ID WOX8_ARATH Reviewed; 325 AA.
AC Q6X7J5; Q9FNM4;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 24-JAN-2024, entry version 131.
DE RecName: Full=WUSCHEL-related homeobox 8 {ECO:0000303|PubMed:14711878};
DE AltName: Full=Protein STIMPY-LIKE {ECO:0000303|PubMed:17706632};
GN Name=WOX8 {ECO:0000303|PubMed:14711878};
GN Synonyms=STPL {ECO:0000303|PubMed:17706632};
GN OrderedLocusNames=At5g45980 {ECO:0000312|Araport:AT5G45980};
GN ORFNames=K15I22.18 {ECO:0000312|EMBL:BAB08243.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=14711878; DOI=10.1242/dev.00963;
RA Haecker A., Gross-Hardt R., Geiges B., Sarkar A., Breuninger H.,
RA Herrmann M., Laux T.;
RT "Expression dynamics of WOX genes mark cell fate decisions during early
RT embryonic patterning in Arabidopsis thaliana.";
RL Development 131:657-668(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17706632; DOI=10.1016/j.ydbio.2007.07.019;
RA Wu X., Chory J., Weigel D.;
RT "Combinations of WOX activities regulate tissue proliferation during
RT Arabidopsis embryonic development.";
RL Dev. Biol. 309:306-316(2007).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=21802295; DOI=10.1016/j.cub.2011.06.049;
RA Jeong S., Palmer T.M., Lukowitz W.;
RT "The RWP-RK factor GROUNDED promotes embryonic polarity by facilitating
RT YODA MAP kinase signaling.";
RL Curr. Biol. 21:1268-1276(2011).
RN [6]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION BY WRKY2.
RX PubMed=21316593; DOI=10.1016/j.devcel.2011.01.009;
RA Ueda M., Zhang Z., Laux T.;
RT "Transcriptional activation of Arabidopsis axis patterning genes WOX8/9
RT links zygote polarity to embryo development.";
RL Dev. Cell 20:264-270(2011).
RN [7]
RP INDUCTION BY CLE8, AND TISSUE SPECIFICITY.
RX PubMed=22427333; DOI=10.1105/tpc.111.094839;
RA Fiume E., Fletcher J.C.;
RT "Regulation of Arabidopsis embryo and endosperm development by the
RT polypeptide signaling molecule CLE8.";
RL Plant Cell 24:1000-1012(2012).
RN [8]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=22827849; DOI=10.1111/j.1365-313x.2012.05113.x;
RA Lie C., Kelsom C., Wu X.;
RT "WOX2 and STIMPY-LIKE/WOX8 promote cotyledon boundary formation in
RT Arabidopsis.";
RL Plant J. 72:674-682(2012).
CC -!- FUNCTION: Probable transcription factor, which may be involved in
CC embryonic patterning (PubMed:14711878). May be required for basal
CC embryo development after fertilization (PubMed:14711878). Acts
CC partially redundantly with STIP in promoting embryonic cell division
CC and proliferation (PubMed:17706632). Promotes cotyledon boundary
CC formation by maintaining the symmetry in CUC genes expression domains
CC (PubMed:22827849). {ECO:0000269|PubMed:17706632,
CC ECO:0000269|PubMed:22827849, ECO:0000303|PubMed:14711878}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- TISSUE SPECIFICITY: Expressed only in the egg cell (PubMed:21316593).
CC Not detected in the pollen tube (PubMed:21316593). Expressed in the
CC zygote, the basal cell, and later the suspensor (PubMed:21802295).
CC Expressed in all suspensor cells, except the hypophysis, and in the
CC embryo surrounding region (ESR) endosperm cells (PubMed:22427333).
CC Strongly expressed in the suspensor cells, with a weak expression also
CC detected throughout the developing embryo (PubMed:22827849).
CC {ECO:0000269|PubMed:21316593, ECO:0000269|PubMed:21802295,
CC ECO:0000269|PubMed:22427333, ECO:0000269|PubMed:22827849}.
CC -!- DEVELOPMENTAL STAGE: Detected in the egg cell and the central cell of
CC the embryo sac (PubMed:14711878). After fertilization, it is expressed
CC in the zygote (PubMed:14711878). After the first division of the
CC zygote, it is detected exclusively in the basal daughter cell, while
CC WOX2 is expressed in the apical daughter cell (PubMed:14711878).
CC Through the 16-cell stage, it is expressed in all descendants of the
CC basal daughter, the developing suspensor and the hypophyseal cell
CC (PubMed:14711878). After the hypophysis had divided, expression stops
CC in descendants, but remains present in the extra embryonic suspensor
CC (PubMed:14711878). Moreover it is found in the cellularized endosperm
CC of the micropylar region during the globular and heart stages of
CC embryogenesis (PubMed:14711878). Not expressed later in embryogenesis
CC or in postembryonic stages (PubMed:14711878). Activated in the zygote
CC after fertilization (PubMed:21316593). {ECO:0000269|PubMed:14711878,
CC ECO:0000269|PubMed:21316593}.
CC -!- INDUCTION: Up-regulated in the zygote after fertilization by the
CC transcription factor WRKY2 (PubMed:21316593). Up-regulated by CLE8
CC (PubMed:22427333). {ECO:0000269|PubMed:21316593,
CC ECO:0000269|PubMed:22427333}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, due to the redundancy with
CC WOX9 (PubMed:17706632). Wox8 and wox9 double mutants are embryo lethal,
CC with embryos disrupted as early as the first cell division in the
CC embryo proper (PubMed:17706632). {ECO:0000269|PubMed:17706632}.
CC -!- SIMILARITY: Belongs to the WUS homeobox family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08243.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY251400; AAP37138.1; -; mRNA.
DR EMBL; AB006698; BAB08243.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95323.1; -; Genomic_DNA.
DR RefSeq; NP_199410.2; NM_123966.3.
DR AlphaFoldDB; Q6X7J5; -.
DR SMR; Q6X7J5; -.
DR STRING; 3702.Q6X7J5; -.
DR PaxDb; 3702-AT5G45980-1; -.
DR EnsemblPlants; AT5G45980.1; AT5G45980.1; AT5G45980.
DR GeneID; 834638; -.
DR Gramene; AT5G45980.1; AT5G45980.1; AT5G45980.
DR KEGG; ath:AT5G45980; -.
DR Araport; AT5G45980; -.
DR TAIR; AT5G45980; WOX8.
DR eggNOG; ENOG502QVSY; Eukaryota.
DR HOGENOM; CLU_030463_0_0_1; -.
DR InParanoid; Q6X7J5; -.
DR OMA; YMHYSNC; -.
DR OrthoDB; 405722at2759; -.
DR PhylomeDB; Q6X7J5; -.
DR PRO; PR:Q6X7J5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q6X7J5; baseline and differential.
DR Genevisible; Q6X7J5; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0090451; P:cotyledon boundary formation; IGI:TAIR.
DR GO; GO:0048825; P:cotyledon development; IMP:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0030010; P:establishment of cell polarity; IMP:TAIR.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IGI:TAIR.
DR GO; GO:0050793; P:regulation of developmental process; IEA:InterPro.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR044557; WOX8/9-like.
DR PANTHER; PTHR47288:SF3; WUSCHEL-RELATED HOMEOBOX 8; 1.
DR PANTHER; PTHR47288; WUSCHEL-RELATED HOMEOBOX 9; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..325
FT /note="WUSCHEL-related homeobox 8"
FT /id="PRO_0000049375"
FT DNA_BIND 51..115
FT /note="Homeobox; WUS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
SQ SEQUENCE 325 AA; 36312 MW; 370B5CBBAD1CB809 CRC64;
MSSSNKNWPS MFKSKPCNNN HHHQHEIDTP SYMHYSNCNL SSSFSSDRIP DPKPRWNPKP
EQIRILESIF NSGTINPPRE EIQRIRIRLQ EYGQIGDANV FYWFQNRKSR AKHKLRVHHK
SPKMSKKDKT VIPSTDADHC FGFVNQETGL YPVQNNELVV TEPAGFLFPV HNDPSAAQSA
FGFGDFVVPV VTEEGMAFST VNNGVNLETN ENFDKIPAIN LYGGDGNGGG NCFPPLTVPL
TINQSQEKRD VGLSGGEDVG DNVYPVRMTV FINEMPIEVV SGLFNVKAAF GNDAVLINSF
GQPILTDEFG VTYQPLQNGA IYYLI
//
