ID FTHS_METEA Reviewed; 557 AA.
AC Q83WS0; C5AS46;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-APR-2013, entry version 50.
DE RecName: Full=Formate--tetrahydrofolate ligase;
DE EC=6.3.4.3;
DE AltName: Full=Formyltetrahydrofolate synthetase;
DE Short=FHS;
DE Short=FTHFS;
GN Name=fhs; Synonyms=ftfL; OrderedLocusNames=MexAM1_META1p0329;
OS Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Methylobacteriaceae; Methylobacterium.
OX NCBI_TaxID=272630;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13, CATALYTIC
RP ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RX PubMed=14645277; DOI=10.1128/JB.185.24.7169-7175.2003;
RA Marx C.J., Laukel M., Vorholt J.A., Lidstrom M.E.;
RT "Purification of the formate-tetrahydrofolate ligase from
RT Methylobacterium extorquens AM1 and demonstration of its requirement
RT for methylotrophic growth.";
RL J. Bacteriol. 185:7169-7175(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14718 / DSM 1338 / AM1;
RX PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A.,
RA Zhou Y., Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C.,
RA Gillett W., Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S.,
RA Muller E., Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G.,
RA Roche D., Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z.,
RA Marx C.J., Vorholt J.A., Olson M.V., Kaul R., Weissenbach J.,
RA Medigue C., Lidstrom M.E.;
RT "Methylobacterium genome sequences: a reference blueprint to
RT investigate microbial metabolism of C1 compounds from natural and
RT industrial sources.";
RL PLoS ONE 4:E5584-E5584(2009).
CC -!- CATALYTIC ACTIVITY: ATP + formate + tetrahydrofolate = ADP +
CC phosphate + 10-formyltetrahydrofolate.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=22 mM for formate;
CC KM=0.8 mM for tetrahydrofolate;
CC KM=21 uM for ATP;
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC -!- SUBUNIT: Homotetramer.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase
CC family.
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DR EMBL; AY279316; AAP33693.1; -; Genomic_DNA.
DR EMBL; CP001510; ACS38281.1; -; Genomic_DNA.
DR RefSeq; YP_002961558.1; NC_012808.1.
DR ProteinModelPortal; Q83WS0; -.
DR SMR; Q83WS0; 4-555.
DR EnsemblBacteria; ACS38281; ACS38281; MexAM1_META1p0329.
DR GeneID; 7990350; -.
DR KEGG; mea:Mex_1p0329; -.
DR PATRIC; 22506475; VBIMetExt101010_0354.
DR eggNOG; COG2759; -.
DR HOGENOM; HOG000040280; -.
DR KO; K01938; -.
DR OMA; IIIGYNY; -.
DR ProtClustDB; CLSK2330235; -.
DR BioCyc; MetaCyc:MONOMER-3942; -.
DR BRENDA; 6.3.4.3; 3296.
DR UniPathway; UPA00193; -.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:HAMAP.
DR GO; GO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR HAMAP; MF_01543; FTHFS; 1; -.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR Pfam; PF01268; FTHFS; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Complete proteome; Direct protein sequencing; Ligase;
KW Nucleotide-binding; One-carbon metabolism.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 557 Formate--tetrahydrofolate ligase.
FT /FTId=PRO_0000199359.
FT NP_BIND 65 72 ATP (By similarity).
SQ SEQUENCE 557 AA; 59391 MW; D0DB51A90971072A CRC64;
MPSDIEIARA ATLKPIAQVA EKLGIPDEAL HNYGKHIAKI DHDFIASLEG KPEGKLVLVT
AISPTPAGEG KTTTTVGLGD ALNRIGKRAV MCLREPSLGP CFGMKGGAAG GGKAQVVPME
QINLHFTGDF HAITSAHSLA AALIDNHIYW ANELNIDVRR IHWRRVVDMN DRALRAINQS
LGGVANGFPR EDGFDITVAS EVMAVFCLAK NLADLEERLG RIVIAETRDR KPVTLADVKA
TGAMTVLLKD ALQPNLVQTL EGNPALIHGG PFANIAHGCN SVIATRTGLR LADYTVTEAG
FGADLGAEKF IDIKCRQTGL KPSAVVIVAT IRALKMHGGV NKKDLQAENL DALEKGFANL
ERHVNNVRSF GLPVVVGVNH FFQDTDAEHA RLKELCRDRL QVEAITCKHW AEGGAGAEAL
AQAVVKLAEG EQKPLTFAYE TETKITDKIK AIATKLYGAA DIQIESKAAT KLAGFEKDGY
GGLPVCMAKT QYSFSTDPTL MGAPSGHLVS VRDVRLSAGA GFVVVICGEI MTMPGLPKVP
AADTIRLDAN GQIDGLF
//
