RT "Identification of genes involved in inversion of stereochemistry of a C-12
RT hydroxyl group in the catabolism of cholic acid by Comamonas testosteroni
RT TA441.";
RL J. Bacteriol. 190:5545-5554(2008).
RN [9] {ECO:0000313|EMBL:BAP91339.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1};
RX PubMed=20554032; DOI=10.1016/j.jsbmb.2010.06.002;
RA Horinouchi M., Kurita T., Hayashi T., Kudo T.;
RT "Steroid degradation genes in Comamonas testosteroni TA441: Isolation of
RT genes encoding a ?4(5)-isomerase and 3?- and 3?-dehydrogenases and evidence
RT for a 100 kb steroid degradation gene hot spot.";
RL J. Steroid Biochem. Mol. Biol. 122:253-263(2010).
RN [10] {ECO:0000313|EMBL:BAP91339.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1};
RX PubMed=21056662; DOI=10.1016/j.jsbmb.2010.10.008;
RA Horinouchi M., Hayashi T., Kudo T.;
RT "Steroid degradation in Comamonas testosteroni.";
RL J. Steroid Biochem. Mol. Biol. 129:4-14(2012).
RN [11] {ECO:0000313|EMBL:BAP91339.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1};
RX PubMed=25092028; DOI=10.1128/JB.01878-14;
RA Horinouchi M., Hayashi T., Koshino H., Malon M., Hirota H., Kudo T.;
RT "Identification of 9?-hydroxy-17-oxo-1,2,3,4,10,19-hexanorandrostan-5-oic
RT acid in steroid degradation by Comamonas testosteroni TA441 and its
RT conversion to the corresponding 6-en-5-oyl coenzyme A (CoA) involving open
RT reading frame 28 (ORF28)- and ORF30-encoded acyl-CoA dehydrogenases.";
RL J. Bacteriol. 196:3598-3608(2014).
RN [12] {ECO:0000313|EMBL:BAP91339.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T441 {ECO:0000313|EMBL:BAP91339.1};
RX PubMed=24810629; DOI=10.1016/j.jsbmb.2014.04.014;
RA Horinouchi M., Hayashi T., Koshino H., Malon M., Hirota H., Kudo T.;
RT "Identification of 9?-hydroxy-17-oxo-1,2,3,4,10,19-hexanorandrost-6-en-5-
RT oic acid and ?-oxidation products of the C-17 side chain in cholic acid
RT degradation by Comamonas testosteroni TA441.";
RL J. Steroid Biochem. Mol. Biol. 143:306-322(2014).
RN [13] {ECO:0000313|EMBL:GEQ76109.1, ECO:0000313|Proteomes:UP000323105}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TA441 {ECO:0000313|EMBL:GEQ76109.1,
RC ECO:0000313|Proteomes:UP000323105};
RA Arai H., Ishii M.;
RT "Draft Genome Sequence of Comamonas testosteroni TA441, a Bacterium That
RT Has a Cryptic Phenol Degradation Gene Cluster.";
RL Microbiol. Resour. Announc. 8:e00946-19(2019).
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DR EMBL; AB186487; BAD66683.1; -; Genomic_DNA.
DR EMBL; LC010134; BAP91339.1; -; Genomic_DNA.
DR EMBL; BKBW01000005; GEQ76109.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5W8I0; -.
DR Proteomes; UP000323105; Unassembled WGS sequence.
DR CDD; cd06558; crotonase-like; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR PANTHER; PTHR11941:SF54; ENOYL-COA HYDRATASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR11941; ENOYL-COA HYDRATASE-RELATED; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
PE 4: Predicted;
SQ SEQUENCE 252 AA; 27294 MW; 7E21AA9F7B2887B5 CRC64;
MSTQQFHSNI HDNGVAELVI DRAPVNALNA AGWNGLAREI QALGDRPEVR VIVIRAENRG
FCAGVDIKEL AEDDKLILDV NAGNYATFKA VHLNKVPVIT AVHGFVLGGG IGICGASDIV
IAAEDATFGL PEVDRGAMGG AAHLQRMFGV QKTRYLFFTG EMIGAAEALR LGAIERVVPR
EQLRDVAMEI ANKIAAKSPA MIRIAKEALT GIEDGNLEDK YRWEQGFTLQ AYMSPDSAET
RSAFVEKRDA KF
//
