ID Q6TFK2_9NOST Unreviewed; 210 AA.
AC Q6TFK2;
DT 05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2004, sequence version 1.
DT 13-SEP-2023, entry version 44.
DE RecName: Full=nitrogenase {ECO:0000256|ARBA:ARBA00012773};
DE EC=1.18.6.1 {ECO:0000256|ARBA:ARBA00012773};
DE AltName: Full=Nitrogenase component I {ECO:0000256|ARBA:ARBA00030899};
DE Flags: Fragment;
GN Name=vnfDG {ECO:0000313|EMBL:AAQ99145.1};
OS Anabaena azotica.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae; Anabaena.
OX NCBI_TaxID=197653 {ECO:0000313|EMBL:AAQ99145.1};
RN [1] {ECO:0000313|EMBL:AAQ99145.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=FACHB-118 {ECO:0000313|EMBL:AAQ99145.1};
RX PubMed=16924483; DOI=10.1007/s00203-006-0150-4;
RA Boison G., Steingen C., Stal L.J., Bothe H.;
RT "The rice field cyanobacteria Anabaena azotica and Anabaena sp. CH1 express
RT vanadium-dependent nitrogenase.";
RL Arch. Microbiol. 186:367-376(2006).
CC -!- FUNCTION: The key enzymatic reactions in nitrogen fixation are
CC catalyzed by the nitrogenase complex, which has 2 components: the iron
CC protein (component 2) and a component 1 which is either a molybdenum-
CC iron protein, a vanadium-iron, or an iron-iron protein.
CC {ECO:0000256|ARBA:ARBA00004064}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=1.18.6.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000805};
CC -!- COFACTOR:
CC Name=iron-sulfur cluster; Xref=ChEBI:CHEBI:30408;
CC Evidence={ECO:0000256|ARBA:ARBA00001915};
CC -!- SUBUNIT: Hexamer of two alpha, two beta, and two delta chains.
CC {ECO:0000256|ARBA:ARBA00011515}.
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DR EMBL; AY422692; AAQ99145.1; -; Genomic_DNA.
DR AlphaFoldDB; Q6TFK2; -.
DR BRENDA; 1.18.6.2; 15626.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0009399; P:nitrogen fixation; IEA:InterPro.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 1.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR010143; Nase_comp1_asu.
DR InterPro; IPR014279; Nase_V-Fe_dsu.
DR InterPro; IPR004349; V/Nase_d_su.
DR NCBIfam; TIGR02930; vnfG_nitrog; 1.
DR PANTHER; PTHR43457; NITROGENASE MOLYBDENUM-IRON PROTEIN ALPHA CHAIN; 1.
DR PANTHER; PTHR43457:SF1; NITROGENASE MOLYBDENUM-IRON PROTEIN ALPHA CHAIN; 1.
DR Pfam; PF03139; AnfG_VnfG; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 8..75
FT /note="Nitrogenase/oxidoreductase component 1"
FT /evidence="ECO:0000259|Pfam:PF00148"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AAQ99145.1"
FT NON_TER 210
FT /evidence="ECO:0000313|EMBL:AAQ99145.1"
SQ SEQUENCE 210 AA; 23729 MW; C182A1486841A882 CRC64;
ARGQEGTIYI DDGNELEFFE VLEMIRPDVV LTGPRVGALV KKLHLPYVNG HGYHNGPYMG
FEGAVNMARD LYNAIYSPLM QLAAFDVRDD APKAPAKTKE IEHLNEKVTN ITTYIQERCL
WQFHSRAWDR EENINGVIKK AAELLRGERS VQETLTGKLH YADAKILVSE LKRNLPWIKE
LDKERVKSVL ESVKQNLVGI AISGSLNGEL
//
