ID UVRD_RICTY Reviewed; 658 AA.
AC Q68WT1;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 27-MAR-2024, entry version 96.
DE RecName: Full=DNA helicase II;
DE EC=5.6.2.4;
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000305};
GN Name=uvrD; OrderedLocusNames=RT0434;
OS Rickettsia typhi (strain ATCC VR-144 / Wilmington).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX NCBI_TaxID=257363;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC VR-144 / Wilmington;
RX PubMed=15317790; DOI=10.1128/jb.186.17.5842-5855.2004;
RA McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E.,
RA McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E.,
RA Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A., Hong C.,
RA Yu X.-J., Walker D.H., Weinstock G.M.;
RT "Complete genome sequence of Rickettsia typhi and comparison with sequences
RT of other Rickettsiae.";
RL J. Bacteriol. 186:5842-5855(2004).
CC -!- FUNCTION: Has both ATPase and helicase activities. Unwinds DNA duplexes
CC with 3' to 5' polarity with respect to the bound strand and initiates
CC unwinding most effectively when a single-stranded region is present.
CC Involved in the post-incision events of nucleotide excision repair and
CC methyl-directed mismatch repair (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000305}.
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DR EMBL; AE017197; AAU03911.1; -; Genomic_DNA.
DR AlphaFoldDB; Q68WT1; -.
DR SMR; Q68WT1; -.
DR KEGG; rty:RT0434; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_2_5; -.
DR Proteomes; UP000000604; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR01073; pcrA; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA replication; DNA-binding;
KW Helicase; Hydrolase; Isomerase; Nucleotide-binding.
FT CHAIN 1..658
FT /note="DNA helicase II"
FT /id="PRO_0000286461"
FT DOMAIN 12..293
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT DOMAIN 294..564
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00617"
FT BINDING 36..41
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT BINDING 291
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 658 AA; 75502 MW; 165BD9DA57F15914 CRC64;
MKDKMQNQNF MHTLNSEQKK AALHTEGPLL LLAGAGTGKT KVLTSRIANI IQQNLALPHN
ILAVTFTNKA AKEMSERVHN LINCYGVNIG TFHSMAAKIL RDQIENLNLG LNNKFTIISH
DDQLTLVKDI VKLKKDIDTK KYTPKLIHII ISRWKDQGLL PSKLSASDTN LPLQRVAKLI
YEEYQKNLLI SNVLDFGDLL LYNNELFIKN PEILKYYQEK YRYILIDEYQ DTNIAQYLWA
RMLASLYRNI CCVGDDDQSI YGWRGAEVGN ILRFEKDFAG ATIIKLEQNY RSTLPILAAA
SNVINNNKNR HSKTLWTDSA SGEKIKIISC LSDKEEARYI ACEIDKLVRE ERYHAGNIAI
LVRAGFQTRS FEEAFINSAM PYKIIGGLRF YERMEIRDVL AYIRISLNQN DNLALERIIN
VPKRAIGAAS LNKIRSYALE RNISNFVAIK EILETGIFKA KSYESLKDLL TKIDNWYEKF
ITDTPINVVK TILDDSGYLS MLQEEKTEEA FGRIENINEM LRAIAEFNDI HDFIEHSSLV
MENEVLETNY GGSVTIMTLH AAKGLEFDVV FLPGWEEGVF PSQRSLDEDG EKGLEEERRI
AYVGITRAKK DLYITHAESR KIFYEIVHSC PSRFINEIPD EITIRTSSMK KYNSFYKF
//
