ID Q6ER83_ORYSJ Unreviewed; 802 AA.
AC Q6ER83;
DT 16-AUG-2004, integrated into UniProtKB/TrEMBL.
DT 16-AUG-2004, sequence version 1.
DT 24-JAN-2024, entry version 127.
DE RecName: Full=digalactosyldiacylglycerol synthase {ECO:0000256|ARBA:ARBA00024055};
DE EC=2.4.1.241 {ECO:0000256|ARBA:ARBA00024055};
GN OrderedLocusNames=Os02g0539100 {ECO:0000313|EMBL:BAS79088.1};
GN ORFNames=OSNPB_020539100 {ECO:0000313|EMBL:BAS79088.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947 {ECO:0000313|EMBL:BAS79088.1, ECO:0000313|Proteomes:UP000059680};
RN [1] {ECO:0000313|EMBL:BAG99949.1}
RP NUCLEOTIDE SEQUENCE.
RA Kikuchi S., Satoh K., Nagata T., Kawagashira N., Doi K., Kishimoto N.,
RA Yazaki J., Ishikawa M., Yamada H., Ooka H., Hotta I., Kojima K., Namiki T.,
RA Ohneda E., Yahagi W., Suzuki K., Li C., Ohtsuki K., Shishiki T., Otomo Y.,
RA Murakami K., Iida Y., Sugano S., Fujimura T., Suzuki Y., Tsunoda Y.,
RA Kurosaki T., Kodama T., Masuda H., Kobayashi M., Xie Q., Lu M.,
RA Narikawa R., Sugiyama A., Mizuno K., Yokomizo S., Niikura J., Ikeda R.,
RA Ishibiki J., Kawamata M., Yoshimura A., Miura J., Kusumegi T., Oka M.,
RA Ryu R., Ueda M., Matsubara K., Kawai J., Carninci P., Adachi J., Aizawa K.,
RA Arakawa T., Fukuda S., Hara A., Hashidume W., Hayatsu N., Imotani K.,
RA Ishii Y., Itoh M., Kagawa I., Kondo S., Konno H., Miyazaki A., Osato N.,
RA Ota Y., Saito R., Sasaki D., Sato K., Shibata K., Shinagawa A., Shiraki T.,
RA Yoshino M., Hayashizaki Y.;
RT "Collection, Mapping, and Annotation of Over 28,000 cDNA Clones from
RT japonica Rice.";
RL Science 301:376-379(2003).
RN [2] {ECO:0000313|EMBL:BAG99949.1}
RP NUCLEOTIDE SEQUENCE.
RA Adachi J., Aizawa K., Akimura T., Arakawa T., Carninci P., Doi K.,
RA Fujimura T., Fukuda S., Hanagaki T., Hara A., Hashizume W., Hayashida K.,
RA Hayashizaki Y., Hayatsu N., Hiramoto K., Hiraoka T., Hori F., Hotta I.,
RA Iida J., Iida Y., Ikeda R., Imamura K., Imotani K., Ishibiki J., Ishii Y.,
RA Ishikawa M., Itoh M., Kagawa I., Kanagawa S., Katoh H., Kawagashira N.,
RA Kawai J., Kawamata M., Kikuchi S., Kishikawa-Hirozane T., Kishimoto N.,
RA Kobayashi M., Kodama T., Kojima K., Kojima Y., Kondo S., Konno H.,
RA Kouda M., Koya S., Kurihara C., Kurosaki T., Kusumegi T., Li C., Lu M.,
RA Masuda H., Matsubara K., Matsuyama T., Miura J., Miyazaki A., Mizuno K.,
RA Murakami K., Murata M., Nagata T., Nakahama Y., Nakamura M., Namiki T.,
RA Narikawa R., Niikura J., Nishi K., Nomura K., Numasaki R., Ohneda E.,
RA Ohno M., Ohtsuki K., Oka M., Ooka H., Osato N., Ota Y., Otomo Y., Ryu R.,
RA Saitoh H., Sakai C., Sakai K., Sakazume N., Sano H., Sasaki D., Sato K.,
RA Satoh K., Shibata K., Shinagawa A., Shiraki T., Shishiki T., Sogabe Y.,
RA Sugano S., Sugiyama A., Suzuki K., Suzuki Y., Tagami M., Tagami-Takeda Y.,
RA Tagawa A., Takahashi F., Takaku-Akahira S., Tanaka T., Tomaru A., Toya T.,
RA Tsunoda Y., Ueda M., Waki K., Xie Q., Yahagi W., Yamada H., Yamamoto M.,
RA Yasunishi A., Yazaki J., Yokomizo S., Yoshimura A.;
RT "Collection, mapping, and annotation of 28K full-length cDNA clones from
RT japonica rice.";
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Proteomes:UP000059680}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare {ECO:0000313|Proteomes:UP000059680};
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RA Matsumoto T., Wu J., Kanamori H., Katayose Y., Fujisawa M., Namiki N.,
RA Mizuno H., Yamamoto K., Antonio B.A., Baba T., Sakata K., Nagamura Y.,
RA Aoki H., Arikawa K., Arita K., Bito T., Chiden Y., Fujitsuka N.,
RA Fukunaka R., Hamada M., Harada C., Hayashi A., Hijishita S., Honda M.,
RA Hosokawa S., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M.,
RA Ito K., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K.,
RA Karasawa W., Kurita K., Katagiri S., Kikuta A., Kobayashi H., Kobayashi N.,
RA Machita K., Maehara T., Masukawa M., Mizubayashi T., Mukai Y., Nagasaki H.,
RA Nagata Y., Naito S., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M.,
RA Meguro A., Negishi M., Ohta I., Ohta T., Okamoto M., Ono N., Saji S.,
RA Sakaguchi M., Sakai K., Shibata M., Shimokawa T., Song J., Takazaki Y.,
RA Terasawa K., Tsugane M., Tsuji K., Ueda S., Waki K., Yamagata H.,
RA Yamamoto M., Yamamoto S., Yamane H., Yoshiki S., Yoshihara R., Yukawa K.,
RA Zhong H., Yano M., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Bera J., Fadrosh D., Jin S., Johri S., Kim M.,
RA Overton L., Reardon M., Tsitrin T., Vuong H., Weaver B., Ciecko A.,
RA Tallon L., Jackson J., Pai G., Aken S.V., Utterback T., Reidmuller S.,
RA Feldblyum T., Hsiao J., Zismann V., Iobst S., de Vazeille A.R., Buell C.R.,
RA Ying K., Li Y., Lu T., Huang Y., Zhao Q., Feng Q., Zhang L., Zhu J.,
RA Weng Q., Mu J., Lu Y., Fan D., Liu Y., Guan J., Zhang Y., Yu S., Liu X.,
RA Zhang Y., Hong G., Han B., Choisne N., Demange N., Orjeda G., Samain S.,
RA Cattolico L., Pelletier E., Couloux A., Segurens B., Wincker P., D'Hont A.,
RA Scarpelli C., Weissenbach J., Salanoubat M., Quetier F., Yu Y., Kim H.R.,
RA Rambo T., Currie J., Collura K., Luo M., Yang T., Ammiraju J.S.S.,
RA Engler F., Soderlund C., Wing R.A., Palmer L.E., de la Bastide M.,
RA Spiegel L., Nascimento L., Zutavern T., O'Shaughnessy A., Dike S.,
RA Dedhia N., Preston R., Balija V., McCombie W.R., Chow T., Chen H.,
RA Chung M., Chen C., Shaw J., Wu H., Hsiao K., Chao Y., Chu M., Cheng C.,
RA Hour A., Lee P., Lin S., Lin Y., Liou J., Liu S., Hsing Y., Raghuvanshi S.,
RA Mohanty A., Bharti A.K., Gaur A., Gupta V., Kumar D., Ravi V., Vij S.,
RA Kapur A., Khurana P., Khurana P., Khurana J.P., Tyagi A.K., Gaikwad K.,
RA Singh A., Dalal V., Srivastava S., Dixit A., Pal A.K., Ghazi I.A.,
RA Yadav M., Pandit A., Bhargava A., Sureshbabu K., Batra K., Sharma T.R.,
RA Mohapatra T., Singh N.K., Messing J., Nelson A.B., Fuks G., Kavchok S.,
RA Keizer G., Linton E., Llaca V., Song R., Tanyolac B., Young S., Ho-Il K.,
RA Hahn J.H., Sangsakoo G., Vanavichit A., de Mattos Luiz.A.T., Zimmer P.D.,
RA Malone G., Dellagostin O., de Oliveira A.C., Bevan M., Bancroft I.,
RA Minx P., Cordum H., Wilson R., Cheng Z., Jin W., Jiang J., Leong S.A.,
RA Iwama H., Gojobori T., Itoh T., Niimura Y., Fujii Y., Habara T., Sakai H.,
RA Sato Y., Wilson G., Kumar K., McCouch S., Juretic N., Hoen D., Wright S.,
RA Bruskiewich R., Bureau T., Miyao A., Hirochika H., Nishikawa T.,
RA Kadowaki K., Sugiura M., Burr B., Sasaki T.;
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4] {ECO:0000313|EMBL:BAS79088.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=23299411; DOI=10.1093/pcp/pcs183;
RA Sakai H., Lee S.S., Tanaka T., Numa H., Kim J., Kawahara Y., Wakimoto H.,
RA Yang C.C., Iwamoto M., Abe T., Yamada Y., Muto A., Inokuchi H., Ikemura T.,
RA Matsumoto T., Sasaki T., Itoh T.;
RT "Rice Annotation Project Database (RAP-DB): an integrative and interactive
RT database for rice genomics.";
RL Plant Cell Physiol. 54:E6-E6(2013).
RN [5] {ECO:0000313|EMBL:BAS79088.1, ECO:0000313|Proteomes:UP000059680}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare {ECO:0000313|Proteomes:UP000059680};
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6] {ECO:0000313|EMBL:BAS79088.1}
RP NUCLEOTIDE SEQUENCE.
RA Sakai H., Kawahara Y., Matsumoto T., Buell C.R., Itoh T.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + UDP-alpha-
CC D-galactose = 1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-
CC galactosyl]-sn-glycerol + H(+) + UDP; Xref=Rhea:RHEA:10520,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17615, ChEBI:CHEBI:28396,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.241;
CC Evidence={ECO:0000256|ARBA:ARBA00023914};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004370}.
CC Plastid, chloroplast outer membrane {ECO:0000256|ARBA:ARBA00024013}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC Glycosyltransferase 4 subfamily. {ECO:0000256|ARBA:ARBA00009481}.
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DR EMBL; AK120285; BAG99949.1; -; mRNA.
DR EMBL; AP014958; BAS79088.1; -; Genomic_DNA.
DR RefSeq; XP_015626791.1; XM_015771305.1.
DR AlphaFoldDB; Q6ER83; -.
DR STRING; 39947.Q6ER83; -.
DR CAZy; GT4; Glycosyltransferase Family 4.
DR PaxDb; 39947-Q6ER83; -.
DR EnsemblPlants; Os02t0539100-01; Os02t0539100-01; Os02g0539100.
DR GeneID; 4329584; -.
DR Gramene; Os02t0539100-01; Os02t0539100-01; Os02g0539100.
DR KEGG; osa:4329584; -.
DR eggNOG; ENOG502QQ73; Eukaryota.
DR HOGENOM; CLU_011647_0_1_1; -.
DR OMA; FWSEPAK; -.
DR OrthoDB; 276624at2759; -.
DR Proteomes; UP000059680; Chromosome 2.
DR GO; GO:0009707; C:chloroplast outer membrane; IBA:GO_Central.
DR GO; GO:0046481; F:digalactosyldiacylglycerol synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0035250; F:UDP-galactosyltransferase activity; IBA:GO_Central.
DR GO; GO:0019375; P:galactolipid biosynthetic process; IBA:GO_Central.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IEA:EnsemblPlants.
DR GO; GO:0009809; P:lignin biosynthetic process; IEA:EnsemblPlants.
DR GO; GO:0031408; P:oxylipin biosynthetic process; IEA:EnsemblPlants.
DR GO; GO:0009266; P:response to temperature stimulus; IEA:EnsemblPlants.
DR CDD; cd01635; Glycosyltransferase_GTB-type; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR InterPro; IPR044525; DGDG1/2.
DR InterPro; IPR001296; Glyco_trans_1.
DR PANTHER; PTHR46132:SF5; DIGALACTOSYLDIACYLGLYCEROL SYNTHASE; 1.
DR PANTHER; PTHR46132; DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 2: Evidence at transcript level;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Membrane {ECO:0000256|ARBA:ARBA00022805};
KW Plastid {ECO:0000256|ARBA:ARBA00022805};
KW Plastid outer membrane {ECO:0000256|ARBA:ARBA00022805};
KW Reference proteome {ECO:0000313|Proteomes:UP000059680};
KW Transferase {ECO:0000256|ARBA:ARBA00022676}.
FT DOMAIN 561..665
FT /note="Glycosyl transferase family 1"
FT /evidence="ECO:0000259|Pfam:PF00534"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 802 AA; 89310 MW; 05EB48ED2FEB1F70 CRC64;
MEARASPSPS PQRPASMGRG GGGGGGGGDA SAALSFIYKG WREVRDSASA DLRLMRARAD
SLRTLADREL EHLLVSASTT VAAPAPPVAA GAPIAEVEFV RNRIQPKISE LRRQYAASGD
WELGLGRRVL EGWVAPPPPR GATTARVDLS GITAIRNALV PEVAGGGGAS TAWWSGDEME
EEEEKEWEVV RMIRGGLKEL ERRSQSSGEI LGGIPGPSEL VEKFKSRLKS FNMEPLGSKE
VPPLDLTEIM ANLVRQSGPF LDQLGLRREL RDKLVETLYS RQNHSLSADS SLLGDDNSTD
ELDLRIASVL QSTGYHTDDG LWNEPSKYEV SDNKRHVAIV TTASLPWMTG TAVNPLFRAA
YLARNSKQDV TLVVPWLCKS DQELVYPNSM TFSSPEEQET YIKKWLEERL GFESNFKISF
YPGKFSKERR SIIPAGDTSQ FISSREADIA ILEEPEHLNW YHHGNRWTDK FNHVVGVVHT
NYLEYIKREK NGALQAFLVK HINNWVTRAY CDKVLRLSAA TQDLPKSIIC NVHGVNPKFL
KIGDKIMADR ENGQQSFSKG AYFLGKMVWA KGYRELLDLL DKRKSDLQGF KLDVYGSGED
SQEVQSTAKK LNLNLNFFKG RDHADDSLHG YKVFINPSIS DVLCTATAEA LAMGKFVICA
EHPSNEFFMS FPNCLTYRTP EEFVARVNEA MAREPQPLTP EQRYNLSWEA ATERFMEYSD
LDKVLSQPVT EGVHRSKTRR TIQSNLSDAM DGGLAFAHHC LTGSEVLRLA TGAIPGTRDY
DKQHCVDMGL LPPQVQHPVY GW
//
