ID Q68YT8_9FUSO Unreviewed; 522 AA.
AC Q68YT8;
DT 13-SEP-2004, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2004, sequence version 1.
DT 27-MAR-2024, entry version 87.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
DE Flags: Fragment;
GN Name=gyrB {ECO:0000313|EMBL:AAT76295.1};
OS Fusobacterium canifelinum.
OC Bacteria; Fusobacteriota; Fusobacteriia; Fusobacteriales; Fusobacteriaceae;
OC Fusobacterium.
OX NCBI_TaxID=285729 {ECO:0000313|EMBL:AAT76295.1};
RN [1] {ECO:0000313|EMBL:AAT76295.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC BAA-690 {ECO:0000313|EMBL:AAT76295.1};
RX PubMed=15616329; DOI=10.1128/AAC.49.1.434-437.2005;
RA Conrads G., Citron D.M., Goldstein E.J.;
RT "Genetic determinant of intrinsic quinolone resistance in Fusobacterium
RT canifelinum.";
RL Antimicrob. Agents Chemother. 49:434-437(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
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DR EMBL; AY660895; AAT76295.1; -; Genomic_DNA.
DR AlphaFoldDB; Q68YT8; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd16928; HATPase_GyrB-like; 1.
DR CDD; cd00822; TopoII_Trans_DNA_gyrase; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR InterPro; IPR018522; TopoIIA_CS.
DR InterPro; IPR006171; TOPRIM_domain.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR PRINTS; PR00418; TPI2FAMILY.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS00177; TOPOISOMERASE_II; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 422..522
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT NON_TER 522
FT /evidence="ECO:0000313|EMBL:AAT76295.1"
SQ SEQUENCE 522 AA; 57897 MW; D04BA9DD43F68FCE CRC64;
MEEIMSYEAQ NITVLEGLEA VRKRPGMYIG TTSERGLHHL VWEIVDNSVD EALAGYCNKI
EVKILPENII EVVDNGRGIP TDIHPKYGKS ALEIVLTVLH AGGKFENDNY KVSGGLHGVG
VSVVNALSEW LEVEVRKNGV VYYQKYHRGK PEEDVKIIGS CDEGEHGTIV RFKADGDIFE
TLIYNYFTLS NRLKELAYLN KGLTIVLSDL RKEEKKEEIY KFDGGILDFL NEIVKEEATI
IEKPFYVSSE QDNVGVDVTF TYTTSQNEVI YSFVNNINTH EGGTHVQGFR TALTKVINDV
GKAQGLLKDK DGKLMGNDIR EGVVGIVSTK IPQPQFEGQT KGKLGNSEVS GIVNTIVSNS
LKIFLEDNPN ITKIIIEKIL NSKKAREAAQ KARELVLRKS VLEVGSLPGK LADCTSKKAE
ECEIFIVEGD SAGGSAKQGR DRYNQAILPL RGKIINVEKA GLHKSLESSE IRAMVTAFGT
SIGETFDISK LRYGKIILMT DADVDGAHIR TLILTFLYRY MI
//
