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Database: UniProt
Entry: Q7TNG5
LinkDB: Q7TNG5
Original site: Q7TNG5 
ID   EMAL2_MOUSE             Reviewed;         649 AA.
AC   Q7TNG5; Q9DAS8;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   01-OCT-2014, entry version 94.
DE   RecName: Full=Echinoderm microtubule-associated protein-like 2;
DE            Short=EMAP-2;
GN   Name=Eml2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=C57BL/6J; TISSUE=Placenta;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=FVB/N; TISSUE=Colon;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Tubulin binding protein that inhibits microtubule
CC       nucleation and growth, resulting in shorter microtubules.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Binds unpolymerized tubulins via its WD repeat region.
CC       Interacts with GRID2 and may also interact with GRID1 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}.
CC       Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Note=Colocalizes
CC       with the microtubule cytoskeleton. Colocalizes with the mitotic
CC       spindle (By similarity). {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q7TNG5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q7TNG5-2; Sequence=VSP_024480;
CC         Note=No experimental confirmation available.;
CC   -!- DOMAIN: Contains a tandem atypical propeller in EMLs (TAPE)
CC       domain. The N-terminal beta-propeller is formed by canonical WD
CC       repeats; in contrast, the second beta-propeller contains one blade
CC       that is formed by discontinuous parts of the polypeptide chain (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WD repeat EMAP family. {ECO:0000305}.
CC   -!- SIMILARITY: Contains 11 WD repeats. {ECO:0000255|PROSITE-
CC       ProRule:PRU00221}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB24122.1; Type=Frameshift; Positions=32; Evidence={ECO:0000305};
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DR   EMBL; AK005562; BAB24122.1; ALT_FRAME; mRNA.
DR   EMBL; BC055476; AAH55476.1; -; mRNA.
DR   CCDS; CCDS52055.1; -. [Q7TNG5-2]
DR   RefSeq; NP_001156468.1; NM_001162996.1. [Q7TNG5-1]
DR   RefSeq; NP_082429.1; NM_028153.1. [Q7TNG5-2]
DR   UniGene; Mm.154121; -.
DR   ProteinModelPortal; Q7TNG5; -.
DR   SMR; Q7TNG5; 103-646.
DR   IntAct; Q7TNG5; 1.
DR   MINT; MINT-5085024; -.
DR   STRING; 10090.ENSMUSP00000112491; -.
DR   PhosphoSite; Q7TNG5; -.
DR   MaxQB; Q7TNG5; -.
DR   PaxDb; Q7TNG5; -.
DR   PRIDE; Q7TNG5; -.
DR   Ensembl; ENSMUST00000048502; ENSMUSP00000037654; ENSMUSG00000040811. [Q7TNG5-2]
DR   GeneID; 72205; -.
DR   KEGG; mmu:72205; -.
DR   UCSC; uc009fkz.2; mouse. [Q7TNG5-1]
DR   UCSC; uc009fla.2; mouse. [Q7TNG5-2]
DR   CTD; 24139; -.
DR   MGI; MGI:1919455; Eml2.
DR   eggNOG; COG2319; -.
DR   GeneTree; ENSGT00550000074369; -.
DR   HOGENOM; HOG000294061; -.
DR   HOVERGEN; HBG051470; -.
DR   InParanoid; Q7TNG5; -.
DR   TreeFam; TF317832; -.
DR   NextBio; 335691; -.
DR   PRO; PR:Q7TNG5; -.
DR   ArrayExpress; Q7TNG5; -.
DR   Bgee; Q7TNG5; -.
DR   CleanEx; MM_EML2; -.
DR   Genevestigator; Q7TNG5; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:Ensembl.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0015631; F:tubulin binding; ISS:UniProtKB.
DR   GO; GO:0031115; P:negative regulation of microtubule polymerization; ISS:UniProtKB.
DR   GO; GO:0010968; P:regulation of microtubule nucleation; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 3.
DR   InterPro; IPR005108; HELP.
DR   InterPro; IPR011047; Quinonprotein_ADH-like_supfam.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR017986; WD40_repeat_dom.
DR   Pfam; PF03451; HELP; 1.
DR   Pfam; PF00400; WD40; 4.
DR   SMART; SM00320; WD40; 11.
DR   SUPFAM; SSF50998; SSF50998; 5.
DR   PROSITE; PS50082; WD_REPEATS_2; 4.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton;
KW   Microtubule; Reference proteome; Repeat; WD repeat.
FT   CHAIN         1    649       Echinoderm microtubule-associated
FT                                protein-like 2.
FT                                /FTId=PRO_0000284388.
FT   REPEAT      103    152       WD 1.
FT   REPEAT      157    200       WD 2.
FT   REPEAT      203    242       WD 3.
FT   REPEAT      250    288       WD 4.
FT   REPEAT      292    331       WD 5.
FT   REPEAT      375    414       WD 6.
FT   REPEAT      416    455       WD 7.
FT   REPEAT      458    497       WD 8.
FT   REPEAT      504    543       WD 9.
FT   REPEAT      571    610       WD 10.
FT   REPEAT      616    649       WD 11.
FT   REGION       10    649       Tandem atypical propeller in EMLs.
FT                                {ECO:0000250}.
FT   VAR_SEQ      61     61       Y -> YPLTSSLSALLEEALGFSSY (in isoform 2).
FT                                {ECO:0000303|PubMed:16141072}.
FT                                /FTId=VSP_024480.
FT   CONFLICT    251    251       K -> R (in Ref. 1; BAB24122).
FT                                {ECO:0000305}.
SQ   SEQUENCE   649 AA;  70734 MW;  7B85AE89FE9ECAAC CRC64;
     MSSFGIGKTK EVIFSMEEGS VKMFLRGRPV PMLIPDELAP TYSLDTRSEL PSSRLKLDWV
     YGYRGRDCRA NLYLLPTGEV VYFVASVAVL YSVEEQRQRH YLGHNDDIKC LAVHPDMVTI
     ATGQVAGTTK EGKPLPPHVR VWDSVSLSTL HVLGLGVFDR AVCCVAFSKS NGGNLLCAVD
     ESNDHVLSVW DWAKESKVVD SKCSNEAVLV ATFHPTDPSL LITCGKSHIY FWSLEGGSLS
     KRQGLFEKHE KPKYVLCVTF LEGGDVVTGD SGGNLYVWGK GGNRITQEVQ GAHDGGVFAL
     CALRDGTLVS GGGRDRRVVL WGSDYSKVQE VEVPEDFGPV RTVAEGRGDT LYVGTTRNSI
     LLGSVHTGFS LLVQGHVEEL WGLATHPSRA QFVTCGQDKL VHLWSSETHQ PVWSRSIEDP
     ARSAGFHPSG SVLAVGTVTG RWLLLDTETH DLVAIHTDGN EQISVVSFSP DGAYLAVGSH
     DNLVYVYTVD QGGRKVSRLG KCSGHSSFIT HLDWAQDSTC FVTNSGDYEI LYWDPVTCKQ
     ITSADTVRNV EWATATCVLG FGVFGIWPEG ADGTDINAVA RSHDGKLLVS ADDFGKVHLF
     SYPCCQPRAL SHKYGGHSSH VTNVAFLWDD SMALTTGGKD TSVLQWRVA
//
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