ID Q6E9P5_LISMN Unreviewed; 510 AA.
AC Q6E9P5;
DT 16-AUG-2004, integrated into UniProtKB/TrEMBL.
DT 16-AUG-2004, sequence version 1.
DT 24-JAN-2024, entry version 113.
DE RecName: Full=Neutral metalloproteinase {ECO:0000256|RuleBase:RU366073};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU366073};
GN Name=mpl {ECO:0000313|EMBL:AAS85065.1};
GN ORFNames=GHO09_14145 {ECO:0000313|EMBL:HAA8491649.1};
OS Listeria monocytogenes.
OC Bacteria; Bacillota; Bacilli; Bacillales; Listeriaceae; Listeria.
OX NCBI_TaxID=1639 {ECO:0000313|EMBL:AAS85065.1};
RN [1] {ECO:0000313|EMBL:AAS85065.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NRRL 33031 {ECO:0000313|EMBL:AAS84855.1}, and NRRL 33106
RC {ECO:0000313|EMBL:AAS85065.1};
RX PubMed=15262937; DOI=10.1128/JB.186.15.4994-5002.2004;
RA Ward T.J., Gorski L., Borucki M.K., Mandrell R.E., Hutchins J., Pupedis K.;
RT "Intraspecific phylogeny and lineage group identification based on the prfA
RT virulence gene cluster of Listeria monocytogenes.";
RL J. Bacteriol. 186:4994-5002(2004).
RN [2] {ECO:0000313|EMBL:ABG57035.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Lm19927 {ECO:0000313|EMBL:ABG57035.1};
RA Papageorgiou N., Tselentis Y., Scoulica E.;
RT "Nucleotide sequence diversity of prfA gene cluster of Listeria
RT monocytogenes strains of clinical and food origin.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:ABG57035.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Lm19927 {ECO:0000313|EMBL:ABG57035.1};
RA Papageorgiou N., Tselentis Y., Scoulica E.;
RT "Nucleotide sequence of actA genes from clinical and environmental Listeria
RT monocytogenes isolates.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:ABY71503.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=FSL S4-887 {ECO:0000313|EMBL:ABY71503.1};
RX PubMed=18499533; DOI=10.1016/j.meegid.2008.04.006;
RA Orsi R.H., Maron S.B., Nightingale K.K., Jerome M., Tabor H., Wiedmann M.;
RT "Lineage specific recombination and positive selection in coding and
RT intragenic regions contributed to evolution of the main Listeria
RT monocytogenes virulence gene cluster.";
RL Infect. Genet. Evol. 8:566-576(2008).
RN [5] {ECO:0000313|EMBL:HAA8491649.1, ECO:0000313|Proteomes:UP000840567}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sam_F526FDD3-C0F7-43DB-B204-E231FEF9C926
RC {ECO:0000313|EMBL:HAA8491649.1};
RX PubMed=30286803;
RA Souvorov A., Agarwala R., Lipman D.J.;
RT "SKESA: strategic k-mer extension for scrupulous assemblies.";
RL Genome Biol. 19:153-153(2018).
RN [6] {ECO:0000313|EMBL:HAA8491649.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Sam_F526FDD3-C0F7-43DB-B204-E231FEF9C926
RC {ECO:0000313|EMBL:HAA8491649.1};
RG NCBI Pathogen Detection Project;
RL Submitted (OCT-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Extracellular zinc metalloprotease.
CC {ECO:0000256|RuleBase:RU366073}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947,
CC ECO:0000256|RuleBase:RU366073};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU366073}.
CC -!- SIMILARITY: Belongs to the peptidase M4 family.
CC {ECO:0000256|ARBA:ARBA00009388, ECO:0000256|RuleBase:RU366073}.
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DR EMBL; AY512409; AAS84855.1; -; Genomic_DNA.
DR EMBL; AY512444; AAS85065.1; -; Genomic_DNA.
DR EMBL; DQ309886; ABG57035.1; -; Genomic_DNA.
DR EMBL; EU372055; ABY71503.1; -; Genomic_DNA.
DR EMBL; DAAEQL010000011; HAA8491649.1; -; Genomic_DNA.
DR RefSeq; WP_012952055.1; NZ_WUEC01000009.1.
DR MEROPS; M04.008; -.
DR KEGG; lmom:IJ09_09350; -.
DR PATRIC; fig|1639.1010.peg.2672; -.
DR Proteomes; UP000840567; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09597; M4_TLP; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR025711; PepSY.
DR InterPro; IPR023612; Peptidase_M4.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR001570; Peptidase_M4_C_domain.
DR InterPro; IPR013856; Peptidase_M4_domain.
DR PANTHER; PTHR33794; BACILLOLYSIN; 1.
DR PANTHER; PTHR33794:SF1; BACILLOLYSIN; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF03413; PepSY; 1.
DR Pfam; PF01447; Peptidase_M4; 1.
DR Pfam; PF02868; Peptidase_M4_C; 1.
DR PRINTS; PR00730; THERMOLYSIN.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366073};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU366073};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU366073};
KW Secreted {ECO:0000256|RuleBase:RU366073};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU366073};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366073};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT CHAIN 25..510
FT /note="Neutral metalloproteinase"
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT /id="PRO_5015098247"
FT DOMAIN 60..109
FT /note="FTP"
FT /evidence="ECO:0000259|Pfam:PF07504"
FT DOMAIN 121..194
FT /note="PepSY"
FT /evidence="ECO:0000259|Pfam:PF03413"
FT DOMAIN 214..357
FT /note="Peptidase M4"
FT /evidence="ECO:0000259|Pfam:PF01447"
FT DOMAIN 360..509
FT /note="Peptidase M4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02868"
FT ACT_SITE 350
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
FT ACT_SITE 437
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
SQ SEQUENCE 510 AA; 57411 MW; FAC28FBB7AAEB270 CRC64;
MKSKLICIIM VIAFQAHFTM TVKADSVGEE KLQNNTQAKK TPADLKALPD SCEAKDFYKN
FKILDMTKDK LGVTHYTLAL SSGGYLTDND EIKVHVTPDN KITFINGDLQ QGQLRITNQI
KITEKNAIEK AFEAIGQSEA HVKSYVGNPV KEKEIILNSR TKRLVYNIKL IFAEPEVASW
IVQVDVETGA ILKKQNMLSE VERADTHKDF QALGKGANRL LQRPLHVMKI NDLFYLVDRT
HKGLIRTFDL KHNTDTSFGK VVSNKTNMFT DPEFSSAVDA HFYASEVYEY YKNVHQLESL
DGKGGEIDSF VHYGLNCNNA FWDGQEILYG DGDKKNFKPF SCAKTIVGHE LTHAVIQYSA
GLEYEGQSGA LNESFADVFG YFIAPNHWLI GEDVCVRGSR DGRIRSIKDP DKYNQAAHMK
DYESLPITEE GDWGGVHYNS GIPNKAAYNT ITKLGKEKTE QLYFRALKYY LTKKSQFTDA
KKALQQAAKD LYGEDASKKV AEAWEAVGVN
//
