ID Q5ZPH1_9TRYP Unreviewed; 304 AA.
AC Q5ZPH1;
DT 23-NOV-2004, integrated into UniProtKB/TrEMBL.
DT 23-NOV-2004, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE SubName: Full=Glycosomal glyceraldehyde phosphate dehydrogenase {ECO:0000313|EMBL:CAF04217.1};
DE Flags: Fragment;
GN Name=gGAPDH {ECO:0000313|EMBL:CAF04217.1};
OS Trypanosoma mega.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=71805 {ECO:0000313|EMBL:CAF04217.1};
RN [1] {ECO:0000313|EMBL:CAF04217.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC30038 {ECO:0000313|EMBL:CAF04217.1};
RX PubMed=15542100; DOI=10.1016/j.ijpara.2004.08.011;
RA Hamilton P.B., Stevens J.R., Gaunt M.W., Gidley J., Gibson W.C.;
RT "Trypanosomes are monophyletic: evidence from genes for glyceraldehyde
RT phosphate dehydrogenase and small subunit ribosomal RNA.";
RL Int. J. Parasitol. 34:1393-1404(2004).
CC -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase
CC family. {ECO:0000256|ARBA:ARBA00007406, ECO:0000256|RuleBase:RU000397}.
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DR EMBL; AJ620253; CAF04217.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5ZPH1; -.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016620; F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR020831; GlycerAld/Erythrose_P_DH.
DR InterPro; IPR020830; GlycerAld_3-P_DH_AS.
DR InterPro; IPR020829; GlycerAld_3-P_DH_cat.
DR InterPro; IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR10836; GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE; 1.
DR PANTHER; PTHR10836:SF76; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-RELATED; 1.
DR Pfam; PF02800; Gp_dh_C; 1.
DR Pfam; PF00044; Gp_dh_N; 1.
DR PIRSF; PIRSF000149; GAP_DH; 1.
DR PRINTS; PR00078; G3PDHDRGNASE.
DR SMART; SM00846; Gp_dh_N; 1.
DR SUPFAM; SSF55347; Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00071; GAPDH; 1.
PE 3: Inferred from homology;
KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|PIRSR:PIRSR000149-3};
KW Nucleotide-binding {ECO:0000256|PIRSR:PIRSR000149-3};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 6..145
FT /note="Glyceraldehyde 3-phosphate dehydrogenase NAD(P)
FT binding"
FT /evidence="ECO:0000259|SMART:SM00846"
FT ACT_SITE 145
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR000149-1"
FT BINDING 17
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000149-3"
FT BINDING 71
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000149-3"
FT BINDING 113
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000149-3"
FT BINDING 144..146
FT /ligand="D-glyceraldehyde 3-phosphate"
FT /ligand_id="ChEBI:CHEBI:59776"
FT /evidence="ECO:0000256|PIRSR:PIRSR000149-2"
FT BINDING 176
FT /ligand="D-glyceraldehyde 3-phosphate"
FT /ligand_id="ChEBI:CHEBI:59776"
FT /evidence="ECO:0000256|PIRSR:PIRSR000149-2"
FT BINDING 205..206
FT /ligand="D-glyceraldehyde 3-phosphate"
FT /ligand_id="ChEBI:CHEBI:59776"
FT /evidence="ECO:0000256|PIRSR:PIRSR000149-2"
FT BINDING 228
FT /ligand="D-glyceraldehyde 3-phosphate"
FT /ligand_id="ChEBI:CHEBI:59776"
FT /evidence="ECO:0000256|PIRSR:PIRSR000149-2"
FT SITE 173
FT /note="Activates thiol group during catalysis"
FT /evidence="ECO:0000256|PIRSR:PIRSR000149-4"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:CAF04217.1"
FT NON_TER 304
FT /evidence="ECO:0000313|EMBL:CAF04217.1"
SQ SEQUENCE 304 AA; 32591 MW; 4299BEEBFA33BDD0 CRC64;
RIHGLLGTEI DVVAVVDMNT DAEYFAYQMK YDTVHGKFKY TVSTTKTDAS AAKDDTLVVN
GHRILCVKAQ RNPADLPWGK LGVEYVIEST GLFTLKSAAE GHLKGGARKV VISAPASGGA
KTLVMGVNHN EYNPSEHHVV SNASCTTNCL APIVHVMVKE GFGVQTGLMT TIHSYTATQK
TVDGVSVKDW RGGRAAAVNI IPSTTGAAKA VGMVIPSTQG KLTGMSFRVP TPDVSVVDLT
FTTTRDTSIK EIDAALKRAS ATYMKGILGY TDEELVSTDF INDNRSSIYD SKATLQNKGE
FQHT
//
