ID Q5YCX9_ECOLX Unreviewed; 286 AA.
AC Q5YCX9;
DT 10-MAY-2005, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2005, sequence version 1.
DT 27-MAR-2024, entry version 100.
DE RecName: Full=Beta-lactamase {ECO:0000256|ARBA:ARBA00012865, ECO:0000256|RuleBase:RU361140};
DE EC=3.5.2.6 {ECO:0000256|ARBA:ARBA00012865, ECO:0000256|RuleBase:RU361140};
GN Name=blaSHV-2 {ECO:0000313|EMBL:ADG60243.1};
GN ORFNames=RCS27_P0112 {ECO:0000313|EMBL:SPD96359.1};
OS Escherichia coli.
OG Plasmid RCS27_p {ECO:0000313|EMBL:SPD96359.1}, and
OG Plasmid pHR1 {ECO:0000313|EMBL:AAT97112.1}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562 {ECO:0000313|EMBL:AAT97112.1};
RN [1] {ECO:0000313|EMBL:AAT97112.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=E128-3 {ECO:0000313|EMBL:AAT97112.1};
RC PLASMID=pHR1 {ECO:0000313|EMBL:AAT97112.1};
RA Garza-Ramos U., Silva J.;
RT "Multi-copy of blaSHV-derived genes encoded in conserved fragments in high
RT molecular weight plasmids from clinical isolates of Enterobacteriaceae.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ABY82053.1}
RP NUCLEOTIDE SEQUENCE.
RA Tian G., Wang H., Zhao Y., Ye M.;
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:ABY82053.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=19272004; DOI=10.1089/fpd.2008.0164;
RA Tian G.B., Wang H.N., Zou L.K., Tang J.N., Zhao Y.W., Ye M.Y., Tang J.Y.,
RA Zhang Y., Zhang A.Y., Yang X., Xu C.W., Fu Y.J.;
RT "Detection of CTX-M-15, CTX-M-22, and SHV-2 extended-spectrum beta-
RT lactamases (ESBLs) in Escherichia coli fecal-sample isolates from pig farms
RT in China.";
RL Foodborne Pathog. Dis. 6:297-304(2009).
RN [4] {ECO:0000313|EMBL:ADG60243.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=RS343 {ECO:0000313|EMBL:ADG60243.1};
RA Xu L., Shabir S., Bodah T., McMurray C., Hardy K., Hawkey P., Nye K.;
RT "Regional survey of CTX-M-type ESBL among Enterobacteriaceae: long-term
RT persistence of O25b-ST131 clone of the CTX-M-15-producing Escherichia coli
RT strain.";
RL Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:SPD96359.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=525 {ECO:0000313|EMBL:SPD96359.1};
RC PLASMID=RCS27_p {ECO:0000313|EMBL:SPD96359.1};
RA Cohen D.B., Kent A.D.;
RL Submitted (FEB-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC ChEBI:CHEBI:140347; EC=3.5.2.6;
CC Evidence={ECO:0000256|ARBA:ARBA00001526,
CC ECO:0000256|RuleBase:RU361140};
CC -!- SIMILARITY: Belongs to the class-A beta-lactamase family.
CC {ECO:0000256|ARBA:ARBA00009009, ECO:0000256|RuleBase:RU361140}.
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DR EMBL; AY570957; AAT97112.1; -; Genomic_DNA.
DR EMBL; EU376967; ABY82053.1; -; Genomic_DNA.
DR EMBL; GU732836; ADG60243.1; -; Genomic_DNA.
DR EMBL; LT985226; SPD96359.1; -; Genomic_DNA.
DR RefSeq; WP_012477587.1; NZ_UDID01000061.1.
DR AlphaFoldDB; Q5YCX9; -.
DR SMR; Q5YCX9; -.
DR GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030655; P:beta-lactam antibiotic catabolic process; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR045155; Beta-lactam_cat.
DR InterPro; IPR000871; Beta-lactam_class-A.
DR InterPro; IPR023650; Beta-lactam_class-A_AS.
DR PANTHER; PTHR35333; BETA-LACTAMASE; 1.
DR PANTHER; PTHR35333:SF3; BETA-LACTAMASE2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF13354; Beta-lactamase2; 1.
DR PRINTS; PR00118; BLACTAMASEA.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR PROSITE; PS00146; BETA_LACTAMASE_A; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance {ECO:0000256|ARBA:ARBA00023251,
KW ECO:0000256|RuleBase:RU361140};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361140};
KW Plasmid {ECO:0000313|EMBL:AAT97112.1}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..286
FT /note="Beta-lactamase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5015098088"
FT DOMAIN 45..258
FT /note="Beta-lactamase class A catalytic"
FT /evidence="ECO:0000259|Pfam:PF13354"
SQ SEQUENCE 286 AA; 31254 MW; 738F4266651F551A CRC64;
MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG RTLTAWRADE
RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA
AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA
SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGASERGARG
IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR
//
