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Database: UniProt
Entry: Q73H01
LinkDB: Q73H01
Original site: Q73H01 
ID   DNLJ_WOLPM              Reviewed;         662 AA.
AC   Q73H01;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   01-OCT-2014, entry version 79.
DE   RecName: Full=DNA ligase {ECO:0000255|HAMAP-Rule:MF_01588};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01588};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] {ECO:0000255|HAMAP-Rule:MF_01588};
GN   Name=ligA {ECO:0000255|HAMAP-Rule:MF_01588};
GN   OrderedLocusNames=WD_0776;
OS   Wolbachia pipientis wMel.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Anaplasmataceae; Wolbachieae; Wolbachia.
OX   NCBI_TaxID=163164;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15024419; DOI=10.1371/journal.pbio.0020069;
RA   Wu M., Sun L.V., Vamathevan J.J., Riegler M., DeBoy R.T.,
RA   Brownlie J.C., McGraw E.A., Martin W., Esser C., Ahmadinejad N.,
RA   Wiegand C., Madupu R., Beanan M.J., Brinkac L.M., Daugherty S.C.,
RA   Durkin A.S., Kolonay J.F., Nelson W.C., Mohamoud Y., Lee P.,
RA   Berry K.J., Young M.B., Utterback T.R., Weidman J.F., Nierman W.C.,
RA   Paulsen I.T., Nelson K.E., Tettelin H., O'Neill S.L., Eisen J.A.;
RT   "Phylogenomics of the reproductive parasite Wolbachia pipientis wMel:
RT   a streamlined genome overrun by mobile genetic elements.";
RL   PLoS Biol. 2:327-341(2004).
CC   -!- FUNCTION: DNA ligase that catalyzes the formation of
CC       phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl
CC       groups in double-stranded DNA using NAD as a coenzyme and as the
CC       energy source for the reaction. It is essential for DNA
CC       replication and repair of damaged DNA. {ECO:0000255|HAMAP-
CC       Rule:MF_01588}.
CC   -!- CATALYTIC ACTIVITY: NAD(+) + (deoxyribonucleotide)(n) +
CC       (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-
CC       ribonucleotide + (deoxyribonucleotide)(n+m). {ECO:0000255|HAMAP-
CC       Rule:MF_01588}.
CC   -!- COFACTOR: Magnesium or manganese. {ECO:0000255|HAMAP-
CC       Rule:MF_01588}.
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01588}.
CC   -!- SIMILARITY: Contains 1 BRCT domain. {ECO:0000255|HAMAP-
CC       Rule:MF_01588}.
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DR   EMBL; AE017196; AAS14465.1; -; Genomic_DNA.
DR   RefSeq; NP_966531.1; NC_002978.6.
DR   RefSeq; WP_010962827.1; NC_002978.6.
DR   ProteinModelPortal; Q73H01; -.
DR   STRING; 163164.WD0776; -.
DR   EnsemblBacteria; AAS14465; AAS14465; WD_0776.
DR   GeneID; 2737976; -.
DR   KEGG; wol:WD0776; -.
DR   PATRIC; 24030145; VBIWolEnd21207_0774.
DR   eggNOG; COG0272; -.
DR   HOGENOM; HOG000218458; -.
DR   KO; K01972; -.
DR   OMA; DSKITAN; -.
DR   OrthoDB; EOG6TTVM9; -.
DR   BioCyc; WEND163164:GJ8W-761-MONOMER; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.10190; -; 1.
DR   HAMAP; MF_01588; DNA_ligase_A; 1.
DR   InterPro; IPR001357; BRCT_dom.
DR   InterPro; IPR018239; DNA_ligase_AS.
DR   InterPro; IPR001679; DNAligase.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   InterPro; IPR004149; Znf_DNAligase_C4.
DR   Pfam; PF00533; BRCT; 1.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   Pfam; PF03119; DNA_ligase_ZBD; 1.
DR   PIRSF; PIRSF001604; LigA; 1.
DR   SMART; SM00292; BRCT; 1.
DR   SMART; SM00278; HhH1; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF52113; SSF52113; 1.
DR   TIGRFAMs; TIGR00575; dnlj; 1.
DR   PROSITE; PS50172; BRCT; 1.
DR   PROSITE; PS01055; DNA_LIGASE_N1; 1.
DR   PROSITE; PS01056; DNA_LIGASE_N2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; DNA damage; DNA repair; DNA replication; Ligase;
KW   Magnesium; Manganese; Metal-binding; NAD; Reference proteome; Zinc.
FT   CHAIN         1    662       DNA ligase.
FT                                /FTId=PRO_0000313511.
FT   DOMAIN      580    662       BRCT. {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   NP_BIND      32     36       NAD. {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   NP_BIND      75     76       NAD. {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   ACT_SITE    108    108       N6-AMP-lysine intermediate.
FT                                {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   METAL       389    389       Zinc. {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   METAL       392    392       Zinc. {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   METAL       407    407       Zinc. {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   METAL       413    413       Zinc. {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   BINDING     106    106       NAD. {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   BINDING     129    129       NAD. {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   BINDING     164    164       NAD. {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   BINDING     271    271       NAD. {ECO:0000255|HAMAP-Rule:MF_01588}.
FT   BINDING     295    295       NAD. {ECO:0000255|HAMAP-Rule:MF_01588}.
SQ   SEQUENCE   662 AA;  74348 MW;  EFBFF7CEB82D9F5C CRC64;
     MTNLEKMREK LQDQINYHNV LYHQKNKPEI SDAEYDELKK KLAEIEPEIY ATQDSVGAPP
     DERFSKVEHQ EPMLSLENAY DEQGVEKFLS KIKRFLIEDK IEILCEPKID GLSFSAIYED
     GRFVKAATRG DGFVGEDVTH NVATIKGFPK FLQGVQGRLE VRGEIYISNS DFLKLNENDE
     FANPRNAAAG SLKQLDANIT ASRPLRYFAY SLIGGAEKSQ SEVLNKLEAL GFCVNEHQSL
     TSSLDGMLKF YNEVYNCRYN LDYDIDGIVY KVNDLVLQNR LGNTHKAPRS ALAYKFSAVY
     AKTKLNKIFI QVGRTGVLTP VADLVPVNVG GVLVSRASLH NQDEIKRKDI REGDIVTIKR
     AGDVIPQIVE VSRDSRLPNT PEFVFPEVCP ECGSKVRQVE GEAAVRCPEE FACKAQMIEK
     LKHFVSKDAF DIVGLGDKQI EFFYDLGLIK QIHDIFTLEE RLDEFNLEEQ SGWGEKSIAN
     LLNSIQSRRV ITLDRFIFSL GIRFIGQVAA ELLADYYVSY NNWYNSMIEL SLDNTELVGI
     DGIGKKGAES LESFFSKEHN IKMLNDLTAC LQILPVSSNS SNSVLNNKII VFTGKLLAMS
     RGEAKVRAKT LGAKVSSHLS AKTDYLIAGE KPGSKYKKAV ELGVEILDED QWHKVISLGV
     FK
//
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