ID MAST4_MOUSE Reviewed; 2618 AA.
AC Q811L6; E9QAH7; Q3UVE7; Q62489; Q6ZQE0; Q8BME3;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-APR-2013, entry version 89.
DE RecName: Full=Microtubule-associated serine/threonine-protein kinase 4;
DE EC=2.7.11.1;
GN Name=Mast4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muroidea; Muridae; Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, Cerebellum, and Wolffian duct;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S.,
RA She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W.,
RA Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T.,
RA Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J.,
RA Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z.,
RA Lindblad-Toh K., Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of
RT the mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 346-2618 (ISOFORM 2).
RC STRAIN=Czech II; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1671-2618 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene:
RT III. The complete nucleotide sequences of 500 mouse KIAA-homologous
RT cDNAs identified by screening of terminal sequences of cDNA clones
RT randomly sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 2398-2498 (ISOFORM 1).
RC TISSUE=Cochlea;
RX PubMed=9119401; DOI=10.1006/geno.1996.4526;
RA Crozet F., El-Amraoui A., Blanchard S., Lenoir M., Ripoll C., Vago P.,
RA Hamel C., Fizames C., Levi-Acobas F., Depetris D., Mattei M.-G.,
RA Weil D., Pujol R., Petit C.;
RT "Cloning of the genes encoding two murine and human cochlear
RT unconventional type I myosins.";
RL Genomics 40:332-341(1997).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1521, AND MASS
RP SPECTROMETRY.
RC TISSUE=Melanoma;
RX PubMed=19367708; DOI=10.1021/pr800599n;
RA Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,
RA Faessler R., Mann M.;
RT "Solid tumor proteome and phosphoproteome analysis by high resolution
RT mass spectrometry.";
RL J. Proteome Res. 7:5314-5326(2008).
CC -!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
CC -!- COFACTOR: Magnesium (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q811L6-1; Sequence=Displayed;
CC Note=No experimental confirmation available;
CC Name=2;
CC IsoId=Q811L6-2; Sequence=VSP_041616, VSP_052478, VSP_052479,
CC VSP_052480;
CC Note=No experimental confirmation available;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr
CC protein kinase family.
CC -!- SIMILARITY: Contains 1 AGC-kinase C-terminal domain.
CC -!- SIMILARITY: Contains 1 PDZ (DHR) domain.
CC -!- SIMILARITY: Contains 1 protein kinase domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK090136; Type=Frameshift; Positions=1682;
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DR EMBL; AK032755; BAC28008.1; -; mRNA.
DR EMBL; AK090136; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK137360; BAE23322.1; -; mRNA.
DR EMBL; AC112791; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC114827; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC124387; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC161446; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC165290; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC167975; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC042511; AAH42511.1; -; mRNA.
DR EMBL; AK129114; BAC97924.1; -; mRNA.
DR EMBL; Z78145; CAB01547.1; -; mRNA.
DR IPI; IPI00346104; -.
DR IPI; IPI00465600; -.
DR RefSeq; NP_780380.2; NM_175171.3.
DR UniGene; Mm.202606; -.
DR UniGene; Mm.447520; -.
DR HSSP; P31751; 1MRY.
DR ProteinModelPortal; Q811L6; -.
DR SMR; Q811L6; 379-479, 1138-1229.
DR PhosphoSite; Q811L6; -.
DR PaxDb; Q811L6; -.
DR PRIDE; Q811L6; -.
DR Ensembl; ENSMUST00000167058; ENSMUSP00000128464; ENSMUSG00000034751.
DR GeneID; 328329; -.
DR KEGG; mmu:328329; -.
DR UCSC; uc007rsa.1; mouse.
DR UCSC; uc007rsb.2; mouse.
DR CTD; 375449; -.
DR MGI; MGI:1918885; Mast4.
DR eggNOG; COG0515; -.
DR GeneTree; ENSGT00530000063286; -.
DR HOGENOM; HOG000046662; -.
DR HOVERGEN; HBG108118; -.
DR InParanoid; Q811L6; -.
DR KO; K08789; -.
DR OMA; ECLPGNP; -.
DR OrthoDB; EOG4255S2; -.
DR NextBio; 398238; -.
DR ArrayExpress; Q811L6; -.
DR Bgee; Q811L6; -.
DR Genevestigator; Q811L6; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1480.20; -; 1.
DR InterPro; IPR000961; AGC-kinase_C.
DR InterPro; IPR011009; Kinase-like_dom.
DR InterPro; IPR015022; MA_Ser/Thr_Kinase_dom.
DR InterPro; IPR023142; MAST_pre-PK_dom.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR000719; Prot_kinase_cat_dom.
DR InterPro; IPR002290; Ser/Thr_dual-sp_kinase_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF08926; DUF1908; 1.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00133; S_TK_X; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; Kinase_like; 1.
DR SUPFAM; SSF50156; PDZ; 1.
DR SUPFAM; SSF140482; SSF140482; 1.
DR PROSITE; PS51285; AGC_KINASE_CTER; 1.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; FALSE_NEG.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Complete proteome; Cytoplasm;
KW Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Serine/threonine-protein kinase; Transferase.
FT CHAIN 1 2618 Microtubule-associated serine/threonine-
FT protein kinase 4.
FT /FTId=PRO_0000293629.
FT DOMAIN 568 841 Protein kinase.
FT DOMAIN 842 904 AGC-kinase C-terminal.
FT DOMAIN 1139 1227 PDZ.
FT NP_BIND 574 582 ATP (By similarity).
FT ACT_SITE 691 691 Proton acceptor (By similarity).
FT BINDING 597 597 ATP (By similarity).
FT MOD_RES 1521 1521 Phosphoserine.
FT VAR_SEQ 350 521 Missing (in isoform 2).
FT /FTId=VSP_041616.
FT VAR_SEQ 1030 1096 Missing (in isoform 2).
FT /FTId=VSP_052478.
FT VAR_SEQ 1263 1282 SLFKKLAKQPSPLLHTSRSF -> DRKKKKKRELSSRCLPS
FT SNR (in isoform 2).
FT /FTId=VSP_052479.
FT VAR_SEQ 1283 2618 Missing (in isoform 2).
FT /FTId=VSP_052480.
FT CONFLICT 546 546 M -> T (in Ref. 3; AAH42511).
FT CONFLICT 1963 1963 A -> T (in Ref. 4; BAC97924).
FT CONFLICT 1977 1977 K -> I (in Ref. 1; BAC28008).
FT CONFLICT 2143 2143 S -> G (in Ref. 1; BAC28008).
FT CONFLICT 2181 2181 A -> ATA (in Ref. 4; BAC97924).
FT CONFLICT 2444 2444 S -> G (in Ref. 1; BAC28008).
FT CONFLICT 2507 2507 D -> E (in Ref. 1; BAE23322).
SQ SEQUENCE 2618 AA; 284004 MW; 493D6B86DA86BA5C CRC64;
MGEKVSEAPE PVPRGCSGHG ARTLVSSAAA VSSEGASSAE SSSGSETLSE EGEPSRFSCR
SQPPRPPGGA LGTRLPAAWA PARVALERGV PTLPLPHPGG AVLPVPQVSS ASQEEQDEEL
DHILSPPPMP FRKCSNPDVA CGLGKSLKYK RQLSEDGKQL RRGSLGGALT GRYLLPNPVA
GQAWPASAET SNLVRMRSQA LGQSAPSLTA SLKELSLPRR GSLCRTSNRK SLIGNGQSPA
LPRPHSPLSA HAGNSPQDSP RNFSPSASAH FSFARRTDGR RWSLASLPSS GYGTNTPSST
VSSSCSSQEK LHQLPYQPTP DELHFLSKHF CTTESIATEN RCRNTPMRPR SRSLSPGRSP
ACCDHEIIMM NHVYKERFPK ATAQMEERLK EIITSYSPDH VLPLADGVLS FTHHQIIELA
RDCLDKSHQG LITSRYFFEL QHKLDKLLQE AHDRSESGEL AFIKQLVRKI LIVIARPARL
LECLEFDPEE FYYLLEAAEG HAKEGQGIKT DIPRYIISQL GLNKDPLEEM AQLGNYDSRT
AETPEMDESV SSSNTSLRLR RKPRESDFET IKLISNGAYG AVYFVRHKES RQRFAMKKIN
KQNLILRNQI QQAFVERDIL TFAENPFVVS MYCSFETRRH LCMVMEYVEG GDCATLMKNM
GPLPVDMARM YFAETVLALE YLHNYGIVHR DLKPDNLLVT SMGHIKLTDF GLSKVGLMSM
TTNLYEGHIE KDAREFLDKQ VCGTPEYIAP EVILRQGYGK PVDWWAMGII LYEFLVGCVP
FFGDTPEELF GQVISDEINW PEKDEAPPPD AQELITLLLR QNPLERLGTG GAYEVKQHRF
FRSLDWNSLL RQKAEFIPQL ESEDDTSYFD TRSEKYHHME TEEEDDTNDE DFTVEIRQFS
SCSHRFSKVF SSIDRITQNS GEDKDDSEDK TKSTTLPSTE TLSWSSEYSE MQQLSTSNSS
DTESNRCKLS SGLLPKLAIS TDGEQDEAVP CSGDPREEPE KPVPPSEECT QEEPEVTTPA
STISSSTLSV GSFSEHLDQI NGRSECVDST DNSSKPSSEP TSHVARQRLE STEKKKISGK
VTKSLSASAL SLMIPGDMFA VSPLGSPMSP HSLSSDPSSS RDSSPSRDSS AASASPHQPI
VIHSSGKNYG FTIRAIRVYV GDSDIYTVHH IVWNVEEGSP AYQAGLKAGD LITHINGEPV
HGLVHTEVIE LLLKSGNKVS ITTTPFENTS IKTGPARRNS YKGRMVRRSK KSKKKESLER
RRSLFKKLAK QPSPLLHTSR SFSCLNRSLS SGESLPGSPT HSLSPRSPTP SYRSTPDFPS
GTNSSQSSSP SSSAPNSPAG SGHIRPSTLH GLAPKLSGQR YRSGRRKSAG SIPLSPLART
PSPTPQPTSP QRSPSPLLGH SLGNAKITQA FPSKMHSPPT IVRHIVRPKS AEPPRSPLLK
RVQSEEKLSP SYGSDKKLLC SRKHSLEVTQ EEVQREQCQR EVTLQSLEEN VCDAPSLSRA
RPVEQGCLKR PVSRKVGRQE SVDDLDRDKL KAKVVVKKPE EKHESHQKPH SLGGDSESYA
LFRLEEREKK VYSKGLERSG HFENTSAELP SVGSLLKDTL HKQASVRASE GVTSDGAACS
LTPGEHSQSL GDFKRASASG ILHDSVCPIS DRPAPGKVEY SEKASQAKEL LRSEKLDSKL
ANIDYLRKKM SLDDKDDSHC AILKPKITSS AHECLPGNPI RPMAGQQETP PASENRAFIN
STHTPQMSAV SFVPLKALAG RVENGGEKAG LAAPESPVRK SPSEYKLEGR SVSCLKPIEG
TLDIALLSGP HASKTELLSP EPAQSPSPGI NVGPCVPLAL PGSSGKKGDS TSLREPSSAN
LKVNKSYLLE PRFLPPSRAL QDSLAASGPE PKSKPERKLI HPSARSPATV TESNLQQKEG
GPATHQDRST DTRNLPGPGQ TLHNVDLPRL CTRAPLPPEG TPAKEKPCLK EPSAKVKSEW
SAVRDDGHRD PCAKLCPAET GKASDSSKPL PSGGRTQPDF YKQTQTSEKA WAHAKTNHKD
SQDEVKSLAR EDSASLLYEK EIGRARKGPE PKPEVPATRC PPQPPGIEGE KREKLSAAPS
LQKQAPKEPD RKEQTSQRPG GSGPQQPPPT KELSNSASWQ HGSSPSHTLK KEPGTKAAAA
EPSTSLHDTP RSATATTTAI ATTTTTTSAG HSDCSSHKAR PGPDPSPSKS KHQDRSLSSQ
KLSAGSAKGK EPVTQPLGGS IREGKGGSKG PVDTFSAVLT TQGKASDVLV QGEGRVSIIV
HTEECPLDAK LKNTNGGCPP EMQAKHPPRQ GHLSEAADQK PLIAGEKQSP SPKHPKPSTV
KDYPSLCRQT DRSPSHQATT GDRKAEGKKC TDALYVAAPE GYKPEASPSL HHGETGLRGS
ERPPMGMGKG FSEPKGKGPG PQKSLAETGK PSGMKRSPSA TVQSSLRSAA PPEKSLSYSA
SFPEAQPGVR EVPAANSSPS SAKATGGTSE FPAPSSRDHR KLQSGGDGRS QMIKSDSLPS
FRLSTSALES HFQDPQVPIA SGHRGRALSV TAATGEPKGR ELAQPPPVRK QNACREATRA
PPAPSTDRSL PLSSEKDFVV RQRRGKETLR SSPHKKAS
//
