ID Q6X2Y9_PSEAI Unreviewed; 469 AA.
AC Q6X2Y9;
DT 05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 101.
DE SubName: Full=Homospermidine synthase {ECO:0000313|EMBL:AAP94695.1, ECO:0000313|EMBL:ACD39124.1};
DE EC=2.5.1.44 {ECO:0000313|EMBL:CRP66677.1};
GN Name=CL-17 {ECO:0000313|EMBL:AAP94695.1};
GN Synonyms=hss {ECO:0000313|EMBL:CRP66677.1};
GN ORFNames=DY940_20665 {ECO:0000313|EMBL:RTS43730.1}, IPC1295_20660
GN {ECO:0000313|EMBL:RPM12304.1}, IPC737_00385
GN {ECO:0000313|EMBL:RPW78951.1}, PACL_0336
GN {ECO:0000313|EMBL:ACD39124.1}, PACL_0523
GN {ECO:0000313|EMBL:ACD39311.1}, PAERUG_P19_London_7_VIM_2_05_10_05189
GN {ECO:0000313|EMBL:CRP66677.1};
OS Pseudomonas aeruginosa.
OG Plasmid Rms148 {ECO:0000313|EMBL:BAG12112.1}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=287 {ECO:0000313|EMBL:AAP94695.1};
RN [1] {ECO:0000313|EMBL:AAP94695.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C {ECO:0000313|EMBL:AAP94695.1};
RX PubMed=14702321; DOI=10.1128/JB.186.2.518-534.2004;
RA Klockgether J., Reva O., Larbig K., Tummler B.;
RT "Sequence analysis of the mobile genome island pKLC102 of Pseudomonas
RT aeruginosa C.";
RL J. Bacteriol. 186:518-534(2004).
RN [2] {ECO:0000313|EMBL:ACD39124.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=PACS171b {ECO:0000313|EMBL:ACD39124.1}, and PACS88
RC {ECO:0000313|EMBL:ACD39311.1};
RX PubMed=18445516; DOI=10.1016/j.ygeno.2008.02.005;
RA Hayden H.S., Gillett W., Saenphimmachak C., Lim R., Zhou Y., Jacobs M.A.,
RA Chang J., Rohmer L., D'Argenio D.A., Palmieri A., Levy R., Haugen E.,
RA Wong G.K., Brittnacher M.J., Burns J.L., Miller S.I., Olson M.V., Kaul R.;
RT "Large-insert genome analysis technology detects structural variation in
RT Pseudomonas aeruginosa clinical strains from cystic fibrosis patients.";
RL Genomics 91:530-537(2008).
RN [3] {ECO:0000313|EMBL:BAG12112.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=Rms148 {ECO:0000313|EMBL:BAG12112.1};
RX PubMed=22878084; DOI=10.1016/j.plasmid.2012.07.004;
RA Yano H., Genka H., Ohtsubo Y., Nagata Y., Top E.M., Tsuda M.;
RT "Cointegrate-resolution of toluene-catabolic transposon Tn4651:
RT determination of crossover site and the segment required for full
RT resolution activity.";
RL Plasmid 69:24-35(2013).
RN [4] {ECO:0000313|EMBL:CRP66677.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=P19_London_7_VIM_2_05_10 {ECO:0000313|EMBL:CRP66677.1};
RA Radhakrishnan R., Underwood A., Al-Shahib A.;
RL Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|Proteomes:UP000045039}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P19_London_7_VIM_2_05_10 {ECO:0000313|Proteomes:UP000045039};
RA Radhakrishnan Rajesh, Underwood Anthony, Al-Shahib Ali;
RL Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|Proteomes:UP000284767, ECO:0000313|Proteomes:UP000284878}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CPHL10662 {ECO:0000313|EMBL:RPW78951.1,
RC ECO:0000313|Proteomes:UP000284878}, and PA-W36
RC {ECO:0000313|EMBL:RPM12304.1, ECO:0000313|Proteomes:UP000284767};
RA Feschi L., Jeukens J., Emond-Rheault J.-G., Kukavica-Ibrulj I., Boyle B.,
RA Levesque R.C.;
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|EMBL:RTS43730.1, ECO:0000313|Proteomes:UP000276985}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MRSN6241 {ECO:0000313|EMBL:RTS43730.1,
RC ECO:0000313|Proteomes:UP000276985};
RA Snesrud E., Mcgann P.;
RT "Pseudomonas aeruginosa Diversity Panel.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
RN [8] {ECO:0000313|Proteomes:UP000284767, ECO:0000313|Proteomes:UP000284878}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CPHL10662 {ECO:0000313|EMBL:RPW78951.1,
RC ECO:0000313|Proteomes:UP000284878}, and PA-W36
RC {ECO:0000313|EMBL:RPM12304.1, ECO:0000313|Proteomes:UP000284767};
RA Freschi L., Vincent A.T., Jeukens J., Emond-Rheault J.-G.,
RA Kukavica-Ibrulj I., Dupont M.-J., Charette S.J., Boyle B., Levesque R.C.;
RT "The Pseudomonas aeruginosa pan-genome provides new insights on its
RT population structure, horizontal gene transfer and pathogenicity.";
RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
RN [9] {ECO:0007829|PDB:6Y87}
RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) IN COMPLEX WITH NAD(+).
RX PubMed=34605434; DOI=10.1107/s2059798321008937;
RA Helfrich F., Scheidig A.J.;
RT "Structural and catalytic characterization of Blastochloris viridis and
RT Pseudomonas aeruginosa homospermidine synthases supports the essential role
RT of cation-pi interaction.";
RL Acta Crystallogr. D Struct. Biol. 77:1317-1335(2021).
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY258138; AAP94695.1; -; Genomic_DNA.
DR EMBL; EU595748; ACD39124.1; -; Genomic_DNA.
DR EMBL; EU595754; ACD39311.1; -; Genomic_DNA.
DR EMBL; AB375440; BAG12112.1; -; Genomic_DNA.
DR EMBL; CVVU01000234; CRP66677.1; -; Genomic_DNA.
DR EMBL; NSNE01000012; RPM12304.1; -; Genomic_DNA.
DR EMBL; NSTV01000001; RPW78951.1; -; Genomic_DNA.
DR EMBL; RXTL01000025; RTS43730.1; -; Genomic_DNA.
DR RefSeq; WP_003111047.1; NZ_WUDE01000008.1.
DR PDB; 6Y87; X-ray; 2.15 A; A/B/C/D/E/F=1-469.
DR PATRIC; fig|287.2579.peg.1610; -.
DR Proteomes; UP000045039; Unassembled WGS sequence.
DR Proteomes; UP000276985; Unassembled WGS sequence.
DR Proteomes; UP000284767; Unassembled WGS sequence.
DR Proteomes; UP000284878; Unassembled WGS sequence.
DR GO; GO:0047296; F:homospermidine synthase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.30.360.30; homospermidine synthase like; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR023181; Homospermid_syn-like_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032095; Sacchrp_dh-like_C.
DR InterPro; IPR005097; Sacchrp_dh_NADP.
DR Pfam; PF16653; Sacchrp_dh_C; 1.
DR Pfam; PF03435; Sacchrp_dh_NADP; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:6Y87};
KW Nucleotide-binding {ECO:0007829|PDB:6Y87};
KW Plasmid {ECO:0000313|EMBL:BAG12112.1};
KW Transferase {ECO:0000313|EMBL:CRP66677.1}.
FT DOMAIN 11..152
FT /note="Saccharopine dehydrogenase NADP binding"
FT /evidence="ECO:0000259|Pfam:PF03435"
FT DOMAIN 156..437
FT /note="Saccharopine dehydrogenase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16653"
FT BINDING 18
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0007829|PDB:6Y87"
FT BINDING 19
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0007829|PDB:6Y87"
FT BINDING 65
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0007829|PDB:6Y87"
FT BINDING 89
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0007829|PDB:6Y87"
FT BINDING 110
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0007829|PDB:6Y87"
FT BINDING 157
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0007829|PDB:6Y87"
FT BINDING 158
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0007829|PDB:6Y87"
FT BINDING 159
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0007829|PDB:6Y87"
SQ SEQUENCE 469 AA; 51800 MW; 19EEAC80DF64AAED CRC64;
MDFSINPPQR IVFVGLGTIA QSFLPLLSKV HDLSTLEIYA IDPKTPPLIE YFANSFGLKF
INSAIDQINY RDILVPILGE GTVLINLSTD VSSLALIELC RSAGALYLDT CIEPWKGGYD
DPTIPLHKRT NYHLREQMLS LKKRLGSGVT ALVAHGANPG LVSHFVKRAL LDLAEEILGD
CKKPSNKEQW AILSQRLGVK VIHVAEYDSQ ISQKSRERGE FVNTWSVHGF ISESQQPAEL
GWGSHERSLP TDASMHTDGC GAAIYIEKPG ASVRVKTWTP FNGPSLGYLV THHEAISIAD
FLTLRTADET YRPTVHYAYR PSDEAILSVH EWFGNDCMTP EKTKVLRPGD ILSGSDYLGV
LLMGHEKSSY WYGSILSIEK AKELATLNTA TTLQVAAGVL SGYLWILSHP SAGIIEAEDM
DHEVALSYIS QYLGELKGVY SDWNPTKNNP GTFSAIDSDS PWLFSNFVL
//
