GenomeNet

Database: UniProt
Entry: Q7N6K4
LinkDB: Q7N6K4
Original site: Q7N6K4 
ID   MAO1_PHOLL              Reviewed;         565 AA.
AC   Q7N6K4;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   26-NOV-2014, entry version 75.
DE   RecName: Full=NAD-dependent malic enzyme {ECO:0000255|HAMAP-Rule:MF_01619};
DE            Short=NAD-ME {ECO:0000255|HAMAP-Rule:MF_01619};
DE            EC=1.1.1.38 {ECO:0000255|HAMAP-Rule:MF_01619};
GN   Name=maeA {ECO:0000255|HAMAP-Rule:MF_01619};
GN   OrderedLocusNames=plu1546;
OS   Photorhabdus luminescens subsp. laumondii (strain TT01).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Photorhabdus.
OX   NCBI_TaxID=243265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TT01;
RX   PubMed=14528314; DOI=10.1038/nbt886;
RA   Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA   Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA   Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S.,
RA   Medigue C., Lanois A., Powell K., Siguier P., Vincent R., Wingate V.,
RA   Zouine M., Glaser P., Boemare N., Danchin A., Kunst F.;
RT   "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT   luminescens.";
RL   Nat. Biotechnol. 21:1307-1313(2003).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC       {ECO:0000255|HAMAP-Rule:MF_01619}.
CC   -!- CATALYTIC ACTIVITY: Oxaloacetate = pyruvate + CO(2).
CC       {ECO:0000255|HAMAP-Rule:MF_01619}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01619};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01619};
CC       Note=Divalent metal cations. Prefers magnesium or manganese.
CC       {ECO:0000255|HAMAP-Rule:MF_01619};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01619}.
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
CC       {ECO:0000255|HAMAP-Rule:MF_01619}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; BX571864; CAE13839.1; -; Genomic_DNA.
DR   RefSeq; NP_928837.1; NC_005126.1.
DR   ProteinModelPortal; Q7N6K4; -.
DR   STRING; 243265.plu1546; -.
DR   PRIDE; Q7N6K4; -.
DR   EnsemblBacteria; CAE13839; CAE13839; plu1546.
DR   GeneID; 2801541; -.
DR   KEGG; plu:plu1546; -.
DR   PATRIC; 20506967; VBIPhoLum48522_1726.
DR   GenoList; plu1546; -.
DR   eggNOG; COG0281; -.
DR   HOGENOM; HOG000042486; -.
DR   KO; K00027; -.
DR   OMA; IQDNNET; -.
DR   OrthoDB; EOG6QVRGM; -.
DR   GO; GO:0004471; F:malate dehydrogenase (decarboxylating) (NAD+) activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0008948; F:oxaloacetate decarboxylase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   Gene3D; 3.40.50.10380; -; 1.
DR   Gene3D; 3.40.50.720; -; 1.
DR   HAMAP; MF_01619; NAD_malic_enz; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N_dom.
DR   InterPro; IPR012302; Malic_NAD-bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR023667; NAD_malic_enz_proteobac.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PIRSF; PIRSF000106; ME; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   SMART; SM00919; Malic_M; 1.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN         1    565       NAD-dependent malic enzyme.
FT                                /FTId=PRO_0000160220.
FT   ACT_SITE    104    104       Proton donor. {ECO:0000255|HAMAP-
FT                                Rule:MF_01619}.
FT   ACT_SITE    175    175       Proton acceptor. {ECO:0000255|HAMAP-
FT                                Rule:MF_01619}.
FT   METAL       246    246       Divalent metal cation.
FT                                {ECO:0000255|HAMAP-Rule:MF_01619}.
FT   METAL       247    247       Divalent metal cation.
FT                                {ECO:0000255|HAMAP-Rule:MF_01619}.
FT   METAL       270    270       Divalent metal cation.
FT                                {ECO:0000255|HAMAP-Rule:MF_01619}.
FT   BINDING     157    157       NAD. {ECO:0000255|HAMAP-Rule:MF_01619}.
FT   BINDING     270    270       NAD. {ECO:0000255|HAMAP-Rule:MF_01619}.
FT   BINDING     418    418       NAD. {ECO:0000255|HAMAP-Rule:MF_01619}.
FT   SITE        270    270       Important for activity.
FT                                {ECO:0000255|HAMAP-Rule:MF_01619}.
SQ   SEQUENCE   565 AA;  63000 MW;  F15FE165CCAC9CC7 CRC64;
     MELEHESKRP LYIPYSGPIL LEFPLLNKGS AFTEEERRNF NLHGLLPEAV ETIEEQAERA
     YRQYLDFKND ADKHIYLRNI QDTNETLFYR LLDAHLNEMM PIIYTPTVGE ACEHFSDIYR
     RARGLFISHP NKAHIDDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT
     SCGGISPAYT LPVVLDVGTN NPQRLNDPLY MGWRHPRITG KEYDEFVDEF IQAVKRRWPN
     VLLQFEDFAQ KNAMPLLNRY RHEICCFNDD IQGTAAVTLG SLIAASRAAG RQLKDQTVTF
     LGAGSAGCGI AEQIVAQMKS EGLSDEQARA RIFMVDRFGL LTDKLPNLLD FQNKLVQKSS
     SLAKWDVNND SISLLDVVRN AKPTVLIGVS GQAGLFTEEI IREMHKHCER PIVMPLSNPT
     SRVEARPEDI INWTDGAALV ATGSPFNPVK YKDQEYPIAQ CNNAYIFPGI GLGVIASGAK
     LVTDGMLMAA SRTLANCSPL AQEGQGPLLP LIDDIQEVSR KIAKQVAKEA QIQGVATVTS
     DGALDEAIER NFWKPEYRVY KRTSF
//
DBGET integrated database retrieval system