ID Q837X5_ENTFA Unreviewed; 459 AA.
AC Q837X5;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 140.
DE SubName: Full=Hemolysin {ECO:0000313|EMBL:AAO80521.1};
GN OrderedLocusNames=EF_0700 {ECO:0000313|EMBL:AAO80521.1};
OS Enterococcus faecalis (strain ATCC 700802 / V583).
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=226185 {ECO:0000313|EMBL:AAO80521.1, ECO:0000313|Proteomes:UP000001415};
RN [1] {ECO:0000313|EMBL:AAO80521.1, ECO:0000313|Proteomes:UP000001415}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700802 / V583 {ECO:0000313|Proteomes:UP000001415};
RX PubMed=12663927; DOI=10.1126/science.1080613;
RA Paulsen I., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D.,
RA Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA Dodson R.J., Umayam L., Brinkac L., Beanan M., Daugherty S., DeBoy R.T.,
RA Durkin S., Kolonay J., Madupu R., Nelson W., Vamathevan J., Tran B.,
RA Upton J., Hansen T., Shetty J., Khouri H., Utterback T., Radune D.,
RA Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT faecalis.";
RL Science 299:2071-2074(2003).
RN [2] {ECO:0007829|PDB:2R2Z}
RP X-RAY CRYSTALLOGRAPHY (1.20 ANGSTROMS) OF 346-435 IN COMPLEX WITH ZINC.
RA Zhang R., Tan K., Zhou M., Bargassa M., Joachimiak A.;
RT "The crystal structure of a hemolysin domain from Enterococcus faecalis
RT V583.";
RL Submitted (AUG-2007) to the PDB data bank.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the UPF0053 family.
CC {ECO:0000256|ARBA:ARBA00006337}.
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DR EMBL; AE016830; AAO80521.1; -; Genomic_DNA.
DR RefSeq; NP_814451.1; NC_004668.1.
DR RefSeq; WP_002363168.1; NZ_KE136527.1.
DR PDB; 2R2Z; X-ray; 1.20 A; A=346-435.
DR PDBsum; 2R2Z; -.
DR AlphaFoldDB; Q837X5; -.
DR SMR; Q837X5; -.
DR STRING; 226185.EF_0700; -.
DR DNASU; 1199600; -.
DR EnsemblBacteria; AAO80521; AAO80521; EF_0700.
DR GeneID; 60892987; -.
DR KEGG; efa:EF0700; -.
DR PATRIC; fig|226185.45.peg.2642; -.
DR eggNOG; COG1253; Bacteria.
DR HOGENOM; CLU_015237_4_1_9; -.
DR EvolutionaryTrace; Q837X5; -.
DR Proteomes; UP000001415; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd04590; CBS_pair_CorC_HlyC_assoc; 1.
DR Gene3D; 3.30.465.10; -; 1.
DR Gene3D; 3.10.580.10; CBS-domain; 1.
DR InterPro; IPR000644; CBS_dom.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR002550; CNNM.
DR InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR InterPro; IPR044751; Ion_transp-like_CBS.
DR InterPro; IPR005170; Transptr-assoc_dom.
DR PANTHER; PTHR22777; HEMOLYSIN-RELATED; 1.
DR PANTHER; PTHR22777:SF33; UPF0053 PROTEIN YEGH; 1.
DR Pfam; PF00571; CBS; 2.
DR Pfam; PF01595; CNNM; 1.
DR Pfam; PF03471; CorC_HlyC; 1.
DR SMART; SM01091; CorC_HlyC; 1.
DR SUPFAM; SSF54631; CBS-domain pair; 1.
DR SUPFAM; SSF56176; FAD-binding/transporter-associated domain-like; 1.
DR PROSITE; PS51371; CBS; 2.
DR PROSITE; PS51846; CNNM; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:2R2Z};
KW CBS domain {ECO:0000256|ARBA:ARBA00023122, ECO:0000256|PROSITE-
KW ProRule:PRU00703}; Coiled coil {ECO:0000256|SAM:Coils};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PROSITE-
KW ProRule:PRU01193}; Metal-binding {ECO:0007829|PDB:2R2Z};
KW Reference proteome {ECO:0000313|Proteomes:UP000001415};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|PROSITE-
KW ProRule:PRU01193};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, ECO:0000256|PROSITE-
KW ProRule:PRU01193}; Zinc {ECO:0007829|PDB:2R2Z}.
FT TRANSMEM 12..36
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 66..87
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 7..207
FT /note="CNNM transmembrane"
FT /evidence="ECO:0000259|PROSITE:PS51846"
FT DOMAIN 221..284
FT /note="CBS"
FT /evidence="ECO:0000259|PROSITE:PS51371"
FT DOMAIN 287..344
FT /note="CBS"
FT /evidence="ECO:0000259|PROSITE:PS51371"
FT REGION 440..459
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 300..354
FT /evidence="ECO:0000256|SAM:Coils"
FT BINDING 359
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:2R2Z"
FT BINDING 400
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:2R2Z"
FT BINDING 401
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="3"
FT /evidence="ECO:0007829|PDB:2R2Z"
FT BINDING 403
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="4"
FT /evidence="ECO:0007829|PDB:2R2Z"
SQ SEQUENCE 459 AA; 51271 MW; 1FD7749D918DCEAF CRC64;
MNNADPESQS LIAQLLLLVV LTFINAFLAA AEIAVVSVNK NRVEQKAEDG DAKAQKLLKV
LQDPNNFLST IQVGITLVNI LSGASLAETL SSRLAPVLGG GAAAKSLASI IILALLTYVS
IVFGELYPKR IAMNKSEEVA QLTSGAVRFL GVIARPFVWL LSASTDLLSK ITPMTFDDAD
SKMTRDEMRY MLETEGVLEN EELEMLQGVF SLDTKVAREV MVPRTDAFMV DIQDDVQENI
NLILGENYSR IPVYSEDKDK IVGILHTKTL LKAARNLGFE NIELGAIIQE PLFVPETIFI
DDLLYELKRT QNQMAILLDE YGGVVGLATL EDLLEEIVGE IDDETDEVEN LYTQVADNEY
LVQGRMLIDE FNEVFETDLH MSDVDTMAGY LITALGTIPD EGEKPSFEVG NIKLTAEEME
GTRLLVLRVH FYDEETVDEE PEENRRFFRK EMEDDEPRR
//
