ID UVRB_HAEDU Reviewed; 675 AA.
AC Q7VLL3;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 29-MAY-2013, entry version 66.
DE RecName: Full=UvrABC system protein B;
DE Short=Protein UvrB;
DE AltName: Full=Excinuclease ABC subunit B;
GN Name=uvrB; OrderedLocusNames=HD_1416;
OS Haemophilus ducreyi (strain 35000HP / ATCC 700724).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=233412;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=35000HP / ATCC 700724;
RA Munson R.S. Jr., Ray W.C., Mahairas G., Sabo P., Mungur R.,
RA Johnson L., Nguyen D., Wang J., Forst C., Hood L.;
RT "The complete genome sequence of Haemophilus ducreyi.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. A damage recognition complex composed
CC of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon
CC binding of the UvrA(2)B(2) complex to a putative damaged site, the
CC DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP
CC binding by UvrB and probably causes local melting of the DNA
CC helix, facilitating insertion of UvrB beta-hairpin between the DNA
CC strands. Then UvrB probes one DNA strand for the presence of a
CC lesion. If a lesion is found the UvrA subunits dissociate and the
CC UvrB-DNA preincision complex is formed. This complex is
CC subsequently bound by UvrC and the second UvrB is released. If no
CC lesion is found, the DNA wraps around the other UvrB subunit that
CC will check the other stand for damage (By similarity).
CC -!- SUBUNIT: Forms a heterotetramer with UvrA during the search for
CC lesions. Interacts with UvrC in an incision complex (By
CC similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
CC similarity).
CC -!- SIMILARITY: Belongs to the UvrB family.
CC -!- SIMILARITY: Contains 1 helicase ATP-binding domain.
CC -!- SIMILARITY: Contains 1 helicase C-terminal domain.
CC -!- SIMILARITY: Contains 1 UVR domain.
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DR EMBL; AE017143; AAP96222.1; -; Genomic_DNA.
DR RefSeq; NP_873833.1; NC_002940.2.
DR ProteinModelPortal; Q7VLL3; -.
DR SMR; Q7VLL3; 8-599.
DR STRING; 233412.HD1416; -.
DR EnsemblBacteria; AAP96222; AAP96222; HD_1416.
DR GeneID; 1491302; -.
DR KEGG; hdu:HD1416; -.
DR PATRIC; 20179098; VBIHaeDuc133973_1183.
DR eggNOG; COG0556; -.
DR KO; K03702; -.
DR OMA; FTIAHVI; -.
DR ProtClustDB; PRK05298; -.
DR BioCyc; HDUC233412:GH5F-1280-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0003677; F:DNA binding; IEA:HAMAP.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:HAMAP.
DR GO; GO:0004386; F:helicase activity; IEA:HAMAP.
DR GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:HAMAP.
DR GO; GO:0009432; P:SOS response; IEA:HAMAP.
DR Gene3D; 4.10.860.10; -; 1.
DR HAMAP; MF_00204; UvrB; 1; -.
DR InterPro; IPR006935; Helicase/UvrB_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001943; UVR_dom.
DR InterPro; IPR004807; UvrB.
DR InterPro; IPR024759; UvrB_YAD/RRR_dom.
DR PANTHER; PTHR24029; PTHR24029; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF04851; ResIII; 1.
DR Pfam; PF02151; UVR; 1.
DR Pfam; PF12344; UvrB; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR SUPFAM; SSF46600; UvrB_C; 1.
DR TIGRFAMs; TIGR00631; uvrb; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50151; UVR; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA excision;
KW DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
FT CHAIN 1 675 UvrABC system protein B.
FT /FTId=PRO_0000138394.
FT DOMAIN 29 414 Helicase ATP-binding.
FT DOMAIN 434 600 Helicase C-terminal.
FT DOMAIN 636 671 UVR.
FT NP_BIND 42 49 ATP (Potential).
FT MOTIF 95 118 Beta-hairpin.
SQ SEQUENCE 675 AA; 76609 MW; 71F2C9760DEFE2D6 CRC64;
MSKPGKPFIL NSPFAPSGDQ PSAIKKLTEG LQDGLAHQTL LGVTGSGKTF TIANVIAQLN
RPAMLLAPNK TLAAQLYAEM KAFFPENAVE YFVSYYDYYQ PEAYVPASDT FIEKDASINE
QIEQMRLSAT KSFLERRDTI VVASVSAIYG LGDVNAYMQM MLHLQVGAII NQRDILARLA
ELQYTRNDQA FQRSTFRVRG EVIDIFPAES DEIALRIELF DDEIDNLSLF DPLTGHSFGK
IPRYTIYPKT HYVTPRERIL TAIDQIKNEL TGRQDYFIKE HKLLEEQRIT QRTQFDIEMI
NELGYCSGIE NYSRYLSGRQ EGEPPPTLFD YMPADGLLII DESHVTVPQI GGMYRGDRAR
KETLVQYGFR LPSALDNRPL KFEEFERLSP QTIYVSATPG NYELEKSNGD IIDQVVRPTG
LLDPIIEVRP VASQVDDVLS EIQQRVALDE RVLITTLTKK MAEDLTDYLD EHGVRVRYLH
SDIDTVERVE IIHDLRIGMF DVLVGINLLR EGLDMPEVAL VAILDADKEG FLRSESSLIQ
TIGRAARNLN GKAILYADRI THSMQKAITE TERRREKQQK YNQEHNIIPQ ALNKKVRELL
DIGQTDKQKK GKQAVKIAEQ SASYTPNAPK SRQALEKELK QLTQQMQKLA KDLEFEKAAA
VRDKIGQLKM ALLEV
//
