ID   Q60D84_BRAOL            Unreviewed;       505 AA.
AC   Q60D84;
DT   23-NOV-2004, integrated into UniProtKB/TrEMBL.
DT   23-NOV-2004, sequence version 1.
DT   27-MAR-2024, entry version 54.
DE   SubName: Full=2-isopropylmalate synthase {ECO:0000313|EMBL:AAV25874.1};
GN   Name=B19N3.4 {ECO:0000313|EMBL:AAV25874.1};
OS   Brassica oleracea (Wild cabbage).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3712 {ECO:0000313|EMBL:AAV25874.1};
RN   [1] {ECO:0000313|EMBL:AAV25874.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Gao M., Quiros C.F., McCombie W.R., Nascimento L., Balija V., Bell M.,
RA   de la Bastide M., Spiegel L., Zutavern T., Muller S., Miller B.,
RA   Katzenberger F., Andrade M.V., Dike S., O'Shaughnessy A., Palmer L.;
RT   "Broccoli genomic sequence.";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:AAV25874.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Gao M., Carlos Q.;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC       family. {ECO:0000256|RuleBase:RU003523}.
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DR   EMBL; AC149635; AAV25874.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q60D84; -.
DR   SMR; Q60D84; -.
DR   OMA; DTVGVMW; -.
DR   ExpressionAtlas; Q60D84; baseline.
DR   GO; GO:0046912; F:acyltransferase activity, acyl groups converted into alkyl on transfer; IEA:InterPro.
DR   GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR   GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR   GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR   CDD; cd07940; DRE_TIM_IPMS; 1.
DR   Gene3D; 1.10.238.260; -; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR000891; PYR_CT.
DR   PANTHER; PTHR10277; HOMOCITRATE SYNTHASE-RELATED; 1.
DR   PANTHER; PTHR10277:SF73; PYRUVATE CARBOXYLTRANSFERASE DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF00682; HMGL-like; 1.
DR   SUPFAM; SSF51569; Aldolase; 1.
DR   PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR   PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   3: Inferred from homology;
KW   Transferase {ECO:0000256|RuleBase:RU003523}.
FT   DOMAIN          81..355
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000259|PROSITE:PS50991"
FT   REGION          483..505
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   505 AA;  55599 MW;  6D9399E77793064F CRC64;
     MASSLLTSSG IIPTTSSNMV VRSFLPFGSV RLTRPYNKSS LLISCCSSVS KKAETSGTDL
     KTVVERWPEY IPNKLPDKNY VRVFDTTLRD GEQSPGAALT PPQKIEIARQ LAKLRVDIME
     VGFPVSSEEE FETIKTIAKT VGNEVDEETG YIPVICVIAR SKERDIKAAW ESVKYAKRPR
     IVIFTSTSDI HLKYKLKMTR EEVVEMVASS IRFAKSLGFE DIEFGCEDGG RSDKDYICKV
     FEEAIKAGAT TLACPDTVGI NMPHEYGKLV RYIKANTPGI DDVIFSAHCH NDLGVATANT
     IAGICAGARQ VEVTINGIGE RSGNAPLEEV VMALKCRGAF VMGGVYTRID TRQIMATSKM
     VQEYTGLYVQ PHKPIVGANC FVHESGIHQD GILKNRSTYE ILSPEDVGVV KSQNSGIVLG
     KLSGRHAVKG RLKELGYEIS DEKLNEVFSR FRDLTKQKKR VTDDDLRALV TCGDEIFSSE
     KLNGTNDNEI NRNGYVPSPQ ISSVV
//