ID GRP1_XENLA Reviewed; 791 AA.
AC Q6NTL4;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 01-MAY-2013, entry version 75.
DE RecName: Full=RAS guanyl-releasing protein 1;
GN Name=rasgrp1;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus;
OC Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Functions as a diacylglycerol (DAG)-regulated nucleotide
CC exchange factor specifically activating Ras through the exchange
CC of bound GDP for GTP (By similarity).
CC -!- ENZYME REGULATION: Regulated by F-actin polymerization and
CC probably by calcium (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity). Cell
CC membrane; Peripheral membrane protein (By similarity). Golgi
CC apparatus membrane; Peripheral membrane protein (By similarity).
CC Endoplasmic reticulum membrane; Peripheral membrane protein (By
CC similarity). Note=Found both in the cytosol and associated with
CC membranes (By similarity).
CC -!- DOMAIN: The phorbol-ester/DAG-type zinc finger is the principal
CC mediator of the targeting to membranes and is required for
CC functional activation through DAG-binding (By similarity).
CC -!- SIMILARITY: Belongs to the RASGRP family.
CC -!- SIMILARITY: Contains 2 EF-hand domains.
CC -!- SIMILARITY: Contains 1 N-terminal Ras-GEF domain.
CC -!- SIMILARITY: Contains 1 phorbol-ester/DAG-type zinc finger.
CC -!- SIMILARITY: Contains 1 Ras-GEF domain.
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DR EMBL; BC068947; AAH68947.1; -; mRNA.
DR RefSeq; NP_001084532.2; NM_001091063.1.
DR UniGene; Xl.46699; -.
DR HSSP; P28867; 1PTQ.
DR ProteinModelPortal; Q6NTL4; -.
DR GeneID; 414479; -.
DR KEGG; xla:414479; -.
DR CTD; 10125; -.
DR Xenbase; XB-GENE-489815; rasgrp1.
DR HOVERGEN; HBG007513; -.
DR KO; K04350; -.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR Gene3D; 1.10.238.10; -; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR020454; DAG/PE-bd.
DR InterPro; IPR011992; EF-hand-like_dom.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR008937; Ras_GEF.
DR InterPro; IPR023578; Ras_GEF_dom.
DR InterPro; IPR001895; RasGRF_CDC25.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00130; C1_1; 1.
DR Pfam; PF13405; EF_hand_4; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR PRINTS; PR00008; DAGPEDOMAIN.
DR SMART; SM00109; C1; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; Ras_GEF; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 3.
DR PROSITE; PS00720; RASGEF; FALSE_NEG.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
DR PROSITE; PS00479; ZF_DAG_PE_1; 1.
DR PROSITE; PS50081; ZF_DAG_PE_2; 1.
PE 2: Evidence at transcript level;
KW Calcium; Cell membrane; Coiled coil; Cytoplasm; Differentiation;
KW Endoplasmic reticulum; Golgi apparatus;
KW Guanine-nucleotide releasing factor; Membrane; Metal-binding; Repeat;
KW Zinc; Zinc-finger.
FT CHAIN 1 791 RAS guanyl-releasing protein 1.
FT /FTId=PRO_0000316981.
FT DOMAIN 49 172 N-terminal Ras-GEF.
FT DOMAIN 201 432 Ras-GEF.
FT DOMAIN 466 501 EF-hand 1.
FT DOMAIN 493 528 EF-hand 2.
FT CA_BIND 479 490 1 (Potential).
FT CA_BIND 506 517 2 (Potential).
FT ZN_FING 537 587 Phorbol-ester/DAG-type.
FT REGION 53 106 Ras exchanger motif region; required for
FT transforming activity (By similarity).
FT COILED 728 783 Potential.
SQ SEQUENCE 791 AA; 90172 MW; CA0A023D63B8469E CRC64;
MGTVGKKKDR PAHGCSTIPK LALELKQIIH STTHPKVPAV TPLRVMMPLG KLSKGASLDE
LIQMCIQAFD LDGNMGQNNE LLQIMLTMHG FLIPSTELLI KLRTLYQDAM QNRSFSFCLR
ICYFIRYWIT ELWVMFKMDA KLTQTMEEFQ ELVRSHGEEL HWRLIDTAQI NSRDWSRKLT
QRIQSNCSKK RKVSLLFDHL EPQELAEHLT YLEFKAFRRI SFSDYQNYIV NGCVKDNPTM
ERSIALCNGI SQWVQLMVLS RPTPQLRAEV LTKFIHVAQK LHQLQNFNTL MAVIGGLCHS
SISRLKDTSS HVSHDVTKVL NEMTELLSSC RNYDNYRRAY NECTNFKIPI LGVHLKDLIA
LHEAMPDFLE ESKINVPKLH SLYNHINELI QLQNIAPPLE ANMDLVHLLT LSLDLYYTED
EMYELSYARE PRNYRAPPVT PSKPPVVADW ASGVSPKPDP KTISKHVQRM VDSVFKNYDL
DQDGYISQEE FEKIAASFPF SFCVMDKDRE GLISRQEITA YFMRASSICS KLGLGFLHNF
QETTYLRPTF CDNCAGFLWG VIKQGYRCKD CGMNCHKQCK ELVVFECKKR SKCSMGENNT
LSDAGQLEVI PAGGKGLTND CLGADEGPYS YPNGDGDIHT EVSKDRTIML MGSSAQKISV
RLQPAVKHRA TQTENETQSL CLQVPSPPRS RTPDLTSHLP ISPMPSPCPS PVPTRKKAYA
KWENKDSIRK ARAELRGGKA GIQELEKEKV FLKEENTALK IQLKDAHRRV ETLRAELRKY
VLDSDTHQKG S
//
