ID Q804J6_DANRE Unreviewed; 1312 AA.
AC Q804J6;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 158.
DE RecName: Full=1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma {ECO:0000256|PIRNR:PIRNR000952};
DE EC=3.1.4.11 {ECO:0000256|PIRNR:PIRNR000952};
GN Name=plcg1 {ECO:0000313|EMBL:AAO23907.1,
GN ECO:0000313|ZFIN:ZDB-GENE-030421-3};
GN Synonyms=fj46f04 {ECO:0000313|RefSeq:NP_919388.1}, wu:fj46f04
GN {ECO:0000313|RefSeq:NP_919388.1}, wu:fj57a05
GN {ECO:0000313|RefSeq:NP_919388.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AAO23907.1};
RN [1] {ECO:0000313|RefSeq:NP_919388.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=11997344;
RA Smith S.F., Snell P., Gruetzner F., Bench A.J., Haaf T., Metcalfe J.A.,
RA Green A.R., Elgar G.;
RT "Analyses of the extent of shared synteny and conserved gene orders between
RT the genome of Fugu rubripes and human 20q.";
RL Genome Res. 12:776-784(2002).
RN [2] {ECO:0000313|EMBL:AAO23907.1, ECO:0000313|RefSeq:NP_919388.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=12782653; DOI=10.1101/gad.1072203;
RA Lawson N.D., Mugford J.W., Diamond B.A., Weinstein B.M.;
RT "phospholipase C gamma-1 is required downstream of vascular endothelial
RT growth factor during arterial development.";
RL Genes Dev. 17:1346-1351(2003).
RN [3] {ECO:0000313|RefSeq:NP_919388.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=15256591; DOI=10.1073/pnas.0403929101;
RA Amsterdam A., Nissen R.M., Sun Z., Swindell E.C., Farrington S.,
RA Hopkins N.;
RT "Identification of 315 genes essential for early zebrafish development.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:12792-12797(2004).
RN [4] {ECO:0000313|RefSeq:NP_919388.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=15998812; DOI=10.1101/gad.1319405;
RA Rottbauer W., Just S., Wessels G., Trano N., Most P., Katus H.A.,
RA Fishman M.C.;
RT "VEGF-PLCgamma1 pathway controls cardiac contractility in the embryonic
RT heart.";
RL Genes Dev. 19:1624-1634(2005).
RN [5] {ECO:0000313|RefSeq:NP_919388.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=16565716;
RA Bayliss P.E., Bellavance K.L., Whitehead G.G., Abrams J.M., Aegerter S.,
RA Robbins H.S., Cowan D.B., Keating M.T., O'Reilly T., Wood J.M.,
RA Roberts T.M., Chan J.;
RT "Chemical modulation of receptor signaling inhibits regenerative
RT angiogenesis in adult zebrafish.";
RL Nat. Chem. Biol. 2:265-273(2006).
RN [6] {ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [7] {ECO:0000313|RefSeq:NP_919388.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27789478;
RA Chen J., Zhu R.F., Li F.F., Liang Y.L., Wang C., Qin Y.W., Huang S.,
RA Zhao X.X., Jing Q.;
RT "MicroRNA-126a Directs Lymphangiogenesis Through Interacting With Chemokine
RT and Flt4 Signaling in Zebrafish.";
RL Arterioscler. Thromb. Vasc. Biol. 36:2381-2393(2016).
RN [8] {ECO:0000313|RefSeq:NP_919388.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27499523;
RA Monteiro R., Pinheiro P., Joseph N., Peterkin T., Koth J., Repapi E.,
RA Bonkhofer F., Kirmizitas A., Patient R.;
RT "Transforming Growth Factor beta Drives Hemogenic Endothelium Programming
RT and the Transition to Hematopoietic Stem Cells.";
RL Dev. Cell 38:358-370(2016).
RN [9] {ECO:0000313|RefSeq:NP_919388.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27499207;
RA Gao J., Mahapatra C.T., Mapes C.D., Khlebnikova M., Wei A., Sepulveda M.S.;
RT "Vascular toxicity of silver nanoparticles to developing zebrafish (Danio
RT rerio).";
RL Nanotoxicology 10:1363-1372(2016).
RN [10] {ECO:0000313|RefSeq:NP_919388.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=28179432;
RA Karpanen T., Padberg Y., van de Pavert S.A., Dierkes C., Morooka N.,
RA Peterson-Maduro J., van de Hoek G., Adrian M., Mochizuki N., Sekiguchi K.,
RA Kiefer F., Schulte D., Schulte-Merker S.;
RT "An Evolutionarily Conserved Role for Polydom/Svep1 During Lymphatic Vessel
RT Formation.";
RL Circ. Res. 120:1263-1275(2017).
RN [11] {ECO:0000313|RefSeq:NP_919388.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=30237797;
RA Nayak S., Khozin-Goldberg I., Cohen G., Zilberg D.;
RT "Dietary Supplementation With omega6 LC-PUFA-Rich Algae Modulates Zebrafish
RT Immune Function and Improves Resistance to Streptococcal Infection.";
RL Front. Immunol. 9:1960-1960(2018).
RN [12] {ECO:0000313|RefSeq:NP_919388.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Mediates the production of the second messenger molecules
CC diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an
CC important role in the regulation of intracellular signaling cascades.
CC {ECO:0000256|PIRNR:PIRNR000952}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-
CC bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-
CC diacyl-sn-glycerol + H(+); Xref=Rhea:RHEA:33179, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17815, ChEBI:CHEBI:58456,
CC ChEBI:CHEBI:203600; EC=3.1.4.11;
CC Evidence={ECO:0000256|ARBA:ARBA00023674};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33180;
CC Evidence={ECO:0000256|ARBA:ARBA00023674};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR EMBL; AY163168; AAO23907.1; -; mRNA.
DR RefSeq; NP_919388.1; NM_194407.1.
DR GeneID; 373867; -.
DR KEGG; dre:373867; -.
DR AGR; ZFIN:ZDB-GENE-030421-3; -.
DR CTD; 5335; -.
DR ZFIN; ZDB-GENE-030421-3; plcg1.
DR OrthoDB; 2900494at2759; -.
DR PhylomeDB; Q804J6; -.
DR Proteomes; UP000000437; Chromosome 23.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004435; F:phosphatidylinositol phospholipase C activity; IBA:GO_Central.
DR GO; GO:0001525; P:angiogenesis; IMP:ZFIN.
DR GO; GO:0048844; P:artery morphogenesis; IMP:ZFIN.
DR GO; GO:0031101; P:fin regeneration; IMP:ZFIN.
DR GO; GO:0030851; P:granulocyte differentiation; IMP:ZFIN.
DR GO; GO:0060218; P:hematopoietic stem cell differentiation; IMP:ZFIN.
DR GO; GO:0048015; P:phosphatidylinositol-mediated signaling; IBA:GO_Central.
DR GO; GO:0009395; P:phospholipid catabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0010634; P:positive regulation of epithelial cell migration; IBA:GO_Central.
DR GO; GO:0035477; P:regulation of angioblast cell migration involved in selective angioblast sprouting; IMP:ZFIN.
DR GO; GO:0008016; P:regulation of heart contraction; IMP:ZFIN.
DR GO; GO:0051209; P:release of sequestered calcium ion into cytosol; IBA:GO_Central.
DR GO; GO:0001570; P:vasculogenesis; IMP:ZFIN.
DR CDD; cd00275; C2_PLC_like; 1.
DR CDD; cd16214; EFh_PI-PLCgamma1; 1.
DR CDD; cd13362; PH_PLC_gamma; 1.
DR CDD; cd13234; PHsplit_PLC_gamma; 1.
DR CDD; cd08592; PI-PLCc_gamma; 1.
DR CDD; cd09932; SH2_C-SH2_PLC_gamma_like; 1.
DR CDD; cd10341; SH2_N-SH2_PLC_gamma_like; 1.
DR CDD; cd11970; SH3_PLCgamma1; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 3.20.20.190; Phosphatidylinositol (PI) phosphodiesterase; 2.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR Gene3D; 3.30.505.10; SH2 domain; 2.
DR Gene3D; 2.30.30.40; SH3 Domains; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR001192; PI-PLC_fam.
DR InterPro; IPR016279; PLC-gamma.
DR InterPro; IPR035023; PLC-gamma_C-SH2.
DR InterPro; IPR035024; PLC-gamma_N-SH2.
DR InterPro; IPR017946; PLC-like_Pdiesterase_TIM-brl.
DR InterPro; IPR035724; PLCgamma1_SH3.
DR InterPro; IPR000909; PLipase_C_PInositol-sp_X_dom.
DR InterPro; IPR001711; PLipase_C_Pinositol-sp_Y.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR PANTHER; PTHR10336:SF173; 1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE GAMMA-1; 1.
DR PANTHER; PTHR10336; PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF00169; PH; 2.
DR Pfam; PF00388; PI-PLC-X; 1.
DR Pfam; PF00387; PI-PLC-Y; 1.
DR Pfam; PF00017; SH2; 2.
DR Pfam; PF00018; SH3_1; 1.
DR PIRSF; PIRSF000952; PLC-gamma; 1.
DR PRINTS; PR00390; PHPHLIPASEC.
DR PRINTS; PR00401; SH2DOMAIN.
DR SMART; SM00239; C2; 1.
DR SMART; SM00233; PH; 3.
DR SMART; SM00148; PLCXc; 1.
DR SMART; SM00149; PLCYc; 1.
DR SMART; SM00252; SH2; 2.
DR SMART; SM00326; SH3; 1.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF50729; PH domain-like; 2.
DR SUPFAM; SSF51695; PLC-like phosphodiesterases; 1.
DR SUPFAM; SSF55550; SH2 domain; 2.
DR SUPFAM; SSF50044; SH3-domain; 1.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50003; PH_DOMAIN; 2.
DR PROSITE; PS50007; PIPLC_X_DOMAIN; 1.
DR PROSITE; PS50008; PIPLC_Y_DOMAIN; 1.
DR PROSITE; PS50001; SH2; 2.
DR PROSITE; PS50002; SH3; 1.
PE 1: Evidence at protein level;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR000952, ECO:0000256|RuleBase:RU361133};
KW Lipid degradation {ECO:0000256|PIRNR:PIRNR000952,
KW ECO:0000256|RuleBase:RU361133};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098,
KW ECO:0000256|PIRNR:PIRNR000952};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q804J6};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW SH2 domain {ECO:0000256|ARBA:ARBA00022999, ECO:0000256|PROSITE-
KW ProRule:PRU00191};
KW SH3 domain {ECO:0000256|ARBA:ARBA00022443, ECO:0000256|PROSITE-
KW ProRule:PRU00192};
KW Transducer {ECO:0000256|ARBA:ARBA00023224, ECO:0000256|PIRNR:PIRNR000952}.
FT DOMAIN 27..142
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 545..652
FT /note="SH2"
FT /evidence="ECO:0000259|PROSITE:PS50001"
FT DOMAIN 663..751
FT /note="SH2"
FT /evidence="ECO:0000259|PROSITE:PS50001"
FT DOMAIN 786..846
FT /note="SH3"
FT /evidence="ECO:0000259|PROSITE:PS50002"
FT DOMAIN 866..926
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 948..1065
FT /note="PI-PLC Y-box"
FT /evidence="ECO:0000259|PROSITE:PS50008"
FT DOMAIN 1066..1189
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT REGION 1241..1271
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1241..1261
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1224
FT /note="Phosphoserine"
FT /evidence="ECO:0000256|PIRSR:PIRSR000952-1"
FT MOD_RES 1274
FT /note="Phosphoserine"
FT /evidence="ECO:0000256|PIRSR:PIRSR000952-1"
SQ SEQUENCE 1312 AA; 151091 MW; A96DF520B2701896 CRC64;
MAASAGFSSN GPVPWALSDT EVNNLYRDLE LGTVLTLYYS KKSQRPERRT FQVKLETRQI
IWTRGTDKIE GEIDIREIKE IRTGQKSRDF ERYVEDVTTR PDQAHCFVIL YGTEFRLKSL
SLAATSDEEM VMWVKGLTWL MSDTLSSSTP LQIERWLRKQ FYAVDRNRED RISCKDLKCM
LCQVNYRVPN MKFLREKLPD GELRNGDVSY SHFAQLYRSL MFDAQKSMEI PLLQRFIDRP
EQKISLEDFQ NFLIEQQKEL WAADSNKVQE YMFRYLKDPL REIEQPYFHQ DEFLTYLFSK
ENTIWDAQLD QVRPEDMNNP LSHYWISSSH NTYLTGDQFS SESSLEAYAR CLRMGCRCIE
LDCWDGPDGM PVIYHGHTLT TKIKFSDVLN TIKEHAFVTS DYPIILSIED HCSIVQQRNM
ATFFKKVFGE MLLTKAVDIS ADGLPSPHQL KRKILIKHKK LAEGSAYEEV SSSTPYSEND
ISNSIKNGIL YLEDPINHEW YPHFFVLTSN KIYYSEETSS NQGNEDEEEH REICNGMDQH
VTEKWFHGKL GAGRDGRQIA ERLLSEYCVE TGAPDGSFLV RESETFVGDY TLSFWRSGRV
QHCRIHSRQE AGSPKFYLTD NLVFDTLFAL ITHYQQVPLR CNEFEMKLTE PVPQTNAHES
KEWYHACLSR SQAENMLMRV PRDGAFLVRK RTEFNSFAIS FRAEGKIKHC RVQQEGQTVV
LGTSEFDSLV DLISYYEKHP LYRKMKLRYP INEETLEKIG TAEPDYGSLY EGRNPGFYVE
ANQMPTFKCT VKAMYEYKAQ RDDELSFIKN AIISNVDKQE GGWWKGDCGG KKQLWFPANY
VEEISPTAVE PDRSASENSP LGDLLRGSVD VSSCQIVVRP EGKGSRQFVF SLVPVASPRT
GPVLDIAASS QEELKDWVVK IREVTMTSEA KLEEGKMMER RKKIALELSD LVIYCRPVPF
DEDKIGTERA CFRDMSSFPE TKAEKYVNRV KGKKFLQYNR LQLSRIYPRG QRLDSSNYDP
LPMWLCGSQL VALNFQTADK PMQMNQAIFM LNGRSGYVLQ PPIMRDDNFD PFDRHSLRGV
EPITLSIEVL GARHLPKHGR GIVCPLIEIE VCGAEYDNAK QRTDSEADNG LNPTWTRKLF
RFTVCNPSFA FLRFVVYEID MFSDQNFLAQ ATFPINCLKT GYRSVPLKNS YSEDLELACL
LVHMDVIRGR VGYEMIHENG DMLSPFSPAG AMAMAMQTGR ERMNDSGSVS STSSMSPLPS
SPAQALGYRG REGSFEARYQ TPLEDFRVSQ EALLDHENRR LRRARVSADN RA
//
