ID GCP60_RAT Reviewed; 526 AA.
AC Q7TNY6;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-APR-2013, entry version 77.
DE RecName: Full=Golgi resident protein GCP60;
DE AltName: Full=Acyl-CoA-binding domain-containing protein 3;
DE AltName: Full=DMT1-associated protein;
DE Short=DAP;
DE AltName: Full=Golgi complex-associated protein 1;
DE Short=GOCAP1;
DE AltName: Full=Golgi phosphoprotein 1;
DE Short=GOLPH1;
GN Name=Acbd3; Synonyms=Gcp60;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muroidea; Muridae; Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Brown Norway;
RA Chen Y., Rodriguez-Paris J.M., Ma Y., Yeh M., Yeh K.-Y., Glass J.;
RT "Intestinal iron absorption: the interaction of DMT1 with DAP, an iron
RT responsive protein.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the maintenance of Golgi structure by
CC interacting with giantin, affecting protein transport between the
CC endoplasmic reticulum and Golgi. Involved in hormone-induced
CC steroid biosynthesis in testicular Leydig cells (By similarity).
CC -!- SUBUNIT: Interacts with the C-terminal cytoplasmic domain of
CC giantin/GOLGB1. Interacts with PBR and PKA regulatory subunit RI-
CC alpha. Does not interact with PKA regulatory subunit RI-beta nor
CC PKA regulatory subunit RII-alpha (By similarity).
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral
CC membrane protein; Cytoplasmic side (By similarity). Mitochondrion
CC (By similarity). Note=Also mitochondrial (via its interaction with
CC PBR) (By similarity).
CC -!- DOMAIN: The GOLD domain is essential for giantin binding (By
CC similarity).
CC -!- SIMILARITY: Contains 1 ACB (acyl-CoA-binding) domain.
CC -!- SIMILARITY: Contains 1 GOLD domain.
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DR EMBL; AY336075; AAP94639.1; -; mRNA.
DR IPI; IPI00339079; -.
DR RefSeq; NP_878263.1; NM_182843.1.
DR UniGene; Rn.57036; -.
DR ProteinModelPortal; Q7TNY6; -.
DR PhosphoSite; Q7TNY6; -.
DR PaxDb; Q7TNY6; -.
DR PRIDE; Q7TNY6; -.
DR GeneID; 289312; -.
DR KEGG; rno:289312; -.
DR UCSC; RGD:727851; rat.
DR CTD; 64746; -.
DR RGD; 727851; Acbd3.
DR eggNOG; NOG287879; -.
DR HOGENOM; HOG000247039; -.
DR HOVERGEN; HBG051712; -.
DR InParanoid; Q7TNY6; -.
DR OrthoDB; EOG4WH8M8; -.
DR NextBio; 629601; -.
DR Genevestigator; Q7TNY6; -.
DR GermOnline; ENSRNOG00000003185; Rattus norvegicus.
DR GO; GO:0000139; C:Golgi membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral to membrane; IEA:InterPro.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0000062; F:fatty-acyl-CoA binding; IEA:InterPro.
DR GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006810; P:transport; IEA:InterPro.
DR Gene3D; 1.20.80.10; -; 1.
DR InterPro; IPR022408; Acyl-CoA-binding_prot_CS.
DR InterPro; IPR000582; Acyl-CoA-binding_protein.
DR InterPro; IPR014352; FERM/acyl-CoA-bd_prot_3-hlx.
DR InterPro; IPR009038; GOLD.
DR Pfam; PF00887; ACBP; 1.
DR Pfam; PF13897; GOLD_2; 1.
DR SUPFAM; SSF47027; ACBP; 1.
DR SUPFAM; SSF101576; GOLD; 1.
DR PROSITE; PS00880; ACB_1; 1.
DR PROSITE; PS51228; ACB_2; 1.
DR PROSITE; PS50866; GOLD; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Complete proteome; Golgi apparatus; Lipid biosynthesis;
KW Lipid metabolism; Membrane; Mitochondrion; Phosphoprotein;
KW Reference proteome; Steroid biosynthesis.
FT INIT_MET 1 1 Removed (By similarity).
FT CHAIN 2 526 Golgi resident protein GCP60.
FT /FTId=PRO_0000214031.
FT DOMAIN 80 171 ACB.
FT DOMAIN 381 524 GOLD.
FT COILED 169 254 Potential.
FT COILED 450 471 Potential.
FT COMPBIAS 179 184 Poly-Glu.
FT COMPBIAS 186 235 Arg-rich.
FT COMPBIAS 238 305 Gln-rich.
FT MOD_RES 13 13 Phosphoserine (By similarity).
FT MOD_RES 20 20 Phosphoserine (By similarity).
FT MOD_RES 40 40 Phosphoserine (By similarity).
SQ SEQUENCE 526 AA; 60479 MW; FB1147D95DA48B45 CRC64;
MAAQLNVEQL EVSLDGLTLS PDSEERPGAE GAPLQTPPSS PPRDGLGSGT AGQQREPGEA
AAEGAAEEAR RMEQHWGFGL EELYGLALRF YKIKDGKAFH PTYEEKLKFV ALHKQVLLGP
YNPDTSPEVG FFDVLGNDRR REWAALGNMS KEDAMVEFVK LLNKCCPLLS AYVASHRIEK
EEEEKRRKAE EERRQREEEE RERLQKEEEK RKREEEDRLR REEEERRRIE EERLRLEQQK
QQIMAALNSQ TAVQFQQYAA QQYPGNYEQQ QILIRQLQEQ HYQQYMQQLY QVQLAQQQAA
LQKQQEVVVA GASLPASTKV NTAGASDPLP VNGQAKTHTE NPEKVLEPEA AEEALENGPK
DSLPVIAAPS MWTRPQIKDF KEKIRQDADS VITVRRGEVV TVRVPTHEEG SYLFWEFATD
SYDIGFGVYF EWTDSPNAAV SVHVSESSDD EEEEEEENVT CEEKAKKNVN KPLLDEIVPV
YRRDCHEEVY AGSHQYPGRG VYLLKFDNSY SLWRSKSVYY RVYYTR
//
