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Database: UniProt
Entry: Q7X7E9
LinkDB: Q7X7E9
Original site: Q7X7E9 
ID   DNLI4_ORYSJ             Reviewed;        1322 AA.
AC   Q7X7E9;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 2.
DT   30-AUG-2017, entry version 70.
DE   RecName: Full=Putative DNA ligase 4;
DE            EC=6.5.1.1 {ECO:0000255|PROSITE-ProRule:PRU10135};
DE   AltName: Full=DNA ligase IV;
DE   AltName: Full=Polydeoxyribonucleotide synthase [ATP] 4;
GN   Name=LIG4; OrderedLocusNames=Os04g0606450, LOC_Os04g51700;
GN   ORFNames=OSJNBa0035M09.8;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J.,
RA   Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y.,
RA   Weng Q., Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D.,
RA   Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J.,
RA   Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H.,
RA   Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y.,
RA   Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W.,
RA   Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W.,
RA   Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y.,
RA   Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
RA   McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
RA   Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
RA   Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
RA   Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using
RT   next generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1068-1322.
RC   STRAIN=cv. Nipponbare;
RG   The rice full-length cDNA consortium;
RT   "Oryza sativa full length cDNA.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n)-3'-hydroxyl +
CC       5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) +
CC       AMP + diphosphate. {ECO:0000255|PROSITE-ProRule:PRU10135}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
CC       {ECO:0000305}.
DR   EMBL; AL662968; CAD41778.2; -; Genomic_DNA.
DR   EMBL; AP014960; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK242148; -; NOT_ANNOTATED_CDS; mRNA.
DR   ProteinModelPortal; Q7X7E9; -.
DR   PRIDE; Q7X7E9; -.
DR   InParanoid; Q7X7E9; -.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; Q7X7E9; OS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0032807; C:DNA ligase IV complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051103; P:DNA ligation involved in DNA repair; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IBA:GO_Central.
DR   GO; GO:0006297; P:nucleotide-excision repair, DNA gap filling; IBA:GO_Central.
DR   CDD; cd00027; BRCT; 1.
DR   Gene3D; 1.10.3260.10; -; 1.
DR   Gene3D; 3.40.50.10190; -; 2.
DR   InterPro; IPR001357; BRCT_dom.
DR   InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR   InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR   InterPro; IPR016059; DNA_ligase_ATP-dep_CS.
DR   InterPro; IPR012308; DNA_ligase_ATP-dep_N.
DR   InterPro; IPR021536; DNA_ligase_IV_dom.
DR   InterPro; IPR029710; LIG4.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   PANTHER; PTHR10459:SF84; PTHR10459:SF84; 1.
DR   Pfam; PF16589; BRCT_2; 1.
DR   Pfam; PF04679; DNA_ligase_A_C; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   Pfam; PF04675; DNA_ligase_A_N; 1.
DR   Pfam; PF11411; DNA_ligase_IV; 1.
DR   SUPFAM; SSF117018; SSF117018; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF52113; SSF52113; 2.
DR   PROSITE; PS50172; BRCT; 2.
DR   PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR   PROSITE; PS00333; DNA_LIGASE_A2; 1.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Complete proteome; DNA damage; DNA recombination;
KW   DNA repair; DNA replication; Ligase; Magnesium; Metal-binding;
KW   Nucleotide-binding; Nucleus; Reference proteome; Repeat.
FT   CHAIN         1   1322       Putative DNA ligase 4.
FT                                /FTId=PRO_0000247468.
FT   DOMAIN      686    768       BRCT 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00033}.
FT   DOMAIN      825    935       BRCT 2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00033}.
FT   ACT_SITE    267    267       N6-AMP-lysine intermediate.
FT                                {ECO:0000255|PROSITE-ProRule:PRU10135}.
FT   METAL       317    317       Magnesium 1. {ECO:0000255}.
FT   METAL       497    497       Magnesium 2. {ECO:0000255}.
FT   BINDING     265    265       ATP. {ECO:0000250}.
FT   BINDING     272    272       ATP. {ECO:0000250}.
FT   BINDING     287    287       ATP. {ECO:0000250}.
FT   BINDING     502    502       ATP. {ECO:0000250}.
FT   BINDING     513    513       ATP. {ECO:0000250}.
FT   BINDING     519    519       ATP. {ECO:0000250}.
SQ   SEQUENCE   1322 AA;  149078 MW;  110BF7B94DB62A67 CRC64;
     MAATVRFGLL VAMFQAMSRD RTAAKKRARL RALLDRAYGP GGRDDYFSAL RLVLPGLDRE
     RGSYGLKEAA LASVLVDALG IAKDSPDAVR LTNWRRGGGF RNAGNFALVA AEVLQRRQGM
     TSGGLTIKEV NDALDRLAAT ENSVCLESEK LLLILDRSEK ASILSSLIKK TNALEMKWLL
     MIILKDLKLG ISEKSVFHEF HPDAEDLFNV TCDLRLVCEK LNDRSQRHKR QDIEVGKAVR
     PQLSMRVNNA SSAWKKLHGK QVVAECKFDG DRIQIHKNGE EIHFFSRSFL DHSEYAPGMS
     KVIIENILVD RCILDGEMLV WDTVLNRFAE FGSNQEIAKA AKEGLETDRQ LCCILEWIAS
     YVLALCVNIL SCQDFFFSNK RESYVAFDIL YAGDTSVIHQ SLTERHEILQ KVVRPLKGHL
     EILVPTGGLN IHRPPDEPCW SILAHSLDDV EKFFKDTVDN RFFPTSPNTD ELSNLSISLM
     IQFRFSDYSI CYVLREEGII LKDLESKWEP GDRSGKWLKL KPDYIHAGAD LDVIIIGGYY
     GSGRRGGEVA QFLVGLAVPS DDNSYPKRFL SFCRVGTGLS DEELDALVTK LKPHFRKNEY
     PKKPPRFYEV TNHSKERPDV WIESPDKQSV RFRKHLIYCI TKCRSVIISI TSDIRTIKSE
     VFAAPYSLRF PRIQRLRYDK PWHECLDVQA FVDIVHSSNG TTHRAADDDN DLKNVKNGGS
     FSMNLNDSVT HCIAAEKKAT RQGRIIHYSW ILDCCKEKRL LHLQPKFDPC LQPMHKFPEE
     IDSYADYFYW DIDISDLKQI FSNMDRAVVD SNMVHHYKKK YCADERFCFF QGCCVYFYHA
     PLVNADYNVI SDLALKRVKQ DLTMHGGQVC SILAPATHLI IVSVLQAYNF DMLYKSLPPA
     ERRYLHDKRL QVVSNKWLED SVEKQTRLPE TTYSLKPDTL EEIEIERSEE TVQPCNDKLE
     ENEKADTSHV KHAPRKRGRP SSSASRTAKP APRPVRRTRA RRGNQHAKID DVEPEESDHG
     ETGLDDQIPD TDNISKMEVD SFDKDQVSAR PVRRTRARRG KQHAKIDYGQ SEESDPGETG
     QDDQRLDADY ISKMEEDSSD RDQGAHPTAP RVVRRSRAQR GKWLAKIDRE TGPGETGQDD
     KKLNADSISK MEEHAHDKDQ EPPPGAQLIT LDEQEPKGIK SSTTETPSSP KHERNQTVLR
     RDTAETTSSA TCEKMEQMVD PLHAMLLDMI PSLGQMKTDV GNRVAEAKAE TNPPWVGSST
     SSYVAPVPQA SASSASSSGV PAPHAGSSTQ STGVPAPDPT AGAPKKKKVS YKDVAGALLK
     DW
//
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