ID Q7SXK3_DANRE Unreviewed; 538 AA.
AC Q7SXK3;
DT 01-OCT-2003, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2003, sequence version 1.
DT 27-MAR-2024, entry version 120.
DE RecName: Full=Pyruvate kinase {ECO:0000256|ARBA:ARBA00012142, ECO:0000256|RuleBase:RU000504};
DE EC=2.7.1.40 {ECO:0000256|ARBA:ARBA00012142, ECO:0000256|RuleBase:RU000504};
GN Name=pklr {ECO:0000313|EMBL:AAH55561.1,
GN ECO:0000313|RefSeq:NP_958446.1, ECO:0000313|ZFIN:ZDB-GENE-010907-1};
GN Synonyms=wu:fd15e01 {ECO:0000313|RefSeq:NP_958446.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AAH55561.1};
RN [1] {ECO:0000313|RefSeq:NP_958446.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958446.1};
RX PubMed=12853146; DOI=10.1016/S0378-1119(03)00580-8;
RA Sharma M.K., Denovan-Wright E.M., Boudreau M.E., Wright J.M.;
RT "A cellular retinoic acid-binding protein from zebrafish (Danio rerio):
RT cDNA sequence, phylogenetic analysis, mRNA expression, and gene linkage
RT mapping.";
RL Gene 311:119-128(2003).
RN [2] {ECO:0000313|EMBL:AAH55561.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB {ECO:0000313|EMBL:AAH55561.1};
RC TISSUE=Whole body {ECO:0000313|EMBL:AAH55561.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|RefSeq:NP_958446.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958446.1};
RX PubMed=16109975;
RA Woods I.G., Wilson C., Friedlander B., Chang P., Reyes D.K., Nix R.,
RA Kelly P.D., Chu F., Postlethwait J.H., Talbot W.S.;
RT "The zebrafish gene map defines ancestral vertebrate chromosomes.";
RL Genome Res. 15:1307-1314(2005).
RN [4] {ECO:0000313|RefSeq:NP_958446.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958446.1};
RX PubMed=16631158;
RA Cheng W., Guo L., Zhang Z., Soo H.M., Wen C., Wu W., Peng J.;
RT "HNF factors form a network to regulate liver-enriched genes in
RT zebrafish.";
RL Dev. Biol. 294:482-496(2006).
RN [5] {ECO:0000313|RefSeq:NP_958446.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958446.1};
RX PubMed=18602103;
RA Ruggeri B., Ubaldi M., Lourdusamy A., Soverchia L., Ciccocioppo R.,
RA Hardiman G., Baker M.E., Palermo F., Polzonetti-Magni A.M.;
RT "Variation of the genetic expression pattern after exposure to estradiol-
RT 17beta and 4-nonylphenol in male zebrafish (Danio rerio).";
RL Gen. Comp. Endocrinol. 158:138-144(2008).
RN [6] {ECO:0000313|RefSeq:NP_958446.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958446.1};
RX PubMed=17828398; DOI=10.1007/s00360-007-0201-4;
RA Marques I.J., Leito J.T., Spaink H.P., Testerink J., Jaspers R.T.,
RA Witte F., van den Berg S., Bagowski C.P.;
RT "Transcriptome analysis of the response to chronic constant hypoxia in
RT zebrafish hearts.";
RL J. Comp. Physiol. B 178:77-92(2008).
RN [7] {ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [8] {ECO:0000313|RefSeq:NP_958446.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958446.1};
RX PubMed=30248606;
RA Martinez R., Esteve-Codina A., Herrero-Nogareda L., Ortiz-Villanueva E.,
RA Barata C., Tauler R., Raldua D., Pina B., Navarro-Martin L.;
RT "Dose-dependent transcriptomic responses of zebrafish eleutheroembryos to
RT Bisphenol A.";
RL Environ. Pollut. 243:988-997(2018).
RN [9] {ECO:0000313|RefSeq:NP_958446.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958446.1};
RX PubMed=29867554;
RA Li J.M., Li L.Y., Qin X., Degrace P., Demizieux L., Limbu S.M., Wang X.,
RA Zhang M.L., Li D.L., Du Z.Y.;
RT "Inhibited Carnitine Synthesis Causes Systemic Alteration of Nutrient
RT Metabolism in Zebrafish.";
RL Front. Physiol. 9:509-509(2018).
RN [10] {ECO:0000313|RefSeq:NP_958446.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958446.1};
RX PubMed=30102881;
RA Gong Y., Zhai G., Su J., Yang B., Jin J., Liu H., Yin Z., Xie S., Han D.;
RT "Different roles of insulin receptor a and b in maintaining blood glucose
RT homeostasis in zebrafish.";
RL Gen. Comp. Endocrinol. 269:33-45(2018).
RN [11] {ECO:0000313|RefSeq:NP_958446.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958446.1};
RX PubMed=30448747;
RA Wan Z., Wang C., Zhou J., Shen M., Wang X., Fu Z., Jin Y.;
RT "Effects of polystyrene microplastics on the composition of the microbiome
RT and metabolism in larval zebrafish.";
RL Chemosphere 217:646-658(2019).
RN [12] {ECO:0000313|RefSeq:NP_958446.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958446.1};
RX PubMed=30471337;
RA Lin Y.S., Saputra F., Chen Y.C., Hu S.Y.;
RT "Dietary administration of Bacillus amyloliquefaciens R8 reduces hepatic
RT oxidative stress and enhances nutrient metabolism and immunity against
RT Aeromonas hydrophila and Streptococcus agalactiae in zebrafish (Danio
RT rerio).";
RL Fish Shellfish Immunol. 86:410-419(2019).
RN [13] {ECO:0000313|RefSeq:NP_958446.1}
RP IDENTIFICATION.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958446.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate;
CC Xref=Rhea:RHEA:18157, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC EC=2.7.1.40; Evidence={ECO:0000256|RuleBase:RU000504};
CC -!- COFACTOR:
CC Name=K(+); Xref=ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00001958};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 5/5. {ECO:0000256|ARBA:ARBA00004997,
CC ECO:0000256|RuleBase:RU000504}.
CC -!- SIMILARITY: Belongs to the pyruvate kinase family.
CC {ECO:0000256|ARBA:ARBA00008663, ECO:0000256|RuleBase:RU000504}.
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DR EMBL; BC055561; AAH55561.1; -; mRNA.
DR RefSeq; NP_958446.1; NM_201289.1.
DR GeneID; 114551; -.
DR KEGG; dre:114551; -.
DR AGR; ZFIN:ZDB-GENE-010907-1; -.
DR CTD; 5313; -.
DR ZFIN; ZDB-GENE-010907-1; pklr.
DR OrthoDB; 312683at2759; -.
DR PhylomeDB; Q7SXK3; -.
DR UniPathway; UPA00109; UER00188.
DR Proteomes; UP000000437; Chromosome 16.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0030955; F:potassium ion binding; IEA:InterPro.
DR GO; GO:0004743; F:pyruvate kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0001889; P:liver development; IMP:ZFIN.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00288; Pyruvate_Kinase; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 2.40.33.10; PK beta-barrel domain-like; 1.
DR Gene3D; 3.40.1380.20; Pyruvate kinase, C-terminal domain; 2.
DR InterPro; IPR001697; Pyr_Knase.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR InterPro; IPR011037; Pyrv_Knase-like_insert_dom_sf.
DR InterPro; IPR018209; Pyrv_Knase_AS.
DR InterPro; IPR015793; Pyrv_Knase_brl.
DR InterPro; IPR015795; Pyrv_Knase_C.
DR InterPro; IPR036918; Pyrv_Knase_C_sf.
DR InterPro; IPR015806; Pyrv_Knase_insert_dom_sf.
DR NCBIfam; TIGR01064; pyruv_kin; 1.
DR PANTHER; PTHR11817; PYRUVATE KINASE; 1.
DR PANTHER; PTHR11817:SF114; PYRUVATE KINASE; 1.
DR Pfam; PF00224; PK; 1.
DR Pfam; PF02887; PK_C; 1.
DR PRINTS; PR01050; PYRUVTKNASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF50800; PK beta-barrel domain-like; 1.
DR SUPFAM; SSF52935; PK C-terminal domain-like; 1.
DR PROSITE; PS00110; PYRUVATE_KINASE; 1.
PE 1: Evidence at protein level;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152, ECO:0000256|RuleBase:RU000504};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|RuleBase:RU000504};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU000504};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q7SXK3};
KW Pyruvate {ECO:0000256|ARBA:ARBA00023317, ECO:0000313|EMBL:AAH55561.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Transferase {ECO:0000256|RuleBase:RU000504}.
FT DOMAIN 49..382
FT /note="Pyruvate kinase barrel"
FT /evidence="ECO:0000259|Pfam:PF00224"
FT DOMAIN 419..533
FT /note="Pyruvate kinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02887"
SQ SEQUENCE 538 AA; 58945 MW; 92008E854DA9CA61 CRC64;
MGARIRRYSE VMALPDSFIQ KQQLDASMAD TFLEHLCLLD IDQEPITARN TSIICTIGPA
SRSITKLQEM VKAGMNIARL NFSHGTHQYH AETIRNVREA VETLTSDPLY YRPVAIALDT
KGPEIRTGLV KGRADAEVTL ERGALVRVVT AECEREQTDG SVIWMDYPSL TRVLKKDSRI
YIDDGLLALR VLEIGDSWLQ ARVENGGVLG SSKGVNLPGA ELLDLPAVSE RDRSDLQFGV
EQQVDMIFAS FIRCAEDVRA VRDALGPQGH DIKIISKVES RQGVRNFEQV LQESDGVMVA
RGDLGIEIPA EKVFIAQKMM IGRCNSAGKP VICATQMLES MVHHARPTRA ESSDVANAVL
DGADCVMLSG ETAKGHFPVE AVAMMHSICR EAEAAIFHQQ LFEELRRLTP LSSDPTEVTA
IGAVESSYKC CAGAIIILTT SGRSAQLLSR YRPRCPIIAV TRNAQVARQS QLLRGVFPAL
FRAPPAEVWA DDVDNRVTFA MDIGKARGFF RSGDMVIVVT GWSPGSGHTN IMRAVTVP
//
