ID MAO1_PHOLL Reviewed; 565 AA.
AC Q7N6K4;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 01-MAY-2013, entry version 67.
DE RecName: Full=NAD-dependent malic enzyme;
DE Short=NAD-ME;
DE EC=1.1.1.38;
GN Name=maeA; OrderedLocusNames=plu1546;
OS Photorhabdus luminescens subsp. laumondii (strain TT01).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Photorhabdus.
OX NCBI_TaxID=243265;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TT01;
RX PubMed=14528314; DOI=10.1038/nbt886;
RA Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S.,
RA Medigue C., Lanois A., Powell K., Siguier P., Vincent R., Wingate V.,
RA Zouine M., Glaser P., Boemare N., Danchin A., Kunst F.;
RT "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT luminescens.";
RL Nat. Biotechnol. 21:1307-1313(2003).
CC -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC -!- CATALYTIC ACTIVITY: Oxaloacetate = pyruvate + CO(2).
CC -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC (By similarity).
CC -!- SUBUNIT: Homotetramer (By similarity).
CC -!- SIMILARITY: Belongs to the malic enzymes family.
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DR EMBL; BX571864; CAE13839.1; -; Genomic_DNA.
DR RefSeq; NP_928837.1; NC_005126.1.
DR ProteinModelPortal; Q7N6K4; -.
DR STRING; 243265.plu1546; -.
DR PRIDE; Q7N6K4; -.
DR EnsemblBacteria; CAE13839; CAE13839; plu1546.
DR GeneID; 2801541; -.
DR KEGG; plu:plu1546; -.
DR PATRIC; 20506967; VBIPhoLum48522_1726.
DR GenoList; plu1546; -.
DR eggNOG; COG0281; -.
DR HOGENOM; HOG000042486; -.
DR KO; K00027; -.
DR OMA; HCERPIV; -.
DR ProtClustDB; PRK13529; -.
DR GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:HAMAP.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.10380; -; 1.
DR Gene3D; 3.40.50.720; -; 1.
DR HAMAP; MF_01619; NAD_malic_enz; 1; -.
DR InterPro; IPR015884; Malic_enzyme_CS.
DR InterPro; IPR012301; Malic_N.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR001891; Malic_OxRdtase.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR023667; NAD_malic_enz_proteobac.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR PIRSF; PIRSF000106; ME; 1.
DR PRINTS; PR00072; MALOXRDTASE.
DR SMART; SM00919; Malic_M; 1.
DR PROSITE; PS00331; MALIC_ENZYMES; 1.
PE 3: Inferred from homology;
KW Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT CHAIN 1 565 NAD-dependent malic enzyme.
FT /FTId=PRO_0000160220.
FT ACT_SITE 104 104 Proton donor (By similarity).
FT ACT_SITE 175 175 Proton acceptor (By similarity).
FT METAL 246 246 Divalent metal cation (By similarity).
FT METAL 247 247 Divalent metal cation (By similarity).
FT METAL 270 270 Divalent metal cation (By similarity).
FT BINDING 157 157 NAD (By similarity).
FT BINDING 270 270 NAD (By similarity).
FT BINDING 418 418 NAD (By similarity).
FT SITE 270 270 Important for activity (By similarity).
SQ SEQUENCE 565 AA; 63000 MW; F15FE165CCAC9CC7 CRC64;
MELEHESKRP LYIPYSGPIL LEFPLLNKGS AFTEEERRNF NLHGLLPEAV ETIEEQAERA
YRQYLDFKND ADKHIYLRNI QDTNETLFYR LLDAHLNEMM PIIYTPTVGE ACEHFSDIYR
RARGLFISHP NKAHIDDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT
SCGGISPAYT LPVVLDVGTN NPQRLNDPLY MGWRHPRITG KEYDEFVDEF IQAVKRRWPN
VLLQFEDFAQ KNAMPLLNRY RHEICCFNDD IQGTAAVTLG SLIAASRAAG RQLKDQTVTF
LGAGSAGCGI AEQIVAQMKS EGLSDEQARA RIFMVDRFGL LTDKLPNLLD FQNKLVQKSS
SLAKWDVNND SISLLDVVRN AKPTVLIGVS GQAGLFTEEI IREMHKHCER PIVMPLSNPT
SRVEARPEDI INWTDGAALV ATGSPFNPVK YKDQEYPIAQ CNNAYIFPGI GLGVIASGAK
LVTDGMLMAA SRTLANCSPL AQEGQGPLLP LIDDIQEVSR KIAKQVAKEA QIQGVATVTS
DGALDEAIER NFWKPEYRVY KRTSF
//
