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Database: UniProt
Entry: Q7M7H8
LinkDB: Q7M7H8
Original site: Q7M7H8 
ID   GSHB_PHOLL              Reviewed;         316 AA.
AC   Q7M7H8;
DT   15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   01-OCT-2014, entry version 69.
DE   RecName: Full=Glutathione synthetase {ECO:0000255|HAMAP-Rule:MF_00162};
DE            EC=6.3.2.3 {ECO:0000255|HAMAP-Rule:MF_00162};
DE   AltName: Full=GSH synthetase {ECO:0000255|HAMAP-Rule:MF_00162};
DE            Short=GSH-S {ECO:0000255|HAMAP-Rule:MF_00162};
DE            Short=GSHase {ECO:0000255|HAMAP-Rule:MF_00162};
DE   AltName: Full=Glutathione synthase {ECO:0000255|HAMAP-Rule:MF_00162};
GN   Name=gshB {ECO:0000255|HAMAP-Rule:MF_00162};
GN   OrderedLocusNames=plu1184;
OS   Photorhabdus luminescens subsp. laumondii (strain TT01).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Photorhabdus.
OX   NCBI_TaxID=243265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TT01;
RX   PubMed=14528314; DOI=10.1038/nbt886;
RA   Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA   Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA   Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S.,
RA   Medigue C., Lanois A., Powell K., Siguier P., Vincent R., Wingate V.,
RA   Zouine M., Glaser P., Boemare N., Danchin A., Kunst F.;
RT   "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT   luminescens.";
RL   Nat. Biotechnol. 21:1307-1313(2003).
CC   -!- CATALYTIC ACTIVITY: ATP + gamma-L-glutamyl-L-cysteine + glycine =
CC       ADP + phosphate + glutathione. {ECO:0000255|HAMAP-Rule:MF_00162}.
CC   -!- COFACTOR: Binds 1 magnesium or manganese ion per subunit.
CC       {ECO:0000250}.
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 2/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00162}.
CC   -!- SIMILARITY: Belongs to the prokaryotic GSH synthase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00162}.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain. {ECO:0000255|HAMAP-
CC       Rule:MF_00162}.
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DR   EMBL; BX571862; CAE13478.1; -; Genomic_DNA.
DR   RefSeq; NP_928496.1; NC_005126.1.
DR   ProteinModelPortal; Q7M7H8; -.
DR   SMR; Q7M7H8; 1-315.
DR   STRING; 243265.plu1184; -.
DR   EnsemblBacteria; CAE13478; CAE13478; plu1184.
DR   GeneID; 2801148; -.
DR   KEGG; plu:plu1184; -.
DR   PATRIC; 20506063; VBIPhoLum48522_1303.
DR   GenoList; plu1184; -.
DR   eggNOG; COG0189; -.
DR   HOGENOM; HOG000265022; -.
DR   KO; K01920; -.
DR   OMA; DWKIARA; -.
DR   OrthoDB; EOG6WMHWB; -.
DR   UniPathway; UPA00142; UER00210.
DR   GO; GO:0005829; C:cytosol; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004363; F:glutathione synthase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.30.470.20; -; 1.
DR   Gene3D; 3.40.50.20; -; 1.
DR   HAMAP; MF_00162; GSH_S; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR006284; Glut_synth_pro.
DR   InterPro; IPR004218; GSHS_ATP-bd.
DR   InterPro; IPR004215; GSHS_N.
DR   InterPro; IPR016185; PreATP-grasp_dom.
DR   Pfam; PF02955; GSH-S_ATP; 1.
DR   Pfam; PF02951; GSH-S_N; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR01380; glut_syn; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase;
KW   Magnesium; Manganese; Metal-binding; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN         1    316       Glutathione synthetase.
FT                                /FTId=PRO_0000197472.
FT   DOMAIN      125    311       ATP-grasp. {ECO:0000255|HAMAP-
FT                                Rule:MF_00162}.
FT   NP_BIND     151    207       ATP. {ECO:0000255|HAMAP-Rule:MF_00162}.
FT   METAL       281    281       Magnesium or manganese.
FT                                {ECO:0000255|HAMAP-Rule:MF_00162}.
FT   METAL       283    283       Magnesium or manganese.
FT                                {ECO:0000255|HAMAP-Rule:MF_00162}.
SQ   SEQUENCE   316 AA;  35765 MW;  4D13BB42272E38AF CRC64;
     MIKLGIVMDP ISSINIKKDT SFAMLLEAQR RSWELHYMEM SDLYLHQGEA RARTRLLQVE
     NHPQQWYQFD REQDLALETL DVILMRKDPP FDTEYIYATY ILERAEEKGT LIVNKPQSLR
     DCNEKLFTAW FPELTPDTLV TRNAAHLREF HQKHGDVIFK PLDGMGGASI FRLKKDDPNV
     GVIIETLTEH GNRFCMAQNF LPAIKEGDKR VLIVDGEPVP YCLARIPAQG ETRGNLAAGG
     RGEARPLSES DWAIARAVAP TLQEKGLIFV GLDIIGDKLT EINVTSPTCA REIEAAFPDI
     SITGMLMNAI EERLEK
//
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