ID Q6LCH3_SHISO Unreviewed; 263 AA.
AC Q6LCH3;
DT 05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2004, sequence version 1.
DT 24-JAN-2024, entry version 71.
DE RecName: Full=Aminoglycoside (3'') (9) adenylyltransferase {ECO:0000256|ARBA:ARBA00035252, ECO:0000256|PIRNR:PIRNR000819};
DE EC=2.7.7.47 {ECO:0000256|ARBA:ARBA00035126, ECO:0000256|PIRNR:PIRNR000819};
GN Name=aadA {ECO:0000313|EMBL:AAD00086.1};
GN Synonyms=aadA1 {ECO:0000313|EMBL:ABN41478.1};
GN ORFNames=CI715_18830 {ECO:0000313|EMBL:OYL18343.1};
OS Shigella sonnei.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=624 {ECO:0000313|EMBL:AAD00086.1};
RN [1] {ECO:0000313|EMBL:AAD00086.1}
RP NUCLEOTIDE SEQUENCE.
RA Park Y.S.;
RL Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ABN41478.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=17242143; DOI=10.1128/AAC.01109-06;
RA Dubois V., Parizano M.P., Arpin C., Coulange L., Bezian M.C., Quentin C.;
RT "High genetic stability of integrons in clinical isolates of Shigella spp.
RT of worldwide origin.";
RL Antimicrob. Agents Chemother. 51:1333-1340(2007).
RN [3] {ECO:0000313|EMBL:ACV44201.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=SS98-34 {ECO:0000313|EMBL:ACV44201.1};
RX PubMed=20947666; DOI=10.1099/jmm.0.022517-0;
RA Chang C.Y., Lu P.L., Lin C.C., Lee T.M., Tsai M.Y., Chang L.L.;
RT "Integron types, gene cassettes, antimicrobial resistance genes and
RT plasmids of Shigella sonnei isolates from outbreaks and sporadic cases in
RT Taiwan.";
RL J. Med. Microbiol. 60:197-204(2011).
RN [4] {ECO:0000313|EMBL:AIX48252.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=M-X2D {ECO:0000313|EMBL:AIX48252.1};
RA Liang J., Lin M.;
RT "Investigation in the Spread of Antibiotic-resistant Bacteria in the
RT Jiulong River.";
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:OYL18343.1, ECO:0000313|Proteomes:UP000214987}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ECPUTH05 {ECO:0000313|EMBL:OYL18343.1,
RC ECO:0000313|Proteomes:UP000214987};
RA Fouts D., Sutton G., Nguyen K., Thamlikitkul V.;
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate;
CC Xref=Rhea:RHEA:63228, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:146260, ChEBI:CHEBI:146261;
CC Evidence={ECO:0000256|ARBA:ARBA00001672,
CC ECO:0000256|PIRNR:PIRNR000819};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + streptomycin = 3''-O-adenylylstreptomycin + diphosphate;
CC Xref=Rhea:RHEA:20245, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58007, ChEBI:CHEBI:58605; EC=2.7.7.47;
CC Evidence={ECO:0000256|ARBA:ARBA00035070,
CC ECO:0000256|PIRNR:PIRNR000819};
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DR EMBL; U57972; AAD00086.1; -; Genomic_DNA.
DR EMBL; DQ975275; ABN41478.1; -; Genomic_DNA.
DR EMBL; FJ748514; ACV44201.1; -; Genomic_DNA.
DR EMBL; KM924017; AIX48252.1; -; Genomic_DNA.
DR EMBL; NQDP01000131; OYL18343.1; -; Genomic_DNA.
DR SMR; Q6LCH3; -.
DR Proteomes; UP000214987; Unassembled WGS sequence.
DR GO; GO:0070566; F:adenylyltransferase activity; IEA:InterPro.
DR GO; GO:0009012; F:aminoglycoside 3''-adenylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR CDD; cd05403; NT_KNTase_like; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR InterPro; IPR024172; AadA/Aad9.
DR InterPro; IPR025184; AadA_C.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR002934; Polymerase_NTP_transf_dom.
DR Pfam; PF13427; AadA_C; 1.
DR Pfam; PF01909; NTP_transf_2; 1.
DR PIRSF; PIRSF000819; Streptomycin_3-adenylyltransf; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
PE 4: Predicted;
KW Antibiotic resistance {ECO:0000256|ARBA:ARBA00023251,
KW ECO:0000256|PIRNR:PIRNR000819};
KW ATP-binding {ECO:0000256|PIRNR:PIRNR000819};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR000819};
KW Nucleotidyltransferase {ECO:0000256|PIRNR:PIRNR000819};
KW Transferase {ECO:0000256|PIRNR:PIRNR000819, ECO:0000313|EMBL:AAD00086.1}.
FT DOMAIN 28..76
FT /note="Polymerase nucleotidyl transferase"
FT /evidence="ECO:0000259|Pfam:PF01909"
FT DOMAIN 152..254
FT /note="Adenylyltransferase AadA C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13427"
SQ SEQUENCE 263 AA; 29332 MW; D2CEAC8B7D403FD2 CRC64;
MREAVIAEVS TQLSEVVGVI ERHLEPTLLA VHLYGSAVDG GLKPHSDIDL LVTVTVRLDE
TTRRALINDL LETSASPGES EILRAVEVTI VVHDDIIPWR YPAKRELQFG EWQRNDILAG
IFEPATIDID LAILLTKARE HSVALVGPAA EELFDPVPEQ DLFEALNETL TLWNSPPDWA
GDERNVVLTL SRIWYSAVTG KIAPKDVAAD WAMERLPAQY QPVILEARQA YLGQEEDRLA
SRADQLEEFV HYVKGEITKV VGK
//
