ID Q6NHA4_CORDI Unreviewed; 415 AA.
AC Q6NHA4;
DT 05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 104.
DE SubName: Full=Precorrin-6Y C5,15-methyltransferase {ECO:0000313|EMBL:CAE49765.1};
GN OrderedLocusNames=DIP1236 {ECO:0000313|EMBL:CAE49765.1};
OS Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype
OS gravis).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
OC Corynebacteriaceae; Corynebacterium.
OX NCBI_TaxID=257309 {ECO:0000313|EMBL:CAE49765.1, ECO:0000313|Proteomes:UP000002198};
RN [1] {ECO:0000313|EMBL:CAE49765.1, ECO:0000313|Proteomes:UP000002198}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700971 / NCTC 13129 / Biotype gravis
RC {ECO:0000313|Proteomes:UP000002198};
RX PubMed=14602910; DOI=10.1093/nar/gkg874;
RA Cerdeno-Tarraga A.M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.,
RA Bentley S.D., Besra G.S., Churcher C., James K.D., De Zoysa A.,
RA Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S.,
RA Jagels K., Moule S., Quail M.A., Rabbinowitsch E., Rutherford K.,
RA Thomson N.R., Unwin L., Whitehead S., Barrell B.G.Parkhill.J.;
RT "The complete genome sequence and analysis of Corynebacterium diphtheriae
RT NCTC13129.";
RL Nucleic Acids Res. 31:6516-6523(2003).
RN [2] {ECO:0007829|PDB:3HM2}
RP X-RAY CRYSTALLOGRAPHY (2.21 ANGSTROMS) OF 236-410 IN COMPLEX WITH CALCIUM
RP AND MAGNESIUM.
RA Kim Y., Bigelow L., Keigher L., Joachimiak A.;
RT "Crystal Structure of Putative Precorrin-6Y C5,15-Methyltransferase
RT Targeted Domain from Corynebacterium diphtheriae.";
RL Submitted (MAY-2009) to the PDB data bank.
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004953}.
CC -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00005879}.
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DR EMBL; BX248357; CAE49765.1; -; Genomic_DNA.
DR PDB; 3HM2; X-ray; 2.21 A; A/B/C/D/E/F/G/H=236-410.
DR PDBsum; 3HM2; -.
DR AlphaFoldDB; Q6NHA4; -.
DR SMR; Q6NHA4; -.
DR STRING; 257309.DIP1236; -.
DR DNASU; 2649791; -.
DR KEGG; cdi:DIP1236; -.
DR HOGENOM; CLU_031955_0_0_11; -.
DR UniPathway; UPA00148; -.
DR EvolutionaryTrace; Q6NHA4; -.
DR Proteomes; UP000002198; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008276; F:protein methyltransferase activity; IEA:InterPro.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd11644; Precorrin-6Y-MT; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR000878; 4pyrrol_Mease.
DR InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR InterPro; IPR012818; CbiE.
DR InterPro; IPR006365; Cbl_synth_CobL.
DR InterPro; IPR014008; Cbl_synth_MTase_CbiT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR02467; CbiE; 1.
DR NCBIfam; TIGR02469; CbiT; 1.
DR PANTHER; PTHR43182; COBALT-PRECORRIN-6B C(15)-METHYLTRANSFERASE (DECARBOXYLATING); 1.
DR PANTHER; PTHR43182:SF1; COBALT-PRECORRIN-7 C(5)-METHYLTRANSFERASE; 1.
DR Pfam; PF00590; TP_methylase; 1.
DR PIRSF; PIRSF036428; CobL; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF53790; Tetrapyrrole methylase; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:3HM2}; Calcium {ECO:0007829|PDB:3HM2};
KW Cobalamin biosynthesis {ECO:0000256|ARBA:ARBA00022573};
KW Metal-binding {ECO:0007829|PDB:3HM2};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000002198};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 16..200
FT /note="Tetrapyrrole methylase"
FT /evidence="ECO:0000259|Pfam:PF00590"
FT BINDING 291
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0007829|PDB:3HM2"
FT BINDING 292
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:3HM2"
FT BINDING 295
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0007829|PDB:3HM2"
FT BINDING 295
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:3HM2"
SQ SEQUENCE 415 AA; 44123 MW; AC77FBA3129BF9A9 CRC64;
MGRSKNDDGQ AHTGSITVVG IGAGGVEELG AQARTALRQA TIIIGSKRQL GLLPEDFQGD
RIAWPSPLVP AIPALFAEFI DKEVVVLGSG DPMFHGIGST LHRLLPNHSM TVIPQPSSAS
LACARLGWAL DTTSVYSLVT HPIAHLTLAI EQGDRFLVLG RNHESPHEIC EHLISLGQST
AEVTVLSDIG SVSETITSGA ATDYPSNDSA LNVIAIRPLA ATRSRVPGLP DSEYVTDGQL
TKQHVRALAI SALAPKPHET LWDIGGGSGS IAIEWLRSTP QTTAVCFEIS EERRERILSN
AINLGVSDRI AVQQGAPRAF DDVPDNPDVI FIGGGLTAPG VFAAAWKRLP VGGRLVANAV
TVESEQMLWA LRKQFGGTIS SFAISHEHTV GSFITMKPAL PVHQWTVVKA LTKEL
//
